Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	253,379	T→C	G122G (GGT→GGC)	frsA →	esterase FrsA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	253,379	0	T	C	100.0%	114.0 / NA	31	G122G (GGT→GGC)	frsA	esterase FrsA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (12/19);  total (12/19)

GTGGCGGCAACTGGATTTATGAGTGGGCCACCCAGGCGATGGTGTGGCAACAAAAAGCCTGTGCGGAAGACGATCCGCAACTCAGTGGTCGTCACTGGCTGCATGCGGCTACGTTGTACAACATTGCCGCCTATCCTCATCTGAAAGGAGATGACCTGGCCGAGCAAGCGCAG  >  NZ_CP009273/253291‑253463                                                                                         |                                                                                     gTGGCGGCAACTGGATTTATGAGTGGGCCACCCAGGGGATGGTGTGGCAACAAAAAGCCTGTGCGGAAGACGATCCGCAACTCAGTGgccg                                                                                    <  2:195903/91‑1 (MQ=255)     cggcAACTGGATTTATGAGTGGGCCACCCAGGCGATGGTGTGGCAACAAAAAGCCTGTGCGGAAGACGATCCGCAACTCAGTGGCCGTCAc                                                                                <  1:98424/91‑1 (MQ=255)         aaCTGGATTTATGAGTGGGCCACCCAGGCGATGGTGTGGCAACAAAAAGCCTGTGCGGAAGACGATCCGCAACTCAGTGGCCGTCACTGGc                                                                            <  1:290733/91‑1 (MQ=255)              gATTTATGAGTGGGCCAGCGAGGCGCTGGTGTGTCAACAAAAAGTCTGGGCGGAAGTCGATCCGCAACTCAGTGGCCGTCACTGGCTGCAt                                                                       <  2:446830/91‑1 (MQ=255)              gATTTATGAGTGGGCCACCCAGGCGATGGTGTGGCAACAAAAAGCCTGTGCGGAAGACGATCCGCAACTCAGTGGCCGTCACTGGCTGCAt                                                                       <  1:102011/91‑1 (MQ=255)              gATTTATGAGTGGGCCACCCAGGCGATGGTGTGGCAACAAAAAGCCTGTGCGGAAGACGATCCGCAACTCAGTGGCCGTCACTGGCTGCAt                                                                       <  2:396120/91‑1 (MQ=255)              gATTTATGAGTGGGCCACCCAGGCGATGGTGTGGCAACAAAAAGCCTGTGCGGAAGACGATCCGCAACTCAGTGGCCGTCACTGGCTGCAt                                                                       <  1:870693/91‑1 (MQ=255)              gATTTATGAGTGGGCCACCCAGGCGATGGTGTGGCAACAAAAAGCCTGTGCGGAAGACGATCCGCAACTCAGTGGCCGTCACTGGCTGCAt                                                                       <  2:846680/91‑1 (MQ=255)                      aGTGGGCCACCCAGGCGATGGTGTGGCAACAACAAGCCTGTGCGGAAGACGATCCGCAACTCAGTGGCCGTCACTGGCTGCATGCGGCTAc                                                               <  1:736637/91‑1 (MQ=255)                      aGTGGGCCACCCAGGCGATGGTGTGGCAACAAAAAGCCTGTGCGGAAGACGATCCGCAACTCAGTGGCCGTCACTGGCTGCATGCGGCTAc                                                               <  1:182991/91‑1 (MQ=255)                       gTGGGCCACCCAGGCGATGGTGTGGCAACAAAAAGCCTGTGCGGAAGACGATCCGCAACTCAGTGGCCGTCACTGGCTGCATGCGGCTACg                                                              >  1:359182/1‑91 (MQ=255)                       gTGGGCCACCCAGGCGATGGTGTGGCAACAAAAAGCCTGTGCGGAAGACGATCCGCAACTCAGTGGCCGTCACTGGCTGCATGCGGCTACg                                                              >  2:105558/1‑91 (MQ=255)                        tGGGCCACCCAGGCGATGGTGTGGCAACAAAAAGCCTGTGCGGAAGACGATCCGCAACTCAGTGGCCGTCACTGGCTGCATGCGGCTACGt                                                             >  2:651803/1‑91 (MQ=255)                        tGGGCCACCCAGGCGATGGTGTGGCAACAAAAAGCCTGTGCGGAAGACGATCCGCAACTCAGTGGCCGTCACTGGCTGCATGCGGCTACGt                                                             <  1:301223/91‑1 (MQ=255)                        tGGGCCACCCAGGCGATGGTGTGGCAACAAAAAGCCTGTGCGGAAGACGATCCGCAACTCAGTGGCCGTCACTGGCTGCATGCGGCTACGt                                                             <  2:326156/91‑1 (MQ=255)                         gggCCACCCAGGCGATGGTGTGGCAACAAAAAGCCTGTGCGGAAGACGATCCGCAACTCAGTGGCCGTCACTGGCTGCATGCGGCTACGtt                                                            >  2:457708/1‑91 (MQ=255)                                   ggCGATGGTGTGGCAACAAAAAGCCTGTGCGGAAGACGATCCGCAACTCAGTGGCCGTCACTGGCTGCATGCGGCTACGTTGTACAACAtt                                                  >  1:338408/1‑91 (MQ=255)                                         ggTGTGGCAACAAAAAGCCTGTGCGGAAGACGATCCGCAACTCAGTGGCCGTCACTGGCTGCATGCGGCTACGTTGTACAACATTgccgcc                                            <  1:601146/91‑1 (MQ=255)                                                caacaaAAAGCCTGTGCGGAAGACGATCCGCAACTCAGTGGCCGTCACTGGCTGCATGCGGCTACGTTGTACAACATTGCCGCCTATCCTc                                     <  1:600299/91‑1 (MQ=255)                                                   aaaaaaGCCTGTGCGGAAGACGATCCGCAACTCAGTGGCCGTCACTGGCTGCATGCGGCTACGTTGTACAACATTGCCGCCTATCCTCATc                                  <  2:6498/90‑1 (MQ=255)                                                    aaaaaGCCTGTGCGGAAGACGATCCGCAACTCAGTGGCCGTCACTGGCTGCATGCGGCTACGTTGTACAACATTGCCGCCTATCCTCATCt                                 >  1:99909/1‑91 (MQ=255)                                                        aGCCTGTGCGGAAGACGATCCGCAACTCAGTGGCCGTCACTGGCTGCATGCGGCTACGTTGTACAACATTGCCGCCTATCCTCATCTGaaa                             <  2:99909/91‑1 (MQ=255)                                                        aGCCTGTGCGGAAGACGATCCGCAACTCAGTGGCCGTCACTGGCTGCATGCGGCTACGTTGTACAACATTGCCGCCTATCCTCATCTGaaa                             <  1:651803/91‑1 (MQ=255)                                                         gCCTGTGCGGAAGACGATCCGCAACTCAGTGGCCGTCACTGGCTGCATGCGGCTACGTTGTACAACATTGCCGCCTATCCTCATCTGAAAg                            >  2:809683/1‑91 (MQ=255)                                                            tgtgCGGAAGACGATCCGCAACTCAGTGGCCGTCACTGGCTGCAt                                                                       >  2:158850/1‑45 (MQ=255)                                                            tgtgCGGAAGACGATCCGCAACTCAGTGGCCGTCACTGGCTGCAt                                                                       <  1:158850/45‑1 (MQ=255)                                                             gtgCGGAAGACGATCCGCAACTCAGTGGCCGTCACTGGCTGCATGCGGCTACGTTATACAACATTGCCGCCTATCCTCATCTGAAAGgaga                        >  1:292088/1‑91 (MQ=255)                                                                   aaGACGATCCGCAACTCAGTGGCCGTCACTGGCTGCATGCGGCTACGTTGTACAACATTGCCGCCTATCCTCATCTGAAAGGAGATGACCt                  >  1:800783/1‑91 (MQ=255)                                                                                aCTCAGTGGCCGTCACTGGCTGCATGCGGCTACGTTGTACAACATTGCCGCCTATCCTCATCTGAAAGGAGATGACCTGGCCGAGc          >  1:762829/1‑86 (MQ=255)                                                                                aCTCAGTGGCCGTCACTGGCTGCATGCGGCTACGTTGTACAACATTGCCGCCTATCCTCATCTGAAAGGAGATGACCTGGCCGAGc          <  2:762829/86‑1 (MQ=255)                                                                                 cTCAGTGGCCGTCACTGGCTGCATGCGGCTACGTTGTACAACATTGCCGCCTATCCTCATCTGAAAGGAGATGACCTGGCCGAGCAAgcgc    <  2:854701/91‑1 (MQ=255)                                                                                   cAGTGGCCGTCACTGGCTGCATGCGGCTACGTTGTACAACATTGCCGCCTATCCTCATCTGAAAGGAGATGACCTGGCCGAGCAAGCGCAg  >  2:651623/1‑91 (MQ=255)                                                                                         |                                                                                     GTGGCGGCAACTGGATTTATGAGTGGGCCACCCAGGCGATGGTGTGGCAACAAAAAGCCTGTGCGGAAGACGATCCGCAACTCAGTGGTCGTCACTGGCTGCATGCGGCTACGTTGTACAACATTGCCGCCTATCCTCATCTGAAAGGAGATGACCTGGCCGAGCAAGCGCAG  >  NZ_CP009273/253291‑253463

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑