| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 2,666,371 | T→A | D322E (GAT→GAA) | hcaD → | phenylpropionate dioxygenase ferredoxin reductase subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,666,371 | 0 | T | A | 100.0% | 47.2 / NA | 13 | D322E (GAT→GAA) | hcaD | phenylpropionate dioxygenase ferredoxin reductase subunit |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base A (7/6); total (7/6) | |||||||||||
AACCAGGCGCAAATTGCCGCTGCCGCAATGTTAGGGCTACCGCTACCGCTACTGCCGCCGCCGTGGTTCTGGAGCGATCAGTACAGTGATAACTTACAGTTTATTGGCGATATGCGTGGCGATGACTGGCTTTGTCGTGGCAACCCGGAAACTCAGAAGGCGATT > NZ_CP009273/2666294‑2666458 | aaCCAGGCGCAAATTGCCGCTGCCGCAATGTTAGGGCTACCGCTACCGCCACTGCCGCCGCCGTGGTTCTGGAGCGAACAGTACAGTGATa < 1:30804/91‑1 (MQ=255) ctaccgctaccgCTACTGCCGCCGCCGTGGTTCTGGAGCGAACAGTACAGTGATAACTTACAGTTTATTGGCGATATGCGTGGCGATGACt < 1:465431/91‑1 (MQ=255) taccgctaccgcTACTGCCGCCGCCGTGGTTCTGGAGCGAACAGTACAGTGATAACTTACAGTTTATTGGCGATATGCGTGGCGATGACTg > 1:370270/1‑91 (MQ=255) taccgctaccgcTACTGCCGCCGCCGTGGTTCTGGAGCGAACAGTACAGTGATAACTTACAGTTTATTGGCGATATGCGTGGCGATGACTg > 2:36630/1‑91 (MQ=255) cgctaccgctacTGCCGCCGCCGTGGTTCTGGAGCGAACAGTACAGTGATAACTTACAGTTTATTGGCGATATGCGTGGCGATGACTGGCt > 2:438225/1‑91 (MQ=255) accgctacTGCCGCCGCCGTGGTTCTGGAGCGAACAGTACAGTGATAACTTACa > 1:84374/1‑54 (MQ=255) accgctacTGCCGCCGCCGTGGTTCTGGAGCGAACAGTACAGTGATAACTTACa < 2:84374/54‑1 (MQ=255) gctacTGCCGCCGCCGTGGTTCTGGAGCGAACAGTACAGTGATAACTTACAGTTTATTGGCGATATGCGTGGCGATGACTGGCTTTGTCGt > 2:677770/1‑91 (MQ=255) tacTGCCGCCGCCGTGGTTCTGGAGCGAACAGTACAGTGATAACTTACAGTTTATTGGCGATATGCGTGGCGATGACTGGCTTTGTCGTgg > 2:195610/1‑91 (MQ=255) cTGCCGCCGCCGTGGTTCTGGAGCGAACAGTACAGTGATAACTTACAGTTTATTGGCGATATGCGTGGCGATGACTGGCTTTGTCGTGGCa < 1:36630/91‑1 (MQ=255) gccgccgTGGTTCTGGAGCGAACAGTACAGTGATAACTTACAGTTTATTGGCGATATGCGTGGCGATGACTGGCTTTGTCGTGGCAACCCg > 2:621687/1‑91 (MQ=255) ttCTGGAGCGAACAGTACAGTGATAACTTACAGTTTATTGGCGATATGCGTGGCGATGACTGGCTTTGTCGTGGCAACCCGGAAACTCAGa < 2:509180/91‑1 (MQ=255) cGAACAGTACAGTGATAACTTACAGTTTATTGGCGATATGCGTGGCGATGACTGGCTTTGTCGTGGCAACCCGGAAACTCAGAAGGCGAtt < 1:195610/91‑1 (MQ=255) | AACCAGGCGCAAATTGCCGCTGCCGCAATGTTAGGGCTACCGCTACCGCTACTGCCGCCGCCGTGGTTCTGGAGCGATCAGTACAGTGATAACTTACAGTTTATTGGCGATATGCGTGGCGATGACTGGCTTTGTCGTGGCAACCCGGAAACTCAGAAGGCGATT > NZ_CP009273/2666294‑2666458 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |