Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,684,806	T→C	intergenic (‑349/+209)	qseE ← / ← purL	two component system sensor histidine kinase QseE/GlrK/phosphoribosylformylglycinamidine synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,684,806	0	T	C	100.0%	92.8 / NA	25	intergenic (‑349/+209)	qseE/purL	two component system sensor histidine kinase QseE/GlrK/phosphoribosylformylglycinamidine synthase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (15/10);  total (15/10)

GTGCCAATAAAGAAAATAGTTTGGTAACGTACTGATTATACGTTGCTTTGAGGGGTTTATGTCTCCTCCGCTGTTTGAATGTAAATCAGCCACTGTGTCGCTAAAAAGAGACAACTTAAGATAAACTTATTAGATAATATTAAAATCAATGAGTTAAGTGTCGCC  >  NZ_CP009273/2684721‑2684885                                                                                      |                                                                                gtgCCAATAAAGAAAATAGTTTGGTAACGTACTGATTATACGTTGCTTTGAGGGGTTTATGTCTCCTCCGCTGTTTGAATGTAAACCAGcc                                                                            <  1:564220/91‑1 (MQ=255)           aGAAAATAGTTTGGTAACGTACTGATTATACGTTGCTTTGAGGGGTTTATGTCTCCTCCGCTGTTTGAATGTAAACCAGCCACTGTGTCGc                                                                  >  1:826373/1‑91 (MQ=255)                  aGTTTGGTAACGTACTGATTATACGTTGCTTTGAGGGGTTTATGTCTCCTCCGCTGTTTGAATGTAAACCAGCCACTGTGTCGCTAAAAag                                                           >  2:105735/1‑91 (MQ=255)                      tGGTAACGTACTGATTATACGTTGCTTTGAGGGGTTTATGTCTCCTCCGCTGTTTGAATGTAAACCAGCCACTGTGTCGCTAAAAAGAGAc                                                       <  2:684432/91‑1 (MQ=255)                      tGGTAACGTACTGATTATACGTTGCTTTGAGGGGTTTATGTCTCCTCCGCTGTTTGAATGTAAACCAGCCACTGTGTCGCTAAAAAGAGAc                                                       <  1:563416/91‑1 (MQ=255)                      tGGTAACGTACTGATTATACGTTGCTTTGAGGGGTTTATGTCTCCTCCGCTGTTTGAATGTAAACCAGCCACTGTGTCGCTAAAAAGAGAc                                                       <  2:4425/91‑1 (MQ=255)                             gTACTGATTATACGTTGCTTTGAGGGGTTTATGTCTCCTCCGCTGTTTGAATGTAAACCAGCCACTGTGTCGCTAAAAAGAGACAACTTaa                                                >  1:432906/1‑91 (MQ=255)                             gTACTGATTATACGTTGCTTTGAGGGGTTTATGTCTCCTCCGCTGTTTGAATGTAAACCAGCCACTGTGTCGCTAAAAAGAGACAACTTaa                                                >  2:191026/1‑91 (MQ=255)                                       taCGTTGCTTTGAGGGGTTTATGTCTCCTCCGCTGTTTGAATGTAAACCa                                                                               <  1:811176/50‑1 (MQ=255)                                            tGCTTTGAGGGGTTTATGTCTCCTCCGCTTTTTGAATGTAAACCAGCCACTGTGTCGCTAAAAAGAGACAACTTAAGATa                                            <  2:736059/80‑1 (MQ=255)                                            tGCTTTGAGGGGTTTATGTCTCCTCCGCTGTTTGAATGTAAACCAGCCACTGTGTCGCTAAAAAGAGACAACTTAAGATa                                            <  2:830558/80‑1 (MQ=255)                                            tGCTTTGAGGGGTTTATGTCTCCTCCGCTGTTTGAATGTAAACCAGCCACTGTGTCGCTAAAAAGAGACAACTTAAGATa                                            >  1:830558/1‑80 (MQ=255)                                            tGCTTTGAGGGGTTTATGTCTCCTCCGCTGTTTGAATGTAAACCAGCCACTGTGTCGCTAAAAAGAGACAACTTAAGATa                                            >  1:736059/1‑80 (MQ=255)                                            tGCTTTGAGGGGTTTATGTCTCCTCCGCTGTTTGAATGTAAACCAGCCACTGTGTCGCTAAAAAGAGACAACTTAAGATAAACTTATTAGa                                 >  2:183231/1‑91 (MQ=255)                                            tGCTTTGAGGGGTTTATGTCTCCTCCGCTGTTTGAATGTAAACCAGCCACTGTGTCGCTAAAAAGAGACAACTTAAGATAAACTTATTAGa                                 >  2:421115/1‑91 (MQ=255)                                            tGCTTTGAGGGGTTTATGTCTCCTCCGCTGTTTGAATGTAAACCAGCCACTGTGTCGCTAAAAAGAGACAACTTAAGATAAACTTATTAGa                                 >  2:429221/1‑91 (MQ=255)                                            tGCTTTGAGGGGTTTATGTCTCCTCCGCTGTTTGAATGTAAACCAGCCACTGTGTCGCTAAAAAGAGACAACTTAAGATAAACTTATTAGa                                 >  2:530401/1‑91 (MQ=255)                                            tGCTTTGAGGGGTTTATGTCTCCTCCGCTGTTTGAATGTAAACCAGCCACTGTGTCGCTAAAAAGAGACAACTTAAGATAAACTTATTAGa                                 >  2:582684/1‑91 (MQ=255)                                            tGCTTTGAGGGGTTTATGTCTCCTCCGCTGTTTGAATGTAAACCAGCCACTGTGTCGCTAAAAAGAGACAACTTAAGATAAACTTATTAGa                                 >  2:722737/1‑91 (MQ=255)                                             gCTTTGAGGGGTTTATGTCTCCTCCGCTGTTTGAATGTAAACCAGCCACTGTGTCGCTAAAAAGAGACAACTTAAGATAAACTTATTAGat                                <  1:581746/91‑1 (MQ=255)                                             gCTTTGAGGGGTTTATGTCTCCTCCGCTGTTTGAATGTAAACCAGCCACTGTGTCGCTAAAAAGAGACAACTTAAGATAAACTTATTAGat                                >  1:22599/1‑91 (MQ=255)                                                ttGAGGGGTTTATGTCTCCTCCGCTGTTTGAATGTAAACCAGCCACTGTGTCGCTAAAAAGAGACAACTTAAGATAAACTTATTAGataat                             >  1:598355/1‑91 (MQ=255)                                                  gAGGGGTTTATGTCTCCTCCGCTGTTTGAATGTAAACCAGCCACTGTGTCGCTAAAAAGAGACAACTTAAGATAAACTTATTAGATAatat                           >  2:503799/1‑91 (MQ=255)                                                     gggTTTATGTCTCCTCCGCTGTTTGAATGTAAACCAGCCACTGTGTCGCTAAAAAGAGACAACTTAAGATAAACTTATTAGATAATATTaa                        <  1:429221/91‑1 (MQ=255)                                                                           ttGAATGTAAACCAGCCACTGTGTCGCTAAAAAGAGACAACTTAAGATAAACTTATTAGATAATATTAAAATCAATGAGTTAAGTGTCGcc  <  1:455644/91‑1 (MQ=255)                                                                                      |                                                                                GTGCCAATAAAGAAAATAGTTTGGTAACGTACTGATTATACGTTGCTTTGAGGGGTTTATGTCTCCTCCGCTGTTTGAATGTAAATCAGCCACTGTGTCGCTAAAAAGAGACAACTTAAGATAAACTTATTAGATAATATTAAAATCAATGAGTTAAGTGTCGCC  >  NZ_CP009273/2684721‑2684885

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑