Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,772,143	A→G	S1315P (TCC→CCC)	ypjA ←	adhesin‑like autotransporter YpjA/EhaD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,772,143	0	A	G	100.0%	109.6 / NA	30	S1315P (TCC→CCC)	ypjA	adhesin‑like autotransporter YpjA/EhaD
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (13/17);  total (13/17)

CTTTCATGTTCCCAACTGGCATAGCCGCCCAGAGAATAACTGCCCACACTGCCATGTCCTCCGCGATCAAAACCGATATGTGAATGGGAATAGCCCATAAAAGCGCCTAGCGTGGTAATTCCTTCAGGAATATCATTACGGCTGTCGATCCCCACTGTCATTCCGGTCAGCGTCT  >  NZ_CP009273/2772055‑2772229                                                                                         |                                                                                       cTTTCATGTTCCCAACTGGCATAGCCGCCCAGAGAATAACTGCCCACACTGCCATGTCCTCCGCGATCAAAACCGATATGTGAATGGGGAt                                                                                      <  2:567367/91‑1 (MQ=255) cTTTCATGTTCCCAACTGGCATAGCCGCCCAGAGAATAACTGCCCACACTGCCATGTCCTCCGCGATCAAAACCGATATGTGAATGGGGAt                                                                                      <  1:559571/91‑1 (MQ=255) cTTTCATGTTCCCAACTGGCATAGCCGCCCAGAGAATAACTGCCCACACTGCCATGTCCTCCGCGATCAAAACCGATATGTGAATGGGGAt                                                                                      <  1:628733/91‑1 (MQ=255) cTTTCATGTTCCCAACTGGCATAGCCGCCCAGAGAATAACTGCCCACACTGCCATGTCCTCCGCGATCAAAACCGATATGTGAATGGGGAt                                                                                      <  1:826684/91‑1 (MQ=255)        gTTCCCAACTGGCATAGCCGCCCAGAGAATAACTGCCCACACTGCCATGTCCTCCGCGATCAAAACCGATATGTGAATGGGGATAGCCCAt                                                                               >  1:120037/1‑91 (MQ=255)            ccAACTGGCATAGCCGCCCAGAGAATAACTGCCCACACTGCCATGTCCTCCGCGATCAAAACCGATATGTGAATGGGGATAGCCCATaaaa                                                                           <  2:489511/91‑1 (MQ=255)            ccAACTGGCATAGCCGCCCAGAGAATAACTGCCCACACTGCCATGTCCTCCGCGATCAAAACCGATATGTGAATGGGGATAGCCCATaaaa                                                                           <  2:404737/91‑1 (MQ=255)                  ggCATAGCCGCCCAGAGAATAACTGCCCACACTGCCATGTCCTCCGCGATCAAAACCGATATGTGAATGGGGATAGCCCATAAAAGCGCCt                                                                     >  2:806524/1‑91 (MQ=255)                            cccAGAGAATAACTGCCCACACTGCCATGTCCTCCGCGATCAAAACCGATATGTGAATGGGGATAGCCCATAAAAGCGCCTAGCGTGGTaa                                                           >  1:859232/1‑91 (MQ=255)                                          gCCCACACTGCCATGTCCTCCGCGATCAAAACCGATATGTGAATGGGGATAGCCCATAAAAGCGCCTAGCGTGGTAATTCCt                                                      >  1:129317/1‑82 (MQ=255)                                          gCCCACACTGCCATGTCCTCCGCGATCAAAACCGATATGTGAATGGGGATAGCCCATAAAAGCGCCTAGCGTGGTAATTCCt                                                      <  2:129317/82‑1 (MQ=255)                                          gCCCACACTGCCATGTCCTCCGCGATCAAAACCGATATGTGAATGGGGATAGCCCATAAAAGCGCCTAGCGTGGTAATTCCTTCAGGAata                                             <  2:472337/91‑1 (MQ=255)                                          gCCCACACTGCCATGTCCTCCGCGATCAAAACCGATATGTGAATGGGGATAGCCCATAAAAGCGCCTAGCGTGGTAATTCCTTCAGGAata                                             <  2:222586/91‑1 (MQ=255)                                          gCCCACACTGCCATGTCCTCCGCGATCAAAACCGATATGTGAATGGGGATAGCCCATAAAAGCGCCTAGCGTGGTAATTCCTTCAGGAata                                             <  1:761841/91‑1 (MQ=255)                                            ccACACTGCCATGTCCTCCGCGATCAAAACCGATATGTGAATGGGGATAGCCCATAAAAGCGCCTAGCGTGGTAATTCCTTCAGGAATATc                                           >  1:706322/1‑91 (MQ=255)                                                    ccATGTCCTCCGCGATCAAAACCGATATGTGAATGGGGATAGCCCATAAAAGCGCCTAGCGTGGTAATTCCTTCAGGAATATCTTTACGGc                                   <  1:811227/91‑1 (MQ=255)                                                         tcctccGCGATCAAAACCGATATGTGAATGGGGATAGCCCATAAAAGCGCCTAGCGTGGTAATTCCTTCAGGAATATCATTACGGCTGTCg                              >  2:56536/1‑91 (MQ=255)                                                          cctccGCGATCAAAACCGATATGTGAATGGGGATAGCCCATAAAAGCGCCTAGCGTGGTAATTCCTTCAGGAATATCATTACGGCTGTCGa                             <  2:57316/91‑1 (MQ=255)                                                             ccGCGATCAAAACCGATATGTGAATGGGGATAGCCCATAAAAGCGCCTAGCGTGGTAATTCCTTCAGGAATATCATTACGGCTGTCGATcc                          >  1:841593/1‑91 (MQ=255)                                                             ccGCGATCAAAACCGATATGTGAATGGGGATAGCCCATAAAAGCGCCTAGCGTGGTAATTCCTTCAGGAATATCATTACGGCTGTCGATcc                          >  1:257397/1‑91 (MQ=255)                                                             ccGCGATCAAAACCGATATGTGAATGGGGATAGCCCATAAAAGCGCCTAGCGTGGTAATTCCTTCAGGAATATCATTACGGCTGTCGATcc                          >  2:583996/1‑91 (MQ=255)                                                               gcgATCAAAACCGATATGTGAATGGGGATAGCCCATAAAAGCGCCTAGCGTGGTAATTCCTTCAGGAATATCATTACGGCTGTCGATcccc                        <  2:203049/91‑1 (MQ=255)                                                               gcgATCAAAACCGATATGTGAATGGGGATAGCCCATAAAAGCGCCTAGCGTGGTAATTCCTTCAGGAATATCATTACGGCTGTCGATcccc                        <  2:538592/91‑1 (MQ=255)                                                               gcgATCAAAACCGATATGTGAATGGGGATAGCCCATAAAAGCGCCTAGCGTGGTAATTCCTTCAGGAATATCATTACGGCTGTCGATcccc                        <  1:148925/91‑1 (MQ=255)                                                                  aTCAAAACCGATATGTGAATGGGGATAGCCCATAAAAGCGCCTAGCGTGGTAATTCCTTCAGGAATATCATTACGGCTGTCGATCCCCACt                     >  2:413309/1‑91 (MQ=255)                                                                            atatGTGAATGGGGATAGCCCATAAAAGCGCCTAGCGTGGTAATTCCTTCAGGACTATCATTCCGGCTGTCGATCCCCCCTGTCATTCCgg           >  2:559924/1‑91 (MQ=255)                                                                            atatGTGAATGGGGATAGCCCATAAAAGCGCCTAGCGTGGTAATTCCTTCAGGAATATCATTACGGCTGTCGATCCCCACTGTCATTCCgg           >  2:253464/1‑91 (MQ=255)                                                                               tgtgAATGGGGATAGCCCATAAAAGCGCCTAGCGTGGTAATTCCTTCAGGAATATCATTACGGCTGTCGATCCCCACTGTCATTCCGGTCa        <  2:460943/91‑1 (MQ=255)                                                                                gtgAATGGGGATAGCCCATAAAAGCGCCTAGCGTGGTAATTCCTTCAGGAATATCATTACGGCTGTCGATCCCCACTGTCATTCCGGTCAg       >  2:68329/1‑91 (MQ=255)                                                                                     tGGGGATAGCCCATAAAAGCGCCTAGCGTGGTAATTCCTTCAGGAATATCATTACGGCTGTCGATCCCCACTGTCATTCCGGTCAGCGTCt  <  2:728166/91‑1 (MQ=255)                                                                                         |                                                                                       CTTTCATGTTCCCAACTGGCATAGCCGCCCAGAGAATAACTGCCCACACTGCCATGTCCTCCGCGATCAAAACCGATATGTGAATGGGAATAGCCCATAAAAGCGCCTAGCGTGGTAATTCCTTCAGGAATATCATTACGGCTGTCGATCCCCACTGTCATTCCGGTCAGCGTCT  >  NZ_CP009273/2772055‑2772229

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑