Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,891,751	T→G	T343P (ACC→CCC)	ygcU ←	FAD‑binding oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,891,751	0	T	G	100.0%	108.2 / NA	30	T343P (ACC→CCC)	ygcU	FAD‑binding oxidoreductase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (17/13);  total (17/13)

AATACGGTTAATAACGCTTTCGTAGATTTCGTGGATGCAGCTCCAGCAGCCGGATACTTCGGTGGTAAAGCCCATGTTGCCGGTTTTGAGGATCTGCACACGTTCGGCAGCCACTTTATCCGGTCCCCAGTTCAGGTTGTTAAACCAGGTTTCGATCAGCTTGCTGTCC  >  NZ_CP009273/2891668‑2891836                                                                                    |                                                                                      aaTACGGTTAATAACGCTTTCGTAGATTTCGTGGATGCAGCTCCAGCAGCCGGANNCTNNGGTGGTAAAGCCCATGTTGCCGGGTTTGAgg                                                                                >  1:211099/1‑91 (MQ=255)    aCGGTTAATAACGCTTTCGTAGATTTCGTGGATGCAGCTCCAGCAGCCGGATACTTCGGTGGTAAAGCCCATGTTGCCGGGTTTGAGGATc                                                                             >  1:683454/1‑91 (MQ=255)       gTTAATAACGCTTTCGTAGATTTCGTGGATGCAGCTCCAGCAGCCGGATACTTCGGTGGTAAAGCCCATGTTGCCGGGTTTGAGGATCTGc                                                                          >  2:402753/1‑91 (MQ=255)         taataaCGCTTTCGTAGATTTCGTGGATGCAGCTCCAGCAGCCGGATACTTCGGTGGTAAAGCCCATGTTGCCGGGTTTGAGGATCTGcac                                                                        <  1:500263/91‑1 (MQ=255)               cGCTTTCGTAGATTTCGTGGATGCAGCTCCAGCAGCCGGATACTTCGGTGGTAAAGCCCATGTTGCCGGGTTTGAGGATCTGCACACGTTc                                                                  >  1:669687/1‑91 (MQ=255)                 cTTTCGTAGATTTCGTGGATGCAGCTCCAGCAGCCGGATACTTCGGTGGTAAAGCCCATGTTGCCGGGTTTGAGGATCTGCACACGTTCgg                                                                >  2:303702/1‑91 (MQ=255)                           tttCGTGGATGCAGCTCCAGCAGCCGGATACTTCGGTGGTAAAGCCCATGTTGCCGGGTTTGAGGATCTGcac                                                                        >  2:92542/1‑73 (MQ=255)                           tttCGTGGATGCAGCTCCAGCAGCCGGATACTTCGGTGGTAAAGCCCATGTTGCCGGGTTTGAGGATCTGcac                                                                        <  1:92542/73‑1 (MQ=255)                             tCGTGGATGCAGCTCCAGCAGCCGGATACTTCGGTGGTAAAGCCCATGTTGCCGGGTTTGAGGATCTGCACACGTTCGGCAGCCACTTTAt                                                    >  1:43420/1‑91 (MQ=255)                             tCGTGGATGCAGCTCCAGCAGCCGGATACTTCGGTGGTAAAGCCCATGTTGCCGGGTTTGAGGATCTGCACACGTTCGGCAGCCACTTTAt                                                    >  2:479803/1‑91 (MQ=255)                                 ggATGCAGCTCCAGCAGCCGGATACTTCGGTGGTAAAGCCCATGTTGCCGGGTTTGAGGATCTGCACACGTTCGGCAGCCACTTTATCCgg                                                <  1:249877/91‑1 (MQ=255)                                    tGCAGCTCCAGCAGCCGGATACTTCGGTGGTAAAGCCCATGTTGCCGGGTTTGAGGATCTGCACACGTTCGGCAGCCACTTTATCCGGTcc                                             <  1:468352/91‑1 (MQ=255)                                    tGCAGCTCCAGCAGCCGGATACTTCGGTGGTAAAGCCCATGTTGCCGGGTTTGAGGATCTGCACACGTTCGGCAGCCACTTTATCCGGTcc                                             <  2:43420/91‑1 (MQ=255)                                     gCAGCTCCAGCAGCCGGATACTTCGGTGGTAAAGCCCATGTTGCCGGGTTTGAGGATCTGCACACGTTCGGCAGCCACTTTATCCGGTccc                                            <  1:479803/91‑1 (MQ=255)                                      cAGCTCCAGCAGCCGGATACTTCGGTGGTAAAGCCCATGTTGCCGGGTTTGAGGATCTGCACACGTTCGGCAGCCACTTTATCCGGTcccc                                           <  1:538038/91‑1 (MQ=255)                                             agcagcCGGATACTTCGGTGGTAAAGCCCATGTTGCCGGGTTTGAGGATCTGCACACGTTCGGCAGCCACTTTATCCGGTCCCCAGTTCAg                                    >  2:623480/1‑91 (MQ=255)                                              gcagcCGGATACTTCGGTGGTAAAGCCCATGTTGCCGGGTTTGAGGATCTGCACCCGTTCGGCAGCCACTTTATCCGGTCCCCAGTTCAgg                                   <  2:727419/91‑1 (MQ=255)                                                agcCGGATACTTCGGTGGTAAAGCCCATGTTGCCGGGTTTGAGGATCTGCACACGTTCGGCAGCCACTTTATCCGGTCCCCAGTTCAGgtt                                 >  2:633167/1‑91 (MQ=255)                                                             ggtggtAAAGCCCATGTTGCCGGGTTTGAGGATCTGCACACGTTCGGCAGCCACTTTATCCGGTCCCCAGTTCAGGTTGTTAAACCAGGtt                    <  1:576221/91‑1 (MQ=255)                                                                   aaaGCCCATGTTGCCGGGTTTGAGGATCTGCACACGTTCGGCAGCCACTTTATCCGGTCCCCAGTTCAGGTTGTTAAACCAGGTTTCGATc              >  2:773804/1‑91 (MQ=255)                                                                       cccATGTTGCCGGGTTTGAGGATCTGCACACGTTCGGCAGCCACTTTATCCGGTCCCCAGTTCAGGTTGTTAAACCAGGGTGCGATCAGCt          <  2:669687/91‑1 (MQ=255)                                                                        ccATGTTGCCGGGTTTGAGGATCTGCACACGTTCGGCAGCCACTTTATCCGGTCCCCAGTTCAGGTTGTTAAACCAGGTTTCGATCAGCtt         >  1:598478/1‑91 (MQ=255)                                                                          aTGTTGCCGGGTTTGAGGATCTGCACACGTTCGGCAGCCACTTTATCCGGTCCCCAGTTCAGGTTGTTAAACCAGGTTTCGATCAGCTTGc       >  1:432071/1‑91 (MQ=255)                                                                          aTGTTGCCGGGTTTGAGGATCTGCACACGTTCGGCAGCCACTTTATCCGGTCCCCAGTTCAGGTTGTTAAACCAGGTTTCGATCAGCTTGc       >  1:126050/1‑91 (MQ=255)                                                                               gCCGGGTTTGAGGATCTGCACACGTTCGGCAGCCACTTTATCCGGTCCCCAGTTCAGGTTGTTAAACCAGGTTTCGATCAGCTTGCTGTcc  >  2:41250/1‑91 (MQ=255)                                                                               gCCGGGTTTGAGGATCTGCACACGTTCGGCAGCCACTTTATCCGGTCCCCAGTTCAGGTTGTTAAACCAGGTTTCGATCAGCTTGCTGTcc  <  2:583499/91‑1 (MQ=255)                                                                                ccGGGTTTGAGGATCTGCACACGTTCGGCAGCCACTTTATCCGGTCCCCAGTTCAGgt                                  >  1:774363/1‑58 (MQ=255)                                                                                ccGGGTTTGAGGATCTGCACACGTTCGGCAGCCACTTTATCCGGTCCCCAGTTCAGgt                                  <  2:774363/58‑1 (MQ=255)                                                                                ccGGGTTTGAGGATCTGCACACGTTCGGCAGCCACTTTATCCGGTCCCCAGTTCAGGTTGTTAAACCAGGTTTCGATCAGCTTGc       <  1:82767/85‑1 (MQ=255)                                                                                ccGGGTTTGAGGATCTGCACACGTTCGGCAGCCACTTTATCCGGTCCCCAGTTCAGGTTGTTAAACCAGGTTTCGATCAGCTTGc       >  2:82767/1‑85 (MQ=255)                                                                                    |                                                                                      AATACGGTTAATAACGCTTTCGTAGATTTCGTGGATGCAGCTCCAGCAGCCGGATACTTCGGTGGTAAAGCCCATGTTGCCGGTTTTGAGGATCTGCACACGTTCGGCAGCCACTTTATCCGGTCCCCAGTTCAGGTTGTTAAACCAGGTTTCGATCAGCTTGCTGTCC  >  NZ_CP009273/2891668‑2891836

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑