Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,931,836	A→G	D337G (GAT→GGT)	fucK →	L‑fuculokinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,931,836	0	A	G	100.0%	98.7 / NA	28	D337G (GAT→GGT)	fucK	L‑fuculokinase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (12/16);  total (12/16)

GGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGATGGCGTAAAAATGCAGTGTGATTTATTGTCGTGTCAGAACGCTGGCTGGCAAGGAGTGACGCTTAATACCACGCGGGGG  >  NZ_CP009273/2931750‑2931915                                                                                       |                                                                                ggAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGGTGGc                                                                             <  1:349040/91‑1 (MQ=255) ggAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGGTGGc                                                                             <  1:81636/91‑1 (MQ=255)      gggTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGGTGGGGTaaa                                                                        >  2:338817/1‑91 (MQ=255)      gggTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGGTGGCGTaaa                                                                        >  1:484597/1‑91 (MQ=255)       ggTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGGTGGCGTaaaa                                                                       >  2:229616/1‑91 (MQ=255)           agaAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGGTGGCGTAAAAATGc                                                                   <  2:611047/91‑1 (MQ=255)           agaAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGGTGGCGTAAAAATGc                                                                   <  1:336580/91‑1 (MQ=255)             aaaaCTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGGTGGCGTAAAAATGCAg                                                                 <  2:834414/91‑1 (MQ=255)             aaaaCTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGGTGGCGTAAAAATGCAg                                                                 <  2:408793/91‑1 (MQ=255)             aaaaCTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGGTGGCGTAAAAATGCAg                                                                 <  1:363921/91‑1 (MQ=255)               aaCTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGGTGGCGTAAAAATGCAgtg                                                               <  1:49768/91‑1 (MQ=255)                  tGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGGTGGCGTAAAAATGCAGTGTGa                                                            >  1:130594/1‑91 (MQ=255)                  tGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGGTGGCGTAAAAATGCAGTGTGa                                                            <  2:405349/91‑1 (MQ=255)                        ggACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGGTGGCGTAAAAATGCAGTGTGATTTAtt                                                      >  2:2877/1‑91 (MQ=255)                          aCGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGGTGGCGTAAAAATGCAGTGTGATTTATTGt                                                    >  1:146656/1‑91 (MQ=255)                           cGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGGTGGCGTAAAAATGCAGTGTGATTTATTGTc                                                   <  2:130594/91‑1 (MQ=255)                                       ccTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGGTGGCGTAAAAATGCAGTGTGATTTATTGTCGTGTCAGAACgc                                       >  2:147780/1‑91 (MQ=255)                                       ccTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGGTGGCGTAAAAATGCAGTGTGATTTATTGTCGTGTCAGAACgc                                       <  2:195294/91‑1 (MQ=255)                                       ccTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGGTGGCGTAAAAATGCAGTGTGATTTATTGTCGTGTCAGAACgc                                       <  1:660625/91‑1 (MQ=255)                                       ccTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGGTGGCGTAAAAATGCAGTGTGATTTATTGTCGTGTCAGAACgc                                       >  1:339246/1‑91 (MQ=255)                                        cTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGGTGGGGTAAAAATGCAGTGTGGTTTATTGTCGTGTCAGAACgct                                      >  2:372590/1‑91 (MQ=255)                                                tGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGGTGGCGTAAAAATGCAGTGTGATTTATTGTCGTGTCAGAACGCTGGCTGGCa                              <  2:807490/91‑1 (MQ=255)                                                    gattgaAGAAGCTCGTCTGATCGCGCCTGGCGCGGGTGGCGTAAAAATGCAGTGTGATTTATTGTCGTGTCAGAACGCTGGCTGGCAAGGa                          >  1:818870/1‑91 (MQ=255)                                                    gattgaAGAAGCTCGTCTGATCGCGCCTGGCGCGGGTGGCGTAAAAATGCAGTGTGATTTATTGTCGTGTCAGAACGCTGGCTGGCAAGGa                          >  1:149288/1‑91 (MQ=255)                                                        gaagaaGCTCGTCTGATCGCGCCTGGCGCGGGTGGCGTAAAAATGCAGTGTGATTTATTGTCGTGTCAGAACGCTGGCTGGCAAGGAGTGa                      <  1:620646/91‑1 (MQ=255)                                                        gaagaaGCTCGTCTGATCGCGCCTGGCGCGGGTGGCGTAAAAATGCAGTGTGATTTATTGTCGTGTCAGAACGCTGGCTGGCAAGGAGTGa                      <  1:176773/91‑1 (MQ=255)                                                              gCTCGTCTGATCGCGCCTGGCGCGGGTGGCGTAAAAATGCAGTGTGATTTATTGTCGTGTCAGAACGCTGGCTGGCAAGGAGTGACGCTTa                >  1:702313/1‑91 (MQ=255)                                                                      gATCGCGCCTGGCGCGGGTGGCGTAAAAATGCAGTGTGATTTATTGTCGTGTCAGAACGCTGGCTGGCAAGGAGTGACGCTTAATACCAcg        >  2:351729/1‑91 (MQ=255)                                                                           cgcCTGGCGCGGGTGGCGTAAAAATGCAGTGTGATTTATTGTCGTGTCAGAACGCTGGCTGGCAAGGAGTGACGCTTAATACCACGCgggg   <  2:146231/91‑1 (MQ=255)                                                                            gcCTGGCGCGGGGGGCGTAAAAATGCAGTGTGATTTATTGTCGTGTCAGAACGCTGGCTGGCAAGTAGTGACGCTTAATACCACGCcgggg  >  1:420137/1‑91 (MQ=255)                                                                                       |                                                                                GGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGATGGCGTAAAAATGCAGTGTGATTTATTGTCGTGTCAGAACGCTGGCTGGCAAGGAGTGACGCTTAATACCACGCGGGGG  >  NZ_CP009273/2931750‑2931915

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑