Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,959,152	A→G	F82S (TTC→TCC)	lgt ←	prolipoprotein diacylglyceryl transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,959,152	0	A	G	100.0%	93.1 / NA	25	F82S (TTC→TCC)	lgt	prolipoprotein diacylglyceryl transferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (12/13);  total (12/13)

ACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGTCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAGAACAGAACATAACCAATACGTCCCCCGAGGAAGACGCCGAGGAAGCCCGCATAGAGTAAGTTTTCAACTTCATTTTT  >  NZ_CP009273/2959062‑2959237                                                                                           |                                                                                      accacGATAACGCCAATAAGGCCGCCGTGGAAAGACCTGCCGCCGTCCCAGACACGGAACAGACACAGCGGATCGGCCATAAACTGCGGGa                                                                                       >  2:849413/1‑91 (MQ=255)    acGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGTCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGGAAt                                                                                    <  1:84783/91‑1 (MQ=255)     cGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGTCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGGAAtt                                                                                   >  2:119472/1‑91 (MQ=255)       aTAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGTCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGGAATTGt                                                                                 <  1:505776/91‑1 (MQ=255)          aCGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGTCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGGAATTGTaga                                                                              >  1:547141/1‑91 (MQ=255)            gCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGTCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGGAATTGTagaac                                                                            <  2:765274/91‑1 (MQ=255)            gCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGTCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGGAATTGTagaac                                                                            <  2:620766/91‑1 (MQ=255)             ccAATCAGGCCGCCGTGGAAAGACATGCCGCCGTCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGGAATTGTagaaca                                                                           <  2:11917/91‑1 (MQ=255)                  cAGGCCGCCGTGGAAAGACATGCCGCCGTCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGGAATTGTAgaacagaaca                                                                      <  1:430955/91‑1 (MQ=255)                     gccgccGTGGAAAGACATGCCGCCGTCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGGAATTGTAGAACAGAACATaa                                                                   <  2:547141/91‑1 (MQ=255)                        gccgTGGAAAGACATGCCGCCGTCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGGAATTGTAGAACAGAACATAACCa                                                                <  1:144393/91‑1 (MQ=255)                                     aTGCCGCCGTCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGGAATTGTAGAACAGAACATAACCAATACGTCCCCCGa                                                   >  1:848309/1‑91 (MQ=255)                                          gccgTCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGGAATTGTAGAACAGAACATAACCAATACGTCCCCCGAGGAAg                                              <  1:442918/91‑1 (MQ=255)                                            cgTCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGGAATTGTAGAACAGAACATAACCAATACGTCCCCCGAGGAAGAc                                            >  1:347057/1‑91 (MQ=255)                                                   gACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGGAATTGTAGAACAGAACATAACCAATACGTCCCCCGAGGAAGACGCCGAgg                                     <  1:477513/91‑1 (MQ=255)                                                   gACACGGAACAGATACAGCGGAGCGGCCATAAACGGCGGGGAATTGTAGAACAGAACATAACCAATACGTCCCCCGAGGAAGACGCCGAgg                                     <  2:577479/91‑1 (MQ=255)                                                       cGGAACAGATACAGCGGATCGGCCATAAACTGCGGGGAATTGTAGAACAGAACATAACCAATACGTCCCCCGAGGAAGACGCCGAGGAAGc                                 >  2:27183/1‑91 (MQ=255)                                                       cGGAACAGATACAGCGGATCGGCCATAAACTGCGGGGAATTGTAGAACAGAACATAACCAATACGTCCCCCGAGGAAGACGCCGAGGAAGc                                 <  2:536087/91‑1 (MQ=255)                                                        ggAACAGATACAGCGGATCGGCCATAAACTGCGGGGAATTGTAGAACAGAACATAACCAATACGTCCCCCGAGGAAGACGCCGAGGAAGcc                                <  2:497682/91‑1 (MQ=255)                                                         gAACAGATACAGCGGATCGGCCATAAACTGCGGGGAATTGTAGAACAGAACATAACCAATACGTCCCCCGAGGAAGACGCCGAGGAAGccc                               >  1:152593/1‑91 (MQ=255)                                                                   aGCGGATCGGCCATAAACTGCGGGGAATTGTAGAACAGAACATAACCAATACGTCCCCCGAGGAAGACGCCGAGGAAGCCCGCATAGAGTa                     >  2:394387/1‑91 (MQ=255)                                                                   aGCGGATCGGCCATAAACTGCGGGGAATTGTAGAACAGAACATAACCAATACGTCCCCCGAGGAAGACGCCGAGGAAGCCCGCATAGAGTa                     >  1:788873/1‑91 (MQ=255)                                                                    gCGGATCGGCCATAAACTGCGGGGAATTGTAGAACAGAACATAACCAATACGTCCCCCGAGGAAGACGCCGAGGAAGCCCGCATAGAGTaa                    >  2:59054/1‑91 (MQ=255)                                                                                      gCGGGGAATTGTAGAACAGAACATAACCAATACGTCCCCCGAGGAAGACGCCGAGGAAGCCCGCATAGAGTAAGTTTTCAACTTCAttttt  >  2:211894/1‑91 (MQ=255)                                                                                      gCGGGGAATTGTAGAACAGAACATAACCAATACGTCCCCCGAGGAAGACGCCGAGGAAGCCCGCATAGAGTAAGTTTTCAACTTCAttttt  >  1:13653/1‑91 (MQ=255)                                                                                      gCGGGGAATTGTAGAACAGAACATAACCAATACGTCCCCCGAGGAAGACGCCGAGGAAGCCCGCATAGAGTAAGTTTTCAACTTCAttttt  >  1:443696/1‑91 (MQ=255)                                                                                           |                                                                                      ACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGTCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAGAACAGAACATAACCAATACGTCCCCCGAGGAAGACGCCGAGGAAGCCCGCATAGAGTAAGTTTTCAACTTCATTTTT  >  NZ_CP009273/2959062‑2959237

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑