Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,036,314	A→G	F65S (TTC→TCC)	yqfA ←	hemolysin III family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,036,314	0	A	G	100.0%	115.3 / NA	31	F65S (TTC→TCC)	yqfA	hemolysin III family protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (12/19);  total (12/19)

CAGGTAAATAGCGCAATGGTCAAATTTCTTCAGCCACATTTTTGCCCGTTGATGAGGAATGGCGTGATAGAGCGTCGAAGCGAGGAACAGCAGGATCATACTGCCGCCATAGAGGCTGTAGCTGGTTATCGCTGTGGCGCTGGCATTAAGATCCACCGCCTGA  >  NZ_CP009273/3036229‑3036391                                                                                      |                                                                              cAGGTAAATAGCGCAATGGTCAAATTTCTTCAGCCACATTTTTGCCCGTTGATGAGGAATGGCGTGATAGAGCGTCGAAGCGAGGGAcagc                                                                          <  2:386621/91‑1 (MQ=255) cAGGTAAATAGCGCAATGGTCAAATTTCTTCAGCCACATTTTTGCCCGTTGATGAGGAATGGCGTGATAGAGCGTCGAAGCGAGGGAcagc                                                                          <  2:845180/91‑1 (MQ=255)   ggTAAATAGCGCAATGGTCATATTTCTTCAGCCACATTTTTGCCCGTTGATGAGGAATGGCGTGATAGAGCGTCGAAGCGAGGGAcagcag                                                                        <  2:343252/91‑1 (MQ=255)   ggTAAATAGCGCAATGGTCAAATTTCTTCAGCCACATTTTTGCCCGTTGATGAGGAATGGCGTGATAGAGCGTCGAAGCGAGGGAcagcag                                                                        <  1:833249/91‑1 (MQ=255)           gcgcAATGGTCAAATTTCTTCAGCCACATTTTTGCCCGTTGATGAGGAATGGCGTGATAGAGCGTCGAAGCGAGGGACAGCAGGATCATAc                                                                >  1:579442/1‑91 (MQ=255)             gcAATGGTCAAATTTCTTCAGCCACATTTTTGCCCGTTGATGAGGAATGGCGTGATAGAGCGTCGAAGCGAGGGACAGCAGGATCATACTg                                                              >  1:68983/1‑91 (MQ=255)             gcAATGGTCAAATTTCTTCAGCCACATTTTTGCCCGTTGATGAGGAATGGCGTGATAGAGCGTCGAAGCGAGGGACAGCAGGATCATACTg                                                              >  2:302538/1‑91 (MQ=255)             gcAATGGTCAAATTTCTTCAGCCACATTTTTGCCCGTTGATGAGGAATGGCGTGATAGAGCGTCGAAGCGAGGGACAGCAGGATCATACTg                                                              >  1:492711/1‑91 (MQ=255)               aaTGGTCAAATTTCTTCAGCCACATTTTTGCCCGTTGATGAGGAATGGCGTGATAGAGCGTCGAAGCGAGGGACAGCAGGATCATACTgcc                                                            <  2:579442/91‑1 (MQ=255)                aTGGTCAAATTTCTTCAGCCACATTTTTGCCCGTTGATGAGGAATGGCGTGATAGAGCGTCGAAGCGAGGGACAGCAGGATCATACTgccg                                                           >  1:50619/1‑91 (MQ=255)                                     caTTTTTGCCCGTTGATGAGGAATGGCGTGATAGAGCGTCGAACCGAGGGACAGCAGGATCATACTGCCGCCATAGAGGCTGTAGCTGGtt                                      <  1:675507/91‑1 (MQ=255)                                                 ttGATGAGGAATGGCGTGATAGAGCGTCGAAGCGAGGGACAGCAGGATCATACTGCCGCCATAGAGGCTGTAGCTGGTTATCGCTGTGgcg                          >  1:412949/1‑91 (MQ=255)                                                   gatgaGGAATGGCGTGATAGAGCGTCGAAGCGAGGGACAGCAGGATCATACTGCCGCCATAGAGGCTGTAGCTGGTTATCGCTGTGGCGCt                        <  2:212071/91‑1 (MQ=255)                                                    gggaGGAATGGAAGGATAGAAGGTCGAGGCGAGGGACAGAAGGATCATCCTGCCGCCATAGAGGCTGTAGCTGGGTATTGCTGTGGCGCTg                       <  2:837411/89‑1 (MQ=255)                                                    atgaGGAATGGCGTGATAGAGCGTCGAAGCGAGGGACAGCAGGATCATACTGCCGCCATAGAGGCTGTAGCTGGTTATCGCTGTGGCGCTg                       <  1:445625/91‑1 (MQ=255)                                                        ggAATGGCGTGATAGAGCGTCGAAGCGAGGGACAGCAGGATCATACTGCCGCCATAGAGGCTGTAGCTGGTTATCGCTGTGGCGCTGGCAt                   <  1:393040/91‑1 (MQ=255)                                                           aTGGCGTGATAGAGCGTCGAAGCGAGGGACAGCAGGATCATACTGCCGCCATAGAGGCTGTAGCTGGTTATCGCTGTGGCGCTGGCATTaa                >  2:740823/1‑91 (MQ=255)                                                           aTGGCGTGATAGAGCGTCGAAGCGAGGGACAGCAGGATCATACTGCCGCCATAGAGGCTGTAGCTGGTTATCGCTGTGGCGCTGGCATTaa                >  2:818460/1‑91 (MQ=255)                                                            tGGCGTGATAGAGCGTCGAAGCGAGGGACGGCAGGATCAGACTGCCGCCATAGAGGCTGTAGGTGGTTATCGCTGTGGCGCTGGCATTAAg               <  2:752220/91‑1 (MQ=255)                                                             ggCGTGATAGAGCGTCGAAGCGAGGGACAGCAGGATCATACTGCCGCCATAGAGGCTGTAGCTGGTTATCGCTGTGGCGCTGGCATTAAGa              >  2:435689/1‑91 (MQ=255)                                                             ggCGTGATAGAGCGTCGAAGCGAGGGACAGCAGGATCATACTGCCGCCATAGAGGCTGTAGCTGGTTATCGCTGTGGCGCTGGCATTAAGa              <  1:351673/91‑1 (MQ=255)                                                                  gATAGAGCGTCGAAGCGAGGGACAGCAGGATCATACTGCCGCCATAGAGGCTGTAGCTGGtt                                      >  2:201932/1‑62 (MQ=255)                                                                  gATAGAGCGTCGAAGCGAGGGACAGCAGGATCATACTGCCGCCATAGAGGCTGTAGCTGGtt                                      <  1:201932/62‑1 (MQ=255)                                                                  gATAGAGCGTCGAAGCGAGGGACAGCAGGATCATACTGCCGCCATAGAGGCTGTAGCTGGTTATCGCTGTGGCGCTGGCATTAAGATCCAc         <  2:308104/91‑1 (MQ=255)                                                                  gATAGAGCGTCGAAGCGAGGGACAGCAGGATCATACTGCCGCCATAGAGGCTGTAGCTGGTTATCGCTGTGGCGCTGGCATTAAGATCCAc         <  2:412949/91‑1 (MQ=255)                                                                  gATAGAGCGTCGAAGCGAGGGACAGCAGGATCATACTGCCGCCATAGAGGCTGTAGCTGGTTATCGCTGTGGCGCTGGCATTAAGATCCAc         <  2:108347/91‑1 (MQ=255)                                                                  gATAGAGCGTCGAAGCGAGGGACAGCAGGATCATACTGCCGCCATAGAGGCTGTAGCTGGTTATCGCTGTGGCGCTGGCATTAAGATCCAc         <  2:105791/91‑1 (MQ=255)                                                                  gATAGAGCGTCGAAGCGAGGGACAGCAGGATCATACTGCCGCCATAGAGGCTGTAGCTGGTTATCGCTGTGGCGCTGGCATTAAGATCCAc         <  2:808073/91‑1 (MQ=255)                                                                        gCGTCGAAGCGAGGGACAGCAGGATCATACTGCCGCCATAGAGGCTGTAGCTGGTTATCGCTGTGGCGCt                        >  2:295513/1‑70 (MQ=255)                                                                        gCGTCGAAGCGAGGGACAGCAGGATCATACTGCCGCCATAGAGGCTGTAGCTGGTTATCGCTGTGGCGCt                        <  1:295513/70‑1 (MQ=255)                                                                        gCGTCGAAGCGAGGGACAGCAGGATCATACTGCCGCCATAGAGGCTGTAGCTGGTTATCGCTGTGGCGCTGGCATTAAGATCCACCGCCTg   >  1:258258/1‑91 (MQ=255)                                                                         cGTCGAAGCGAGGGACAGCAGGATCATACTGCCGCCATAGAGGCTGTAGCTGGTTATCGCTGTGGCGCTGGCATTAAGATCCACCGCCTGa  <  2:211708/91‑1 (MQ=255)                                                                                      |                                                                              CAGGTAAATAGCGCAATGGTCAAATTTCTTCAGCCACATTTTTGCCCGTTGATGAGGAATGGCGTGATAGAGCGTCGAAGCGAGGAACAGCAGGATCATACTGCCGCCATAGAGGCTGTAGCTGGTTATCGCTGTGGCGCTGGCATTAAGATCCACCGCCTGA  >  NZ_CP009273/3036229‑3036391

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑