Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,071,778	A→C	G147G (GGT→GGG)	cmtA ←	PTS mannitol transporter subunit IICB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,071,778	0	A	C	100.0%	108.6 / NA	29	G147G (GGT→GGG)	cmtA	PTS mannitol transporter subunit IICB
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (16/13);  total (16/13)

GCAGATAACCCGCATGTACCAGCGCCTCAATACACTCTTTGACGAAAGTATTGGCAATTAACACCGCCGGGCCGATAACTTCAAAACCCAGCAGACAAAGGAGCATCCCCGCGATACCTAATGAGAAGTTATTGATAACCATCTCAAAACCGGCAGGTATGCGTTTTTCCA  >  NZ_CP009273/3071693‑3071863                                                                                      |                                                                                      gCAGATAACCCGCATGTACCAGCGCCTCAATACACTCTTTGACGAAAGTATTGGCAATTAACACCGCCGGGCCGATAACTTCAAACCCcag                                                                                  <  2:763670/91‑1 (MQ=255) gCAGATAACCCGCATGTACCAGCGCCTCAATACACTCTTTGACGAAAGTATTGGCAATTAACACCGCCGGGCCGATAACTTCAAACCCcag                                                                                  <  1:410493/91‑1 (MQ=255)   aGATAACCCGCATGTACCAGCGCCTCAATACACTCTTTGACGAAAGTATTGGCAATTAACACCGCCGGGCCGATAACTTCAAACCCcagca                                                                                >  1:450075/1‑91 (MQ=255)      tAACCCGCATGTACCAGCGCCTCAATACACTCTTTGACGAAAGTATTGGCAATTAACACCGCCGGGCCGATAACTTCAAACCCCAGCAGAc                                                                             >  1:405435/1‑91 (MQ=255)         cccGCATGTACCAGCGCCTCAATACACTCTTTGACGAAAGTATTGGCAATTAACACCGCCGGGCCGATAACTTCAAACCCCAGCAGACaaa                                                                          <  1:376263/91‑1 (MQ=255)              aTGTACCAGCGCCTCAATACACTCTTTGACGAAAGTATTGGCAATTAACACCGCCGGGCCGATAACTTCAAACCCCAGCAGACAAAGGAGc                                                                     <  1:111073/91‑1 (MQ=255)                      gcgcCTCAATACACTCTTTGACGAAAGTATTGGCAATTAACACCGCCGGGCCGATAACTTCAAACCCcag                                                                                  <  1:155243/70‑1 (MQ=255)                      gcgcCTCAATACACTCTTTGACGAAAGTATTGGCAATTAACACCGCCGGGCCGATAACTTCAAACCCcag                                                                                  >  2:155243/1‑70 (MQ=255)                      gcgcCTCAATACACTCTTTGACGAAAGTATTGGCAATTAACACCGCCGGGCCGATAACTTCAAACCCCAGCAGACAAAGGAGCATCCCcgc                                                             >  1:494512/1‑91 (MQ=255)                        gcCTCAATACACTCTTTGACGAAAGTATTGGCAATTAACACCGCCGGGCCGATAACTTCAAACCCCAGCAGACAAAGGAGCATCCCCGCGa                                                           >  2:692733/1‑91 (MQ=255)                                   ctctTTGACGAAAGTATTGGCAATTAACACCGCCGGGCCGATAACTTCAAACCCCAGCAGACAAAGGAGCATCCCCGCGATACCTAATgag                                                >  1:337668/1‑91 (MQ=255)                                    tctTTGACGAAAGTATTGGCAATTAACACCGCCGGGCCGATAACTTCAAACCCCAGCAGACAAAGGAGCATCCCCGCGATACCTAATgaga                                               <  1:728217/91‑1 (MQ=255)                                    tctTTGACGAAAGTATTGGCAATTAACACCGCCGGGCCGATAACTTCAAACCCCAGCAGACAAAGGAGCATCCCCGCGATACCTAATgaga                                               <  2:11293/91‑1 (MQ=255)                                    tctTTGACGAAAGTATTGGCAATTAACACCGCCGGGCCGATAACTTCAAACCCCAGCAGACAAAGGAGCATCCCCGCGATACCTAATgaga                                               <  2:248955/91‑1 (MQ=255)                                     ctTTGACGAAAGTATTGGCAATTAACACCGCCGGGCCGATAACTTCAAACCCCAGCAGACAAAGGAGCATCCCCGCGATACCTAATGAGaa                                              >  2:502817/1‑91 (MQ=255)                                        tGACGAAAGTATTGGCAATTAACACCGCCGGGCCGATAACTTCAAACCCCAGCAGACAAAGGAGCATCCCCGCGATACCTAATGAGAAGtt                                           <  1:663232/91‑1 (MQ=255)                                        tGACGAAAGTATTGGCAATTAACACCGCCGGGCCGATAACTTCAAACCCCAGCAGACAAAGGAGCATCCCCGCGATACCTAATGAGAAGtt                                           <  1:835831/91‑1 (MQ=255)                                             aaaGTATTGGCAATTAACACCGCCGGGCCGATAACTTCAAACCCCAGCAGACAAAGGAGCATCCCCGCGATACCTAATGAGAAGt                                            <  1:154113/85‑1 (MQ=255)                                             aaaGTATTGGCAATTAACACCGCCGGGCCGATAACTTCAAACCCCAGCAGACAAAGGAGCATCCCCGCGATACCTAATGAGAAGt                                            >  2:154113/1‑85 (MQ=255)                                               aGTATTGGCAATTAACACCGCCGGGCCGATAACTTCAAACCCCAGCAGACAAAGGAGCATCCCCGCGATACCTAATGAGAAGTTATTGATa                                    >  2:593178/1‑91 (MQ=255)                                                       cAATTAACACCGCCGGGCCGATAACTTCAAACCCCAGCAGACAAAGGAGCATCCCCGCGATACCTAATGAGAAGTTATTGATAACCAtctc                            >  2:46659/1‑91 (MQ=255)                                                          ttAACACCGCCGGGCCGATAACTTCAAACCCCAGCAGACAAAGGAGCATCCCCGCGATACCTAATGAGAAGTTATTGATAACCATCTCaaa                         >  2:52067/1‑91 (MQ=255)                                                          ttAACACCGCCGGGCCGATAACTTCAAACCCCAGCAGACAAAGGAGCATCCCCGCGATACCTAATGAGAAGTTATTGATAACCATCTCaaa                         >  1:557321/1‑91 (MQ=255)                                                               acCGCCGGGCCGATAACTTCAAACCCCAGCAGACAAAGGAGCATCCCCGCGATACCTAATGAGAAGTTATTGATAACCATCTCAAAACCgg                    >  1:704797/1‑91 (MQ=255)                                                                 cgccgGGCCGATAACTTCAAACCCCAGCAGACAAAGGAGCATCCCCGCGATACCTAATGAGAAGTTATTGATAACCATCTCAAAACCGGCa                  >  2:156960/1‑91 (MQ=255)                                                                 cgccgGGCCGATAACTTCAAACCCCAGCAGACAAAGGAGCATCCCCGCGATACCTAATGAGAAGTTATTGATAACCATCTCAAAACCGGCa                  <  2:173749/91‑1 (MQ=255)                                                                     gggCCGATAACTTCAAACCCCAGCAGACAAAGGAGCATCCCCGCGATACCTAATGAGAAGTTATTGATAACCATCTCAAAACCGGCAGGTa              >  2:89996/1‑91 (MQ=255)                                                                      ggCCGATAACTTCAAACCCCAGCAGACAAAGGAGCATCCCCGCGATACCTAATGAGAAGTTATTGATAACCATCTCAAAACCGGCAGGTAt             <  2:747352/91‑1 (MQ=255)                                                                                 tCAAACCCCAGCAGACAAAGGAGCATCCCCGCGATACCTAATGAGAAGTTATTGATAACCATCTCAAAACCGGCAGGTATGCGTTTTTCCa  >  1:717347/1‑91 (MQ=255)                                                                                      |                                                                                      GCAGATAACCCGCATGTACCAGCGCCTCAATACACTCTTTGACGAAAGTATTGGCAATTAACACCGCCGGGCCGATAACTTCAAAACCCAGCAGACAAAGGAGCATCCCCGCGATACCTAATGAGAAGTTATTGATAACCATCTCAAAACCGGCAGGTATGCGTTTTTCCA  >  NZ_CP009273/3071693‑3071863

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑