Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,075,672	A→T	H134L (CAC→CTC)	loiP →	metalloprotease LoiP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,075,672	0	A	T	100.0%	95.6 / NA	25	H134L (CAC→CTC)	loiP	metalloprotease LoiP
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (10/15);  total (10/15)

CTAACGGCTGTATCCGCGTCTATAGCGGGCTGATGGATATGATGACGGATAACGAAGTCGAAGCGGTGATCGGTCACGAAATGGGGCACGTGGCGTTAGGCCATGTGAAAAAAGGAATGCAGGTGGCACTTGGTACAAATGCCGTGCGAGTAGCTGCGGCCTCTGCGGGCGGG  >  NZ_CP009273/3075585‑3075757                                                                                        |                                                                                      cTAACGGCTGTATCCGCGTCTATAGCGGGCTGATGGATATGATGACGGATAACGAAGTCGAAGCGGTGATCGGTCACGAAATGGGGCTCGt                                                                                    <  1:692263/91‑1 (MQ=255) cTAACGGCTGTATCCGCGTCTATAGCGGGCTGATGGATATGATGACGGATAACGAAGTCGAAGCGGTGATCGGTCACGAAATGGGGCTCGt                                                                                    <  2:389878/91‑1 (MQ=255) cTAACGGCTGTATCCGCGTCTATAGCGGGCTGATGGATATGATGACGGATAACGAAGTCGAAGCGGTGATCGGTCACGAAATGGGGCTCGt                                                                                    <  2:523234/91‑1 (MQ=255) cTAACGGCTGTATCCGCGTCTATAGCGGGCTGATGGATATGATGACGGATAACGAAGTCGAAGCGGTGATCGGTCACGAAATGGGGCTCGt                                                                                    <  1:505716/91‑1 (MQ=255)       gCTGTATCCGCGTCTATAGCGGGCTGATGGATATGATGACGGATAACGAAGTCGAAGCGGTGATCGGTCACGAAATGGGGCTCGTGGCGtt                                                                              <  2:634356/91‑1 (MQ=255)                 agTCTATAGCGGGCTGATGGATATGATGACGGATAAAGCAGTCGAAGCGGTGATCGGTCACGAAATGGGGCTCGTGGCGGTAGGCCAtgtg                                                                    >  2:235469/2‑91 (MQ=255)                       taGCGGGCTGATGGATATGATGACGGATAACGAAGTCGAAGCGGTGATCGGTCACGAAATGGGGCTCGTGGCGTTAGGCCATGTGaaaaaa                                                              <  1:28704/91‑1 (MQ=255)                       taGCGGGCTGATGGATATGATGACGGATAACGAAGTCGAAGCGGTGATCGGTCACGAAATGGGGCTCGTGGCGTTAGGCCATGTGaaaaaa                                                              <  1:793462/91‑1 (MQ=255)                           gggCTGATGGATATGATGACGGATAACGAAGTCGAAGCGGTGATCGGTCACGAAATGGGGCTCGTGGCGTTAGGCCATGTGAAAAAAGGaa                                                          >  2:577812/1‑91 (MQ=255)                                gATGGATATGATGACGGATAACGAAGTCGAAGCGGTGATCGGTCACGAAATGGGGCTCGTGGCGTTAGGCCATGTGAAAAAAGGAATGCAg                                                     >  1:195820/1‑91 (MQ=255)                                          atgaCGGATAACGAAGTCGAAGCGGTGATCGGTCACGAAATGGGGCTCGTGGCGTTAGGCCATGTGAAAAAAGGAATGCAGGTGGCACTTg                                           <  1:707359/91‑1 (MQ=255)                                          atgaCGGATAACGAAGTCGAAGCGGTGATCGGTCACGAAATGGGGCTCGTGGCGTTAGGCCATGTGAAAAAAGGAATGCAGGTGGCACTTg                                           <  2:237229/91‑1 (MQ=255)                                          atgaCGGATAACGAAGTCGAAGCGGTGATCGGTCACGAAATGGGGCTCGTGGCGTTAGGCCATGTGAAAAAAGGAATGCAGGTGGCACTTg                                           >  1:113420/1‑91 (MQ=255)                                          ataaCGGATAACGAAGTCGAAGCGGTGATCGGTCACGAAATGGGGCTCGTGGCGTTAGGCCATGTGAAAAAAGGAACGCAGGTGGCACTTg                                           <  1:745916/88‑1 (MQ=255)                                              cGGATAACGAAGTCGAAGCGGTGATCGGTCACGAAATGGGGCTCGTGGCGTTAGGCCATGTGAAAAAAGGAATGCAGGTGGCACTTGGTAc                                       >  2:550668/1‑91 (MQ=255)                                                  tAACGAAGTCGAAGCGGTGATCGGTCACGAAATGGGGCTCGTGGCGTTAGGCCAtg                                                                      >  1:457347/1‑56 (MQ=255)                                                  tAACGAAGTCGAAGCGGTGATCGGTCACGAAATGGGGCTCGTGGCGTTAGGCCAtg                                                                      <  2:457347/56‑1 (MQ=255)                                                   aaCGAAGTCGAAGCGGTGATCGGTCACGAAATGGGGCTCGTGGCGTTAGGCCATGTGAAAAAAGGAATGCAGGTGGCACTTGGTACAAATg                                  >  1:392364/1‑91 (MQ=255)                                                         gTCGAAGCGGTGATCGGTCACGAAATGGGGCTCGTGGCGTTAGGCCATGTGAAAAAAGGAATGCAGGTGGCACTTg                                           <  1:120417/76‑1 (MQ=255)                                                         gTCGAAGCGGTGATCGGTCACGAAATGGGGCTCGTGGCGTTAGGCCATGTGAAAAAAGGAATGCAGGTGGCACTTg                                           >  2:191419/1‑76 (MQ=255)                                                         gTCGAAGCGGTGATCGGTCACGAAATGGGGCTCGTGGCGTTAGGCCATGTGAAAAAAGGAATGCAGGTGGCACTTg                                           >  2:120417/1‑76 (MQ=255)                                                         gTCGAAGCGGTGATCGGTCACGAAATGGGGCTCGTGGCGTTAGGCCATGTGAAAAAAGGAATGCAGGTGGCACTTg                                           <  1:191419/76‑1 (MQ=255)                                                         gTCGAAGCGGTGATCGGTCACGAAATGGGGCTCGTGGCGTTAGGCCATGTGAAAAAAGGAATGCAGGTGGCACTTGGTACAAATGCCGTGc                            >  2:641011/1‑91 (MQ=255)                                                                  gTGATCGGTCACGAAATGGGGCTCGTGGCGTTAGGCCATGTGAAAAAAGGAATGCAGGTGGCACTTGGTACAAATGCCGTGCGAGTAGCTg                   <  2:161476/91‑1 (MQ=255)                                                                     aTCGGTCACGAAATGGGGCTCGTGGCGTTAGGCCATGTGAAAAAAGGAATGCAGGTGGCACTTGGTACAAATGCCGTGCGAGTAGCTGCgg                <  1:647857/91‑1 (MQ=255)                                                                                   ggggCTCGTGGCGTTAGGCCATGTGAAAAAAGGAATGCAGGTGGCACTTGGTACAAATGCCGTGCGAGTAGCTGCGGCCTCTGcgggcggg  >  2:763583/1‑91 (MQ=255)                                                                                        |                                                                                      CTAACGGCTGTATCCGCGTCTATAGCGGGCTGATGGATATGATGACGGATAACGAAGTCGAAGCGGTGATCGGTCACGAAATGGGGCACGTGGCGTTAGGCCATGTGAAAAAAGGAATGCAGGTGGCACTTGGTACAAATGCCGTGCGAGTAGCTGCGGCCTCTGCGGGCGGG  >  NZ_CP009273/3075585‑3075757

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑