Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,148,355	A→G	S56P (TCC→CCC)	yqhC ←	DNA‑binding transcriptional regulator YqhC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,148,355	0	A	G	100.0%	103.2 / NA	28	S56P (TCC→CCC)	yqhC	DNA‑binding transcriptional regulator YqhC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (11/17);  total (11/17)

CAAACGGCAACGGCACCGTCAGCAGCAGGTATTCATTGGCATCATAACGAAACACGCGTTCATTGATATAACCGATTTTATGCCCGGAAAAGAGAATTATGATGCCAGGCTCGTACATCACCGGTGTACGTGCGAAAGGCGTCTCGCCATACAACAAACGCACATCGGGCAAC  >  NZ_CP009273/3148268‑3148440                                                                                        |                                                                                      cAAACGGCAACGGCACCGTCAGCAGCAGGTATTCATTGGCATCATAACGAAACACGCGTTCATTGATATAACCGATTTTATGCCCGGgaaa                                                                                    <  1:268576/91‑1 (MQ=255)  aaaCGGCAACGGCACCGTCAGCAGCAGGTATTCATTGGCATCATAACGAAACACGCGTTCATTGATATAACCGATTTTATGCCCGGGAAag                                                                                   <  2:38862/91‑1 (MQ=255)  aaaCGGCAACGGCACCGTCAGCAGCAGGTATTCATTGGCATCATAACGAAACACGCGTTCATTGATATAACCGATTTTATGCCCGGGAAag                                                                                   >  1:650427/1‑91 (MQ=255)  aaaCGGCAACGGCACCGTCAGCAGCAGGTATTCATTGGCATCATAACGAAACACGCGTTCATTGATATAACCGATTTTATGCCCGGGAAag                                                                                   <  1:657378/91‑1 (MQ=255)          acggcaCCGTCAGCAGCAGGTATTCATTGGCATCATAACGAAACACGCGTTCATTGATATAACCGATTTTATGCCCGGGAAAGAGAATTat                                                                           <  1:604737/91‑1 (MQ=255)           cggcaCCGTCAGCAGCAGGAATTCATTGGCATCATAACGAAACACGCGTTCATTGATATAACCGATTTTATGCCCGGGAAAGAGAATTatg                                                                          >  2:193713/1‑91 (MQ=255)                ccGTCAGCAGCAGGTATTCATTGGCATCATAACGAAACACGCGTTCATTGATATAACCGATTTTATGCCCGGGAAAGAGAATTATGATGcc                                                                     <  1:494236/91‑1 (MQ=255)                  gTCAGCAGCAGGTATTCATTGGCATCATAACGAAACACGCGTTCATTGATATAACCGATTTTATGCCCGGGAAAGAGAATTATGATGCCAg                                                                   <  1:242955/91‑1 (MQ=255)                  gTCAGCAGCAGGTATTCATTGGCATCATAACGAAACACGCGTTCATTGATATAACCGATTTTATGCCCGGGAAAGAGAATTATGATGCCAg                                                                   >  1:421533/1‑91 (MQ=255)                  gTCAGCAGCAGGTATTCATTGGCATCATAACGAAACACGCGTTCATTGAGATAACCGATTTTATGCCCGGGAAAGAGAATTATGATGCCAg                                                                   <  2:317036/91‑1 (MQ=255)                      gcagcagGTATTCATTGGCATCATAACGAAACACGCGTTCATTGATATAACCGATTTTATGCCCGGGAAAGAGAATTATGATGCCAGGCTc                                                               <  1:817053/91‑1 (MQ=255)                        agcagGTATTCATTGGCATCATAACGAAACACGCGTTCATTGATATAACCGATTTTATGCCCGGGAAAGAGAATTATGATGCCAGGCTCGt                                                             <  1:757835/91‑1 (MQ=255)                            ggTATTCATTGGCATCATAACGAAACACGCGTTCATTGATATAACCGATTTTATGCCCGGGAAAGAGAATTATGATGCCAGGCTCGTACAt                                                         >  2:452538/1‑91 (MQ=255)                             gTATTCATTGGCATCATAACGAAACACGCGTTCATTGATATAACCGATTTTATGCCCGGGAAAGAGAATTATGATGCCAGGCTCGTACATc                                                        <  2:530176/91‑1 (MQ=255)                                ttCATTGGCATCATAACGAAACACGCGTTCATTGATATAACCGATTTTATGCCCGGGAAAGAGAATTATGATGCCAGGCTCATACATCAcc                                                     <  2:479443/91‑1 (MQ=255)                                    ttGGCATCATAACGAAACACGCGTTCATTGATATAACCGATTTTATGCCCGGGAAAGAGAATTATGATGCCAGGCTCGTACATCACCGgtg                                                 >  1:187804/1‑91 (MQ=255)                                       gCATCATAACGAAACACGCGTTCATTGATATAACCGATTTTATGCCCGGGAAAGAGAATTATGATGCCAGGCTCGTACATCACCGGTGTAc                                              >  1:342794/1‑91 (MQ=255)                                                       cgcgTTCATTGATATAACCGATTTTATGCCCGGGAAAGAGAATTATGATGCCAGGCTCGTACATCACCGGTGTACGTGCGAAAGGCGtctc                              <  2:414536/91‑1 (MQ=255)                                                                 gATATAACCGATTTTATGCCCGGGAAAGAGAATTATGATGCCAGGCTCGTACATCACCGGTGTACGTGCGAAAGGCGTCTCGCCATacaac                    <  1:445685/91‑1 (MQ=255)                                                                 gATATAACCGATTTTATGCCCGGGAAAGAGAATTATGATGCCAGGCTCGTACATCACCGGTGTACGTGCGAAAGGCGTCTCGCCATacaac                    <  2:386234/91‑1 (MQ=255)                                                                       aCCGATTTTATGCCCGGGAAAGAGAATTATGATGCCAGGCTCGTACATCACCGGTGTACGTGCGAAAGGCGTCTCGCCATACAACAAACGc              >  1:624192/1‑91 (MQ=255)                                                                       aCCGATTTTATGCCCGGGAAAGAGAATTATGATGCCAGGCTCGTACATCACCGGTGTACGTGCGAAAGGCGTCTCGCCATACAACAAACGc              >  1:360078/1‑91 (MQ=255)                                                                         cGATTTTATGCCCGGGAAAGAGAATTATGATGCCAGGCTCGTACATCACCGGTGTACGTGCGAAAGGCGTCTCGCCATACAACAAACGcac            <  2:389/91‑1 (MQ=255)                                                                           aTTTTATGCCCGGGAAAGAGAATTATGATGCCAGGCTCGTACATCACCGGTGTACGTGCGAAAGGCGTCTCGCCATACAACAAACGCACAt          >  2:822439/1‑91 (MQ=255)                                                                           aTTTTATGCCCGGGAAAGAGAATTATGATGCCAGGCTCGTACATCACCGGTGTACGTGCGAAAGGCGTCTCGCCATACAACAAACGCACAt          >  1:201722/1‑91 (MQ=255)                                                                           aTTTTATGCCCGGGAAAGAGAATTATGATGCCAGGCTCGTACATCACCGGTGTACGTGCGAAAGGCGTCTCGCAATACAACAAACGCACAt          >  2:831841/1‑91 (MQ=255)                                                                                  gCCCGGGAAAGAGAATTATGATGCCAGGCTCGTACATCACCGGTGTACGTGCGAAAGGCGTCTCGCCATACAACAAACGCACATCGGGCaa   <  2:187804/91‑1 (MQ=255)                                                                                   cccGGGAAAGAGAATTATGATGCCAGGCTCGTACATCACCGGTGTACGTGCGAAAGGCGTCTCGCCATACAACAAACGCACATCGGGCAAc  <  2:650427/91‑1 (MQ=255)                                                                                   cccGGGAAAGAGAATTATGATGCCAGGCTCGTACATCACCGGTGTACGTGCGAAAGGCGTCTCGCCATACAACAAACGCACATCGGGCAAc  <  1:77961/91‑1 (MQ=255)                                                                                        |                                                                                      CAAACGGCAACGGCACCGTCAGCAGCAGGTATTCATTGGCATCATAACGAAACACGCGTTCATTGATATAACCGATTTTATGCCCGGAAAAGAGAATTATGATGCCAGGCTCGTACATCACCGGTGTACGTGCGAAAGGCGTCTCGCCATACAACAAACGCACATCGGGCAAC  >  NZ_CP009273/3148268‑3148440

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑