Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,155,922	A→G	V36A (GTT→GCT)	ftsP ←	cell division protein FtsP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,155,922	0	A	G	100.0%	122.0 / NA	33	V36A (GTT→GCT)	ftsP	cell division protein FtsP
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (20/13);  total (20/13)

GGCGTAAATGACCAGTGCGCACGTTGTACAGTCATAAACAGCGGTTGCCCACGGCGAGATTCAAGTAGCGGCGGAACGGGTAGCGGTTGCTGTTGCCCGGCTGCGCTGGCCTTCAGGGGAACAGCGCCTGCACAAAGTGCAATCCCCGATGCCTGAATGAAC  >  NZ_CP009273/3155847‑3156008                                                                            |                                                                                       ggCGTAAATGACCAGTGCGCAGGTTGTACCGTCATAACCAGCGGCTGCCCACGGCGAGATTCAAGTACCGGCGGTGGGGGTGGCGGTTGCt                                                                         >  2:423562/1‑91 (MQ=255)      aaaTGACCAGTGCGCACGTTGTACAGTCATAAACAGCGGTTGCCCACGGCGAGATTCAAGTAGCGGCGGAGCGGGTAGGGGTTGCTGTTGc                                                                    >  2:666457/1‑91 (MQ=255)      aaaTGACCAGTGCGCACGTTGTACAGTCATAAACAGCGGTTGCCCACGGCGAGATTCAAGTAGCGGCGGAGCGGGTAGCGGTTGCTGt                                                                       <  1:786224/88‑1 (MQ=255)      aaaTGACCAGTGCGCACGTTGTACAGTCATAAACAGCGGTTGCCCACGGCGAGATTCAAGTAGCGGCGGAGCGGGTAGCGGTTGCTGt                                                                       >  2:786224/1‑88 (MQ=255)      aaaTGACCAGTGCGCACGTTGTACAGTCATAAACAGCGGTTGCCCACGGCGAGATTCAAGTAGCGGCGGAGCGGGTAGCGGTTGCTGTTGc                                                                    >  1:585153/1‑91 (MQ=255)               gTGCGCACGTTGTACAGTCATAAACAGCGGTTGCCCACGGCGAGATTCAAGTAGCGGCGGAGCGGGTAGCGGTTGCTGTTGCCCGGCTgcg                                                           >  2:530480/1‑91 (MQ=255)                tGCGCACGTTGTACAGTCATAAACAGCGGTTGCCCACGGCGAGATTCAAGTAGCGGCGGAGCGGGTAGCGGTTGCTGTTGCCCGGCTgcgc                                                          <  1:489768/91‑1 (MQ=255)                 gcgcACGTTGTACAGTCATAAACAGCGGTTGCCCACGGCGAGATTCAAGTAGCGGCGGAGCGGGTAGCGGTTGCTGTTGCCCGGCTGCGCt                                                         >  1:403021/1‑91 (MQ=255)                  cgcACGTTGTACAGTCATAAACAGCGGTTGCCCACGGCGAGATTCAAGTAGCGGCGGAGCGGGTAGCGGTTGCTGTTGCCCGGCTGCGCTg                                                        <  1:170180/91‑1 (MQ=255)                      cGTTGTACAGTCATAAACAGCGGTTGCCCACGGCGAGATTCAAGTAGCGGCGGAGCGGGTAGCGGTTGCTGTTGCCCGGCTGCGCTGGCCt                                                    <  2:84831/91‑1 (MQ=255)                        ttGTACAGTCATAAACAGCGGTTGCCCACGGCGAGATTCAAGTAGCGGCGGAGCGGGTAGCGGTTGCTGTTGCCCGGCTGCGCTGGCCTTc                                                  <  2:849834/91‑1 (MQ=255)                         tGTACAGTCATAAACAGCGGTTGCCCACGGCGAGATTCAAGTAGCGGCGGAGCGGGTAGCGGTTGCTGTTGCCCGGCTGCGCTGGCCTTCa                                                 >  1:659899/1‑91 (MQ=255)                         tGTACAGTCATAAACAGCGGTTGCCCACGGCGAGATTCAAGTAGCGGCGGAGCGGGTAGCGGTTGCTGTTGCCCGGCTGCGCTGGCCTTCa                                                 >  1:684232/1‑91 (MQ=255)                         tGTACAGTCATAAACAGCGGTTGCCCACGGCGAGATTCAAGTAGCGGCGGAGCGGGTAGCGGTTGCTGTTGCCCGGCTGCGCTGGCCTTCa                                                 >  2:838897/1‑91 (MQ=255)                           tACAGTCATAAACAGCGGTTGCCCACGGCGAGATTCAAGTAGCGGCGGAGCGGGTAGCGGTTGCTGTTGCCCGGCTGCGCTGGCCTTCAgg                                               <  2:683601/91‑1 (MQ=255)                           tACAGTCATAAACAGCGGTTGCCCACGGCGAGATTCAAGTAGCGGCGGAGCGGGTAGCGGTTGCTGTTGCCCGGCTGCGCTGGCCTTCAgg                                               <  1:338480/91‑1 (MQ=255)                                tCATAAACAGCGGTTGCCCACGGCGAGATTCAAGTAGCGGCGGAGCGGGTAGCGGTTGCTGTTGCCCGGCTGCGCTGGCCTTCAGGGGAAc                                          >  1:578792/1‑91 (MQ=255)                                 cATAAACAGCGGTTGCCCACGGCGAGATTCAAGTAGCGGCGGAGCGGGTAGCGGTTGCTGTTGCCCGGCTGCGCTGGCCTTCAGGGGAACa                                         <  2:439409/91‑1 (MQ=255)                                 cATAAACAGCGGTTGCCCACGGCGAGATTCAAGTAGCGGCGGAGCGGGTAGCGGTTGCTGTTGCCCGGCTGCGCTGGCCTTCAGGGGAACa                                         >  1:160382/1‑91 (MQ=255)                                  aTAAACAGCGGTTGCCCACGGCGAGATTCAAGTAGCGGCGGAGCGGGTAGCGGTTGCTGTTGCCCGGCTGCGCTgg                                                       <  2:634053/76‑1 (MQ=255)                                  aTAAACAGCGGTTGCCCACGGCGAGATTCAAGTAGCGGCGGAGCGGGTAGCGGTTGCTGTTGCCCGGCTGCGCTgg                                                       >  1:634053/1‑76 (MQ=255)                                  aTAAACAGCGGTTGCCCACGGCGAGATTCAAGTAGCGGCGGAGCGGGTAGCGGTTGCTGTTGCCCGGCTGCGCTg                                                        <  2:227864/75‑1 (MQ=255)                                  aTAAACAGCGGTTGCCCACGGCGAGATTCAAGTAGCGGCGGAGCGGGTAGCGGTTGCTGTTGCCCGGCTGCGCTg                                                        >  1:227864/1‑75 (MQ=255)                                  aTAAACAGCGGTTGCCCACGGCGAGATTCAAGTAGCGGCGGAGCGGGTAGCGGTTGCTGTTGCCCGGCTGCGCTGGCCTTCAGGGGAACAg                                        >  1:791871/1‑91 (MQ=255)                                    aaaCAGCGGTTGCCCACGGCGAGATTCAAGTAGCGGCGGAGCGGGTAGCGGTTGCTGTTGCCCGGCTGCGCTGGCCTTCAGGGGAACAgcg                                      >  2:552280/1‑91 (MQ=255)                                    aaaCAGCGGTTGCCCACGGCGAGATTCAAGTAGCGGCGGAGCGGGTAGCGGTTGCTGTTGCCCGGCTGCGCTGGCCTTCAGGGGAACAgcg                                      >  2:844775/1‑91 (MQ=255)                                         gCGGTTGCCCACGGCGAGATTCAAGTAGCGGCGGAGCGGGTAGCGGTTGCTGTTGCCCGGCTGCGCt                                                         >  2:454530/1‑67 (MQ=255)                                         gCGGTTGCCCACGGCGAGATTCAAGTAGCGGCGGAGCGGGTAGCGGTTGCTGTTGCCCGGCTGCGCt                                                         <  1:454530/67‑1 (MQ=255)                                         gCGGTTGCCCACGGCGAGATTCAAGTAGCGGCGGAGCGGGTAGCGGTTGCTGTTGCCCGGCTGCGCTGGCCTTCAGGGGAACAGCGCCTGc                                 >  1:853629/1‑91 (MQ=255)                                         gCGGTTGCCCACGGCGAGATTCAAGTAGCGGCGGAGCGGGTAGCGGTTGCTGTTGCCCGGCTGCGCTGGCCTTCAGGGGAACAGCGCCTGc                                 >  1:196706/1‑91 (MQ=255)                                             ttGCCCACGGCGAGATTCAAGTAGCGGCGGAGCGGGTAGCGGTTGCTGTTGCCCGGCTGCGCTGGCCTTCAGGGGAACAGCGCCTGCACaa                             <  2:578792/91‑1 (MQ=255)                                                                    gcggcgGAGCGGGTAGCGGTTGCTGTTGCCCGGCTGCGCTGGCCTTCAGGGGAACAGCGCCTGCACAAAGTGCAATCCCCGATGCCtgaat      >  1:751942/1‑91 (MQ=255)                                                                     cggcggAGCGGGTAGCGGTTGCTGTTGCCCGGCTGCGCTGGCCTTCAGGGGAACAGCGCCTGCACAAAGTGCAATCCCCGATGCCtgaatg     >  1:115753/1‑91 (MQ=255)                                                                        cggAGCGGGTAGCGGTTGCTGTTGCCCGGCTGCGCTGGCCTTCAGGGGAACAGCGCCTGCACAAAGTGCAATCCCCGATGCCTGAATGAAc  <  2:337860/91‑1 (MQ=255)                                                                            |                                                                                       GGCGTAAATGACCAGTGCGCACGTTGTACAGTCATAAACAGCGGTTGCCCACGGCGAGATTCAAGTAGCGGCGGAACGGGTAGCGGTTGCTGTTGCCCGGCTGCGCTGGCCTTCAGGGGAACAGCGCCTGCACAAAGTGCAATCCCCGATGCCTGAATGAAC  >  NZ_CP009273/3155847‑3156008

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑