Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,193,900	A→G	L142P (CTC→CCC)	ygiF ←	inorganic triphosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,193,900	0	A	G	100.0%	96.8 / NA	27	L142P (CTC→CCC)	ygiF	inorganic triphosphatase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (17/10);  total (17/10)

CGCGCGCGTGTCGCCGCTAAGCAGTTCCAGTTCCAGCTCACAGATAGGTTCAGCAAATTCACCCGCTTTCACTTCCCCCTGGTCGAGGGCGATTTCAATTTGGCTACCATCGACCGCCACCAGCCATTTTTCGCGATAAAAATCGGTGCTGAACAGCGGCTGCACGCGGGAG  >  NZ_CP009273/3193815‑3193986                                                                                      |                                                                                       cgcgcgcgTGTCGCCGCTAAGCAGTTCCAGTTCCAGCTCACAGATAGGTTCAGCAAATTCACCCGCTTTCACTTCCCCCTGGTCGGGGGCg                                                                                   >  1:151951/1‑91 (MQ=255) cgcgcgcgTGTCGCCGCTAAGCAGTTCCAGTTCCAGCTCACAGATAGGTTCAGCAAATTCACCCGCTTTCACTTCCCCCTGGTCGGGGGCg                                                                                   >  2:87930/1‑91 (MQ=255) cgcgcgcgTGTCGCCGCTAAGCAGTTCCAGTTCCAGCTCACAGATAGGTTCAGCAAATTCACCCGCTTTCACTTCCCCCTGGTCGGGGGCg                                                                                   >  1:842658/1‑91 (MQ=255) cgcgcgcgTGTCGCCGCTAAGCAGTTCCAGTTCCAGCTCACAGATAGGTTCAGCAAATTCACCCGCTTTCACTTCCCCCTGGTCGGGGGCg                                                                                   <  2:606776/91‑1 (MQ=255)  gcgcgcgTGTCGCCGCTAAGCAGTTCCAGTTCCAGCTCACAGATAGGTTCAGCAAATTCACCCGCTTTCACTTCCCCCTGGTCGGGGGCGa                                                                                  >  1:333001/1‑91 (MQ=255)  gcgcgcgTGTCGCCGCTAAGCAGTTCCAGTTCCAGCTCACAGATAGGTTCAGCAAATTCACCCGCTTTCACTTCCCCCTGGTCGGGGGCGa                                                                                  >  1:635160/1‑91 (MQ=255)   cgcgcgTGTCGCCGCTAAGCAGTTCCAGTTCCAGCTCACAGATAGGTTCAGCAAATTCACCCGCTTTCACTTCCCCCTGGTCGGGGGCGAt                                                                                 >  2:241730/1‑91 (MQ=255)    gcgcgTGTCGCCGCTAAGCAGTTCCAGTTCCAGCTCACAGATAGGTTCAGCAAATTCACCCGCTTTCACTTCCCCCTGGTCGGGGGCGAtt                                                                                <  1:824787/91‑1 (MQ=255)          gtCGCCGCTAAGCAGTTCCAGTTCCAGCTCACAGATAGGTTCAGCAAATTCACCCGCTTTCACTTCCCCCTGGTCGGGGGCGATTTCAAtt                                                                          <  1:87930/91‑1 (MQ=255)          gtCGCCGCTAAGCAGTTCCAGTTCCAGCTCACAGATAGGTTCAGCAAATTCACCCGCTTTCACTTCCCCCTGGTCGGGGGCGATTTCAAtt                                                                          <  2:622546/91‑1 (MQ=255)            cgccgcTAAGCAGTTCCAGTTCCAGCTCACAGATAGGTTCAGCAAATTCACCCGCTTTCACTTCCCCCTGGTCGGGGGCGATTTCAATTTg                                                                        >  2:819123/1‑91 (MQ=255)                   aaGCAGTTCCAGTTCCAGCTCACAGATAGGTTCAGCAAATTCACCCGCTTTCACTTCCCCCTGGTCGGGGGCGATTTCAATTTGGCTACCa                                                                 >  2:830228/1‑91 (MQ=255)                         ttccagttccagCTCACAGATAGGTTCAGCAAATTCACCCGCTTTCACTTCCCCCTGGTCGGGGGCGATTTCAATTTGGCTACCATCGAcc                                                           <  2:668416/91‑1 (MQ=255)                               ttccagCTCACAGATAGGTTCAGCAAATTCACCCGCTTTCACTTCCCCCTGGTCGGGGGCGATTTCAATTTGGCTACCATCGACCGccacc                                                     <  1:152728/91‑1 (MQ=255)                                  cagCTCACAGATAGGTTCAGCAAATTCACCCGCTTTCACTTCCCCCTGGTCGGGGGCGATTTCAATTTGGCTACCATCGACCGCCACCAGc                                                  >  2:651754/1‑91 (MQ=255)                                   agCTCACAGATAGGTTCAGCAAATTCACCCGCTTTCACTTCCCCCTGGTCGGGGGCGATTTCAATTTGGCTACCATCGACCGCCACCAGcc                                                 >  2:47936/1‑91 (MQ=255)                                    gCTCACAGATAGGTTCAGCAAATTCACCCGCTTTCACTTCCCCCTGGTCGGGGGCGATTTCAATTTGGCTACCATCGACCGCCACCAGCCa                                                >  2:675880/1‑91 (MQ=255)                                              aGGTTCAGCAAATTCACCCGCTTTCACTTCCCCCTGGTCGGGGGCGATTTCAATTTGGCTACCATCGACCGCCACCAGCCATTTTTCGCGa                                      >  2:352315/1‑91 (MQ=255)                                                   cAGCAAATTCACCCGCTTTCACTTCCCCCTGGTCGGGGGCGATGTCAATTTGGCTACCATCGACCGCCACCAGCCATTTTTCGCGATaaaa                                 >  1:843417/1‑91 (MQ=255)                                                     gCAAATTCACCCGCTTTCACTTCCCCCTGGTCGGGGGCGATTTCAATTTGGCTACCATCGACCGCCACCAGCCATTTTTCGCGATAAAAAt                               >  2:334079/1‑91 (MQ=255)                                                      gaaaTTCGCCCGCTTTCCCTCCCCCCTGGTCGGGGGCGTTTTCAATTTGGCTACCAGCGACCGCCACCAGCCATTTTTCGCGATAAAAATc                              <  2:139412/90‑1 (MQ=255)                                                      cAAATTCACCCGCTTTCACTTCCCCCTGGTCGGGGGCGATTTCAATTTGGCTACCATCGACCGCCACCAGCCATTTTTCGCGATAAAAATc                              <  1:645583/91‑1 (MQ=255)                                                          ttCACCCGCTTTCACTTCCCCCTGGTCGGGGGCGATTTCAATTTGGCTACCATCGACCGCCACCAGCCATTTTTCGCGATAAAAATCGGTg                          >  2:620425/1‑91 (MQ=255)                                                          ttCACCCGCTTTCACTTCCCCCTGGTCGGGGGCGATTTCAATTTGGCTACCATCGACCGCCACCAGCCATTTTTCGCGATAAAAATCGGTg                          >  2:769814/1‑91 (MQ=255)                                                           tCACCCGCTTTCACTTCCCCCTGGTCGGGGGCGATTTCAATTTGGCTACCATCGACCGCCACCAGCCATTTTTCGCGATAAAAATCGGTGc                         <  1:352315/91‑1 (MQ=255)                                                            cACCCGCTTTCACTTCCCCCTGGTCGGGGGCGATTTCAATTTGGCTACCATCGACCGCCACCAGCCATTTTTCGCGATAAAAATCGGTGCt                        <  2:302377/91‑1 (MQ=255)                                                                                tGGTCGGGGGCGATTTCAATTTGGCTACCATCGACCGCCACCAGCCATTTTTCGCGATAAAAATCGGTGCTGAACAGCGGCTGCACGCggg    >  2:32355/1‑91 (MQ=255)                                                                                  gTCGGGGGCGATTTCAATTTGGCTACCATCGACCGCCACCAGCCATTTTTCGCGATAAAAATCGGTGCTGAACAGCGGCTGCACGCGGGAg  <  1:675880/91‑1 (MQ=255)                                                                                      |                                                                                       CGCGCGCGTGTCGCCGCTAAGCAGTTCCAGTTCCAGCTCACAGATAGGTTCAGCAAATTCACCCGCTTTCACTTCCCCCTGGTCGAGGGCGATTTCAATTTGGCTACCATCGACCGCCACCAGCCATTTTTCGCGATAAAAATCGGTGCTGAACAGCGGCTGCACGCGGGAG  >  NZ_CP009273/3193815‑3193986

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑