Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,202,275	T→G	V298G (GTG→GGG)	ttdT →	L‑tartrate/succinate antiporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,202,275	0	T	G	100.0%	167.7 / NA	45	V298G (GTG→GGG)	ttdT	L‑tartrate/succinate antiporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (18/27);  total (18/27)

CGCGCTGGGCAGAGACGGAACTGCAGGCAATGGGCCCGCTCTGTTCGCGTGAAAAACGGATGCTGGGGCTGATGGTAGGCGCGCTGGTGCTGTGGATTTTCGGCGGTGATTATATCGATGCCGCGATGGTCGGTTACAGCGTGGTGGCACTGATGCTGCTTCTGCGCATTATC  >  NZ_CP009273/3202188‑3202360                                                                                        |                                                                                      cgcgCTGGGCAGAGACGGAACTGCAGGCAATGGGCCCGCTCTGTTCGCGTGAAAAACGGATGCTGGGGCTGATGGTAGGCGCGCTGGGGCt                                                                                    <  2:146588/91‑1 (MQ=255)  gcgcTGGGCAGAGACGGAACTGCAGGCAATGGGCCCGCTCTGTTCGCGTGAAAAACGGATGCTGGGGCTGATGGTAGGCGCGCTGGGGCtg                                                                                   >  1:485005/1‑91 (MQ=255)   cgcTGGGCAGAGACGGAACTGCAGGCAATGGGCCCGCTCTGTTCGCGTGAAAAACGGATGCTGGGGCTGATGGTAGGCGCGCTGGGGCtgt                                                                                  <  2:848148/91‑1 (MQ=255)      tGGGCAGAGACGGAACTGCAGGCAATGGGCCCGCTCTGTTCGCGTGAAAAACGGATGCTGGGGCTGATGGTAGGCGCGCTGGGGCTGTGGa                                                                               >  1:196692/1‑91 (MQ=255)            gagaCGGAACTGCAGGCAATGGGCCCGCTCTGTTCGCGTGAAAAACGGATGCTGGGGCTGATGGTAGGCGCGCTGGGGCTGTGGATTTTcg                                                                         >  1:148465/1‑91 (MQ=255)            gagaCGGAACTGCAGGCAATGGGCCCGCTCTGTTCGCGTGAAAAACGGATGCTGGGGCTGATGGTAGGCGCGCTGGGGCTGTGGATTTTcg                                                                         >  2:75124/1‑91 (MQ=255)               aCGGAACTGCAGGCAATGGGCCCGCTCTGTTCGCGTGAAAAACGGATGCTGGGGCTGATGGTAGGCGCGCTGGGGCTGTGGATTTTcggcg                                                                      <  1:231152/91‑1 (MQ=255)                cGGAACTGCAGGCAATGGGCCCGCTCTGTTCGCGTGAAAAACGGATGCTGGGGCTGATGGTAGGCGCGCTGGGGCTGTGGATTTTcggggg                                                                     <  2:695818/91‑1 (MQ=255)                cGGAACTGCAGGCAATGGGCCCGCTCTGTTCGCGTGAAAAACGGATGCTGGGGCTGATGGTAGGCGCGCTGGGGCTGTGGATTTTcggcgg                                                                     <  2:23389/91‑1 (MQ=255)                cGGAACTGCAGGCAATGGGCCCGCTCTGTTCGCGTGAAAAACGGATGCTGGGGCTGATGGTAGGCGCGCTGGGGCTGTGGATTTTcggcgg                                                                     <  2:745608/91‑1 (MQ=255)                cGGAACTGCAGGCAATGGGCCCGCTCTGTTCGCGTGAAAAACGGATGCTGGGGCTGATGGTAGGCGCGCTGGGGCTGTGGATTTTcggcgg                                                                     <  1:418513/91‑1 (MQ=255)                 ggAACTGCAGGCAATGGGCCCGCTCTGTTCGCGTGAAAAACGGATGCTGGGGCTGATGGTAGGCGCGCTGGGGCTGTGGATTTTCGGCGGt                                                                    >  1:824584/1‑91 (MQ=255)                 ggAACTGCAGGCAATGGGCCCGCTCTGTTCGCGTGAAAAACGGATGCTGGGGCTGATGGTAGGCGCGCTGGGGCTGTGGATTTTCGGCGGt                                                                    <  2:148465/91‑1 (MQ=255)                 ggAACTGCAGGCAATGGGCCCGCTCTGTTCGCGTGAAAAACGGATGCTGGGGCTGATGGTAGGCGCGCTGGGGCTGTGGATTTTCGGCGGt                                                                    >  1:363693/1‑91 (MQ=255)                 ggAACTGCAGGCAATGGGCCCGCTCTGTTCGCGTGAAAAACGGATGCTGGGGCTGATGGTAGGCGCGCTGGGGCTGTGGATTTTCGGCGGt                                                                    <  1:166678/91‑1 (MQ=255)                 ggAACTGCAGGCAATGGGCCCGCTCTGTTCGCGTGAAAAACGGATGCTGGGGCTGATGGTAGGCGCGCTGGGGCTGTGGATTTTCGGCGGt                                                                    <  2:756272/91‑1 (MQ=255)                 ggAACTGCAGGCAATGGGCCCGCTCTGTTCGCGTGAAAAACGGATGCTGGGGCTGATGGTAGGCGCGCTGGGGCTGTGGATTTTCGGCGGt                                                                    >  2:843396/1‑91 (MQ=255)                     cTGCAGGCAATGGGCCCGCTCTGTTCGCGTGAAAAACGGATGCTGGGGCTGATGGTAGGCGCGCTGGGGCTGTGGATTTTCGGCGGTGAtt                                                                <  2:746719/91‑1 (MQ=255)                                   cccGCTCTGTTCGCGTGAAAAACGGATGCTGGGGCTGATGGTAGGCGCGCTGGGGCTGTGGATTTTCGGCGGTGATTATATCGATGCcgcg                                                  >  1:266000/1‑91 (MQ=255)                                    ccGCTCTGTTCGCGTGAAAAACGGATGCTGGGGCTGATGGTAGGCGCGCTGGGGCTGTGGATTTTCGGCGGTGATTATATCGATGCCGCGa                                                 >  2:320822/1‑91 (MQ=255)                                     cGCTCTGTTCGCGTGAAAAACGGATGCTGGGGCTGATGGTAGGCGCGCTGGGGCTGTGGATTTTCGGCGGTGATTATATCGATGCCGCGAt                                                <  2:626991/91‑1 (MQ=255)                                     cGCTCTGTTCGCGTGAAAAACGGATGCTGGGGCTGATGGTAGGCGCGCTGGGGCTGTGGATTTTCGGCGGTGATTATATCGATGCCGCGAt                                                <  2:184426/91‑1 (MQ=255)                                      gCTCTGTTCGCGTGAAAAACGGATGCTGGGGCTGATGGTAGGCGCGCTGGGGCTGTGGATTTTCGGCGGTGATTATATCGATGCCGCGATg                                               >  1:11736/1‑91 (MQ=255)                                      gCTCTGTTCGCGTGAAAAACGGATGCTGGGGCTGATGGTAGGCGCGCTGGGGCTGTGGATTTTCGGCGGTGATTATATCGATGCCGCGATg                                               >  1:164569/1‑91 (MQ=255)                                       ctctGTTCGCGTGAAAAACGGATGCTGGGGCTGATGGTAGGCGCGCTGGGGCTGTGGATTTTCGGCGGTGATTATATCGATGCCGCAATgg                                              <  2:478577/91‑1 (MQ=255)                                         ctGTTCGCGTGAAAAACGGATGCTGGGGCTGATGGTAGGCGCGCTGGGGCTGTGGATTTTCGGCGGTGATTATATCGATGCCGCGATGGTc                                            <  1:262269/91‑1 (MQ=255)                                         ctGTTCGCGTGAAAAACGGATGCTGGGGCTGATGGTAGGCGCGCTGGGGCTGTGGATTTTCGGCGGTGATTATATCGATGCCGCGATGGTc                                            <  1:422182/91‑1 (MQ=255)                                         ctGTTCGCGTGAAAAACGGATGCTGGGGCTGATGGTAGGCGCGCTGGGGCTGTGGATTTTCAGCGGTGATTATATCGATGCCGCGATGGTc                                            <  1:210339/91‑1 (MQ=255)                                               gcgTGAAAAACGGATGCTGGGGCTGATGGTAGGCGCGCTGGGGCTGTGGATTTTCGGCGGTGATTATATCGATGCCGCGATGGTCGGTTAc                                      <  2:307648/91‑1 (MQ=255)                                                 gTGAAAAACGGATGCTGGGGCTGATGGTAGGCGCGCTGGGGCTGTGGATTTTCGGCGGTGATTATATCGATGCCGCGATGGTCGGTTACAg                                    >  2:59046/1‑91 (MQ=255)                                                 atGAAAAACGGATGCTGGGGCTGATGGTAGGCGCGCTGGGGCTGTGGATTTTCGGCGGTGATTATATCGATGCCGCGATGGTCGGTTACAg                                    >  1:79377/2‑91 (MQ=255)                                                  tGAAAAACGGATGCTGGGGCTGATGGTAGGCGCGCTGGGGCTGTGGATTTTCGGCGGTGATTATATCGATGCCGCGATGGTCGGTTACAGc                                   <  2:549062/91‑1 (MQ=255)                                                    aaaaaCGGATGCTGGGGCTGATGGTAGGCGCGCTGGGGCTGTGGATTTTCGGCGGTGATTATATCGATGCCGCGATGGTCGGTTACAGCgt                                 <  1:474249/91‑1 (MQ=255)                                                     aaaaCGGATGCTGGGGCTGATGGTAGGCGCGCTGGGGCTGTGGATTTTCGGCGGTGATTATATCGATGCCGCGATGGTCGGTTACAGCgtg                                >  1:581621/1‑91 (MQ=255)                                                       aaCGGATGCTGGGGCTGATGGTAGGCGCGCTGGGGCTGTGGATTTTCGGCGGTGATTATATCGATGCCGCGATGGTCGGTTACAGCgtggt                              >  2:268637/1‑91 (MQ=255)                                                           gATGCTGGGGCTGATGGTAGGCGCGCTGGGGCTGTGGATTTTCGGCGGTGATTATATCGATGCCGCGATGGTc                                            >  1:783266/1‑73 (MQ=255)                                                           gATGCTGGGGCTGATGGTAGGCGCGCTGGGGCTGTGGATTTTCGGCGGTGATTATATCGATGCCGCGATGGTc                                            <  2:783266/73‑1 (MQ=255)                                                              gCTGGGGCTGATGGTAGGCGCGCTGGGGCTGTGGATTTTCGGCGGTGATTATATCGATGCCGCGATGGTCGGTTACAGCGTGGTGGCACTg                       <  1:760726/91‑1 (MQ=255)                                                                           gTAGGCGCGCTGGGGCTGTGGATTTTCGGCGGTGATTATATCGATGCCGCGATGGTCGGTTACAGCGTGGTGGCACTGATGCTGCTTCTgc          <  1:560020/91‑1 (MQ=255)                                                                           gTAGGCGCGCTGGGGCTGTGGATTTTCGGCGGTGATTATATCGATGCCGCGATGGTCGGTTACAGCGTGGTGGCACTGATGCTGCTTCTgc          <  1:594409/91‑1 (MQ=255)                                                                           gTAGGCGCGCTGGGGCTGTGGATTTTCGGCGGTGATTATATCGATGCCGCGATGGTCGGTTACAGCGTGGTGGCACTGATGCTGCTTCTgc          <  1:153566/91‑1 (MQ=255)                                                                             aGGCGCGCTGGGGCTGTGGATTTTCGGCGGTGATTATATCGATGCCGCGATGGTCGGTTACAGCGTGGTGGCACTGATGCTGCTTCTgcgc        <  1:111504/91‑1 (MQ=255)                                                                                 gcgcTGGGGCTGTGGATTTTCGGCGGTGATTATATCGATGCCGCGATGGTCGGTTACAGCGTGGTGGCACTGATGCTGCTTCTGCGCATTa    >  1:184484/1‑91 (MQ=255)                                                                                   gcTGGGGCTGTGGATTTTCGGCGGTGATTATATCGATGCCGCGATGGTCGGTTACAGCGTGGTGGCACTGATGCTGCTTCTGCGCATTATc  >  1:312282/1‑91 (MQ=255)                                                                                   gcTGGGGCTGTGGATTTTCGGCGGTGATTATATCGATGCCGCGATGGTCGGTTACAGCGTGGTGGCACTGATGCTGCTTCTGCGCATTATc  <  2:266000/91‑1 (MQ=255)                                                                                        |                                                                                      CGCGCTGGGCAGAGACGGAACTGCAGGCAATGGGCCCGCTCTGTTCGCGTGAAAAACGGATGCTGGGGCTGATGGTAGGCGCGCTGGTGCTGTGGATTTTCGGCGGTGATTATATCGATGCCGCGATGGTCGGTTACAGCGTGGTGGCACTGATGCTGCTTCTGCGCATTATC  >  NZ_CP009273/3202188‑3202360

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑