Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,210,830	A→C	intergenic (+69/+85)	nfeR → / ← aer	DNA‑binding transcriptional regulator NfeR/aerotaxis sensor receptor Aer

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,210,830	0	A	C	100.0%	71.6 / NA	20	intergenic (+69/+85)	nfeR/aer	DNA‑binding transcriptional regulator NfeR/aerotaxis sensor receptor Aer
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (8/12);  total (8/12)

ATATTACGCAACTGGATTAATCGCCGCATCCGCCAGTGGCGCGGTGCAATTGCCGGATGCGACGCTTGACGCGCCTTATCCGGCCTACACCCGCTACACACCCCGCAGGCCTGATAAGATGCGCCAGCATCGCATCAGGCATTGTGCTCCAACCGCCGGATCCGGCATACCGATT  >  NZ_CP009273/3210742‑3210916                                                                                         |                                                                                       atatTACGCAACTGGATTAATCGCCGCATCCGCCAGTGGCGCGGTGCAATTGCCGGATGCGACGCTTGACGCGCCTTATCCGGCCTAcccc                                                                                      <  2:522448/91‑1 (MQ=255) atatTACGCAACTGGATTAATCGCCGCATCCGCCAGTGGCGCGGTGCAATTGCCGGATGCGACGCTTGACGCGCCTTATCCGGCCTAcccc                                                                                      <  1:689776/91‑1 (MQ=255)         cAACTGGATTAATCGCCGCATCCGCCAGTGGCGCGGTGCAATTGCCGGATGCGACGCTTGACGCGCCTTATCCGGCCTACCCCCGCTacac                                                                              <  2:495827/91‑1 (MQ=255)          aaCTGGATTAATCGCCGCATCCGCCAGTGGCGCGGTGCAATTGCCGGATGCGACGCTTGACGCGCCTTATCCGGCCTACCCCCGCTacaca                                                                             >  2:817231/1‑91 (MQ=255)          aaCTGGATTAATCGCCGCATCCGCCAGTGGCGCGGTGCAATTGCCGGATGCGACGCTTGACGCGCCTTATCCGGCCTACCCCCGCTacaca                                                                             <  2:690277/91‑1 (MQ=255)           aCTGGATTAATCGCCGCATCCGCCAGTGGCGCGGTGCAATTGCCGGATGCGACGCTTGACGCGCCTTATCCGGCCTACCCCCGCTacacac                                                                            <  1:126429/91‑1 (MQ=255)              ggATTAATCGCCGCATCCGCCAGTGGCGCGGTGCAATTGCCGGATGCGACGCTTGACGCGCCTTATCCGGCCTACCCCCGCTACACAcccc                                                                         >  2:92582/1‑91 (MQ=255)                  tAATCGCCGCATCCGCCAGTGGCGCGGTGCAATTGCCGGATGCGACGCTTGACGCGCCTTATCCGGCCTACCCCCGCTACACACCCCGCAg                                                                     <  1:43703/91‑1 (MQ=255)                   aaTCGCCGCATCCGCCAGTGGCGCGGTGCAATTGCCGGATGCGACGCTTGACGCGCCTTATCCGGCCTACCCCCGCTACACACCCCGCAgg                                                                    >  1:695720/1‑91 (MQ=255)                     tCGCCGCATCCGCCAGTGGCGCGGTGCAATTGCCGGATGCGACGCTTGACGCGCCTTATCCGGCCTACCCCCGCTACACACCCCGCAGGcc                                                                  >  2:386419/1‑91 (MQ=255)                          gcATCCGCCAGTGGCGCGGTGCAATTGCCGGATGCGACGCTTGACGCGCCTTATCCGGCCTACCCCCGCTACACACCCCGCAGGCCTGATa                                                             >  1:136761/1‑91 (MQ=255)                                  cAGTGGCGCGGGGCAATTGCCGGATCCGACGCTTGACGCGCCTTATCCGGCCAACCCCCGCTACACACCCCGCAGCCCTGATAAGATgcgc                                                     >  2:844598/1‑91 (MQ=25)                                       gcgcGGTGCAATTGCCGGATGCGACGCTTGACGCGCCTTATCCGGCCTACCCCCGCTACACACCCCGCAGGCCTGATAAGATGCGCCAgca                                                >  1:104570/1‑91 (MQ=255)                                        cgcgGTGCAATTGCCGGATGCGACGCTTGACGCGCCTTATCCGGCCTACCCCCGCTACACACCCCGCAGGCCTGATAAGATGCGCCAgcat                                               <  1:224448/91‑1 (MQ=255)                                                     ccGGATGCGACGCTTGACGCGCCTTATCCGGCCTACCCCCGCTACACACCCCGCAGGCCTGATAAGATGCGCCAGCATCGCATCAGGCAtt                                  <  1:652981/91‑1 (MQ=255)                                                     ccGGATGCGACGCTTGACGCGCCTTATCCGGCCTACCCCCGCTACACACCCCGCAGGCCTGATAAGATGCGCCAGCATCGCATCAGGCAtt                                  <  1:516609/91‑1 (MQ=255)                                                             gACGCTTGACGCGCCTTATCCGGCCTACCCCCGCTACACACCCCGCAGGCCTGATAAGATGCGCCAGCATCGCATCAGGCATTGTGCTCCa                          >  1:670830/1‑91 (MQ=255)                                                                      cgcgCCTTATCCGGCCTACCCCCGCTACACACCCCGCAGGCCTGATAAGATGCGCCAGCATCGCATCAGGCATTGTGCTCCAACCGCCGGa                 <  2:104570/91‑1 (MQ=255)                                                                                ccGGCCTACCCCCGCTACACACCCCGCAGGCCTGATAAGATGCGCCAGCATCGCATCAGGCATTGTGCTCCAACCGCCGGATCCGGCATAc       <  1:707386/91‑1 (MQ=255)                                                                                ccGGCCTACCCCCGCTACACACCCCGCAGGCCTGATAAGATGCGCCAGCATCGCATCAGGCATTGTGCTCCAACCGCCGGATCCGGCATAc       <  1:43046/91‑1 (MQ=255)                                                                                    ccTACCCCCGCTACACACCCCGCAGGCCTGATAAGATGCGCCAGCATCGCATCAGGCATTGTGCTCCAACCGCCGGATCCGGCATACCGAt   >  1:832375/1‑91 (MQ=255)                                                                                     cTACCCCCGCTACACACCCCGCAGGCCTGATAAGATGCGCCAGCATCGCATCAGGCATTGTGCTCCAACCGCCGGATCCGGCATACCGAtt  <  1:660437/91‑1 (MQ=255)                                                                                     cTACCCCCGCTACACACCCCGCAGGCCTGATAAGATGCGCCAGCATCGCATCAGGCATTGTGCTCCAACCGCCGGATCCGGCATACCGAtt  <  1:817231/91‑1 (MQ=255)                                                                                         |                                                                                       ATATTACGCAACTGGATTAATCGCCGCATCCGCCAGTGGCGCGGTGCAATTGCCGGATGCGACGCTTGACGCGCCTTATCCGGCCTACACCCGCTACACACCCCGCAGGCCTGATAAGATGCGCCAGCATCGCATCAGGCATTGTGCTCCAACCGCCGGATCCGGCATACCGATT  >  NZ_CP009273/3210742‑3210916

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑