Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	453,695	G→A	A272T (GCG→ACG)	clpX →	ATP‑dependent protease ATP‑binding subunit ClpX

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	453,695	0	G	A	100.0%	138.9 / NA	36	A272T (GCG→ACG)	clpX	ATP‑dependent protease ATP‑binding subunit ClpX
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (19/17);  total (19/17)

CCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCTGGATAAAGTGATTTCCCACCGTGTAGAAACCGGCTCCGGCATTGGTTTTGGCGCGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCT  >  NZ_CP009273/453610‑453780                                                                                      |                                                                                      ccTGTTTATTTGTGGCGGTGCGTTTGCCGGTCTGGATAAAGTGATTTCCCACCGTGTAGAAACCGGCTCCGGCATTGGTTTTGGCacgacg                                                                                  >  1:712539/1‑91 (MQ=255) ccTGTTTATTTGTGGCGGTGCGTTTGCCGGTCTGGATAAAGTGATTTCCCACCGTGTAGAAACCGGCTCCGGCATTGGTTTTGGCacgacg                                                                                  >  1:648248/1‑91 (MQ=255) ccTGTTTATTTGTGGCGGTGCGTTTGCCGGTCTGGATAAAGTGATTTCCCACCGTGTAGAAACCGGCTCCGGCATTGGTTTTGGCacgacg                                                                                  >  1:340373/1‑91 (MQ=255)  cTGTTTATTTGTGGCGGTGCGTTTGCCGGTCTGGATAAAGTGATTTCCCACCGTGTAGAAACCGGCTCCGGCATTGGTTTTGGCACGACgg                                                                                 >  2:855004/1‑91 (MQ=255)  cTGTTTATTTGTGGCGGTGCGTTTGCCGGTCTGGATAAAGTGATTTCCCACCGTGTAGAAACCGGCTCCGGCATTGGTTTTGGCACGACgg                                                                                 >  2:766055/1‑91 (MQ=255)  cTGTTTATTTGTGGCGGTGCGTTTGCCGGTCTGGATAAAGTGATTTCCCACCGTGTAGAAACCGGCTCCGGCATTGGTTTTGGCACGACgg                                                                                 >  2:459242/1‑91 (MQ=255)    gTTTATTTGTGGCGGTGCGTTTGCCGGTCTGGATAAAGTGATTTCCCACCGTGTAGAAACCGGCTCCGGCATTGGTTTTGGCACGACGGTa                                                                               >  1:53157/1‑91 (MQ=255)         tttGTGGCGGTGCGTTTGCCGGTCTGGATAAAGTGATTTCCCACCGTGTAGAAACCGGCTCCGGCATTGGTTTTGGCACGACGGTAAAAGc                                                                          <  2:662826/91‑1 (MQ=255)         tttGTGGCGGTGCGTTTGCCGGTCTGGATAAAGTGATTTCCCACCGTGTAGAAACCGGCTCCGGCATTGGTTTTGGCACGACGGTAAAAGc                                                                          <  2:648248/91‑1 (MQ=255)           tgtgGCGGTGCGTTTGCCGGTCTGGATAAAGTGATTTCCCACCGTGTAGAAACCGGCTCCGGCATTGGTTTTGGCACGACGGTAAAAGCGa                                                                        <  1:62350/91‑1 (MQ=255)           tgtgGCGGTGCGTTGGCCGGTCTGGATAAAGTGATTTCCCACCGTGTAGAAACCGGCTCCGGCATTGGTTTTGGCACGACGGTAAAAGCGa                                                                        <  2:35132/91‑1 (MQ=255)             tgGCGGTGCGTTTGCCGGTCTGGATAAAGTGATTTCCCACCGTGTAGAAACCGGCTCCGGCATTGGTTTTGGCACGACGGTAAAAGCGAAg                                                                      <  1:459242/91‑1 (MQ=255)              ggCGGTGCGTTTGCCGGTCTGGATAAAGTGATTTCCCACCGTGTAGAAACCGGCTCCGGCATTGGTTTTGGCACGACGGTAAAAGCGAAGt                                                                     >  1:195275/1‑91 (MQ=255)              ggCGGTGCGTTTGCCGGTCTGGATAAAGTGATTTCCCACCGTGTAGAAACCGGCTCCGGCATTGGTTTTGGCACGACGGTAAAAGCGAAGt                                                                     >  1:341528/1‑91 (MQ=255)                        ttGCCGGTCTGGATAAAGTGATTTCCCACCGTGTAGAAACCGGCTCCGGCATTGGTTTTGGCACGACGGTAAAAGCGAAGTCCGACAaagc                                                           <  1:244626/91‑1 (MQ=255)                        ttGCCGGTCTGGATAAAGTGATTTCCCACCGTGTAGAAACCGGCTCCGGCATTGGTTTTGGCACGACGGTAAAAGCGAAGTCCGACAaagc                                                           <  1:658821/91‑1 (MQ=255)                         tGCCGGTCTGGATAAAGTGATTTCCCACCGTGTAGAAACCGGCTCCGGCATTGGTTTTGGCACGACGGTAAAAGCGAAGTCCGACAaagca                                                          >  1:439957/1‑91 (MQ=255)                              gTCTGGATAAAGTGATTTCCCACCGTGTAGAAACCGGCTCCGGCATTGGTTTTGGCACGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGa                                                     >  1:173497/1‑91 (MQ=255)                              gTCTGGATAAAGTGATTTCCCACCGTGTAGAAACCGGCTCCGGCATTGGTTTTGGCACGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGa                                                     >  1:480862/1‑91 (MQ=255)                                            aTTTCCCACCGTGTAGAAACCGGCTCCGGCATTGGTTTTGGCACGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTgg                                       >  1:420177/1‑91 (MQ=255)                                                 ccACCGTGTAGAAACCGGCTCCGGCATTGGTTTTGGCACGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAg                                  >  1:332787/1‑91 (MQ=255)                                                    ccGTGTAGAAACCGGCTCCGGCATTGGTTTTGGCACGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGtt                               <  1:187686/91‑1 (MQ=255)                                                      gtgtAGAAACCGGCTCCGGCATTGGTTTTGGCACGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGa                             >  1:4433/1‑91 (MQ=255)                                                            aaaCCGGCTCCGGCATTGGTTTTGGCACGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGa                       <  1:766055/91‑1 (MQ=255)                                                             aaCCGGCTCCGGCATTGGTTTTGGCACGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGaa                      <  1:469996/91‑1 (MQ=255)                                                              aCCGGCTCCGGCATTGGTTTTGGCACGACGGTAAAAGCAAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAg                     >  1:295576/1‑91 (MQ=255)                                                                cGGCTCCGGCATTGGTTTTGGCACGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGAt                   >  1:775043/1‑91 (MQ=255)                                                                cGGCTCCGGCATTGGTTTTGGCACGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGAt                   >  2:597695/1‑91 (MQ=255)                                                                 ggCTCCGGCATTGGTTTTGGCACGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATc                  >  2:375675/1‑91 (MQ=255)                                                                  gCTCCGGCATTGGTTTTGGCACGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCt                 >  1:100381/1‑91 (MQ=255)                                                                  gCTCCGGCATTGGTTTTGGCACGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCt                 >  1:297072/1‑91 (MQ=255)                                                                      cGGCATTGGTTTTGGCACGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATc             <  2:340373/91‑1 (MQ=255)                                                                      cGGCATTGGTTTTGGCACGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATc             <  2:313378/91‑1 (MQ=255)                                                                      cGGCATTGGTTTTGGCACGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATc             <  2:100381/91‑1 (MQ=255)                                                                      cGGCATTGGTTTTGGCACGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATc             <  1:496583/91‑1 (MQ=255)                                                                       ggCATTGGTTTTGGCACGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCa            <  2:101557/91‑1 (MQ=255)                                                                           ttGGTTTTGGCACGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGtt        <  1:597695/91‑1 (MQ=255)                                                                            tGGTTTTGGCACGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGttt       >  1:789888/1‑91 (MQ=255)                                                                                 ttGGCACGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCt  <  2:341528/91‑1 (MQ=255)                                                                                      |                                                                                      CCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCTGGATAAAGTGATTTCCCACCGTGTAGAAACCGGCTCCGGCATTGGTTTTGGCGCGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCT  >  NZ_CP009273/453610‑453780

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑