Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,232,610	T→C	I224I (ATT→ATC)	alx →	TerC family membrane protein Alx

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,232,610	0	T	C	100.0%	111.0 / NA	30	I224I (ATT→ATC)	alx	TerC family membrane protein Alx
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (10/20);  total (10/20)

ATGGATTGCTGTACGCCACACCGCTGATGCTGGTACTGATTCTGGTGGAATTGAGCGACGTGATTTTCGCCGTGGATAGCATTCCGGCTATCTTCGCCGTGACCACTGACCCGTTCATTGTGCTGACCTCAAACCTGTTTGCGATCCTCGGCCTGCGTGCGATGTAT  >  NZ_CP009273/3232528‑3232694                                                                                   |                                                                                     aTGGATTGCTGTACGCCACACCGCTGATGCTGGTACTGATTCTGGTGGAATTGAGCGACGTGATTTTCGCCGTGGATAGCATCCCGGCTAt                                                                              >  1:707194/1‑91 (MQ=255)      ttGCTGTACGCCACACCGCTGATGCTGGTACTGATTCTGGTGGAATTGAGCGACGTGATTTTCGCCGTGGATAGCATCCCGGCTATCTTCg                                                                         >  2:668729/1‑91 (MQ=255)                ccACACCGCTGATGCTGGTACTGATTCTGGTGGAATTGAGCGACGTGATTTTCGCCGTGGATAGCATCCCGGCTATCTTCGCCGTGACCAc                                                               <  2:698888/91‑1 (MQ=255)                  acacCGCTGATGCTGGTACTGATTCTGGTGGAATTGAGCGACGTGATTTTCGCCGTGGATAGCATCCCGGCTATCTTCGCCGTGACCACTg                                                             >  1:264712/1‑91 (MQ=255)                  acacCGCTGATGCTGGTACTGATTCTGGTGGAATTGAGCGACGTGATTTTCGCCGTGGATAGCATCCCGGCTATCTTCGCCGTGACCACTg                                                             <  1:600617/91‑1 (MQ=255)                  acacCGCTGATGCTGGTACTGATTCTGGTGGAATTGAGCGACGTGATTTTCGCCGTGGATAGCATCCCGGCTATCTTCGCCGTGACCACTg                                                             <  2:576943/91‑1 (MQ=255)                  acacCGCTGATGCTGGTACTGATTCTGGTGGAATTGAGCGACGTAATTTTCGCCGTGGATAGCATCCCGGCTATCTTCGCCGTGACCACTg                                                             <  1:613915/91‑1 (MQ=255)                    acCGCTGATGCTGGTACTGATTCTGGTGGAATTGAGCGACGTGATTTTCGCCGTGGATAGCATCCCGGCTATCTTCGCCGTGACCACTGAc                                                           <  2:707194/91‑1 (MQ=255)                     ccGCTGATGCTGGTACTGATTCTGGTGGAATTGAGCGACGTGATTTTCGCCGTGGATAGCATCCCGGCTATCTTCGCCGTGACCACTGAcc                                                          <  2:177130/91‑1 (MQ=255)                       gCTGATGCTGGTACTGATTCTGGTGGAATTGAGCGACGTGATTTTCGCCGTGGATAGCATCCCGGCTATCTTCGCCGTGACCACTGACCCg                                                        >  1:643090/1‑91 (MQ=255)                                  tACTGATTCTGGTGGAATTGAGCGACGTGATTTTCGCCGTGGATAGCATCCCGGCTATCTTCGCCGTGACCACTGACCCGTTCATTGTGCt                                             <  1:227381/91‑1 (MQ=255)                                   aCTGATTCTGGTGGAATTGAGCGACGTGATTTTCGCCGTGGATAGCATCCCGGCTATCTTCGCCGTGACCACTGACCCGTTCATTGTGCTg                                            <  2:672736/91‑1 (MQ=255)                                   aCTGATTCTGGTGGAATTGAGCGACGTGATTTTCGCCGTGGATAGCATCCCGGCTATCTTCGCCGTGACCACTGACCCGTTCATTGTGCTg                                            <  1:488110/91‑1 (MQ=255)                                   aCTGATTCTGGTGGAATTGAGCGACGTGATTTTCGCCGTGGATAGCATCCCGGCTATCTTCGCCGTGACCACTGACCCGTTCATTGTGCTg                                            <  1:847242/91‑1 (MQ=255)                                    cTGATTCTGGTGGAATTGAGCGACGTGATTTTCGCCGTGGATAGCATCCCGGCTATCTTCGCCGTGACCACTGACCCGTTCATTGTGCTGa                                           >  2:613710/1‑91 (MQ=255)                                      gATTCTGGTGGAATTGAGCGACGTGATTTTCGCCGTGGATAGCATCCCGGCTATCTTCGCCGTGACCACTGACCCGTTCATTGTGCTGAcc                                         >  2:633256/1‑91 (MQ=255)                                      gATTCTGGTGGAATTGAGCGACGTGATTTTCGCCGTGGATAGCATCCCGGCTATCTTCGCCGTGACCACTGACCCGTTCATTGTGCTGAcc                                         >  1:254732/1‑91 (MQ=255)                                      gATTCTGGTGGAATTGAGCGACGTGATTTTCGCCGTGGATAGCATCCCGGCTATCTTCGCCGTGACCACTGACCCGTTCATTGTGCTGAcc                                         >  2:329337/1‑91 (MQ=255)                                      gATTCTGGTGGAATTGAGCGACGTGATTTTCGCCGTGGATAGCATCCCGGCTATCTTCGCCGTGACCACTGACCCGTTCATTGTGCTGAcc                                         >  2:195344/1‑91 (MQ=255)                                                 aaTTGAGCGACGTGATTTTCGCCGTGGATAGCATCCCGGCTATCTTCGCCGTGACCACTGACCCGTTCATTGTGCTg                                            >  2:664483/1‑77 (MQ=255)                                                 aaTTGAGCGACGTGATTTTCGCCGTGGATAGCATCCCGGCTATCTTCGCCGTGACCACTGACCCGTTCATTGTGCTg                                            <  1:664483/77‑1 (MQ=255)                                                     gAGCGACGTGATTTTCGCCGTGGATAGCATCCCGGCTATCTTCGCCGTGACCACTGACCCGTTCATTGTGCTGACCTCAAACCTGTTTGCg                          <  1:84908/91‑1 (MQ=255)                                                     gAGCGACGTGATTTTCGCCGTGGATAGCATCCCGGCTATCTTCGCCGTGACCACTGACCCGTTCATTGTGCTGACCTCAAACCTGTTTGCg                          <  1:305976/91‑1 (MQ=255)                                                               aTTTTCGCCGTGGATAGCATCCCGGCTATCTTCGCCGTGACCACTGACCCGTTCATTGTGCTGACCTCAAACCTGTTTGCGATCCTCGGcc                <  1:329337/91‑1 (MQ=255)                                                                      ccGTGGATAGCATCCCGGCTATCTTCGCCGTGACCACTGACCCGTTCATTGTGCTGACCTCAAACCTGTTTGCGATCCTCGGCCtgcgtgc         <  1:698368/91‑1 (MQ=255)                                                                       cGTGGATAGCATCCCGGCTATCTTCGCCGTGACCACTGACCCGTTCATTGTGCTGACCTCAAACCTGTTTGCGATCCTCGGCCtgcgtgcg        <  2:340509/91‑1 (MQ=255)                                                                       cGTGGATAGCATCCCGGCTATCTTCGCCGTGACCACTGACCCGTTCATTGTGCTGACCTCAAACCTGTTTGCGATCCTCGGCCtgcgtgcg        <  1:451253/91‑1 (MQ=255)                                                                        gTGGATAGCATCCCGGCTATCTTCGCCGTGACCACTGACCCGTTCATTGTGCTGACCTCAAACCTGTTTGCGATCCTCGGCCTGCGTGCGa       <  2:686312/91‑1 (MQ=255)                                                                         tGGATAGCATCCCGGCTATCTTCGCCGTGACCACTGACCCGTTCATTGTGCTGACCTCAAACCTGTTTGCGATCCTCGGCCTGCGTGCGAt      <  1:386581/91‑1 (MQ=255)                                                                             tAGCATCCCGGCTATCTTCGCCGTGACCACTGACCCGTTCATTGTGCTGACCTCAAACCTGTTTGCGATCCTCGGCCTGCGTCCGATGTAt  <  2:860666/91‑1 (MQ=255)                                                                                   |                                                                                     ATGGATTGCTGTACGCCACACCGCTGATGCTGGTACTGATTCTGGTGGAATTGAGCGACGTGATTTTCGCCGTGGATAGCATTCCGGCTATCTTCGCCGTGACCACTGACCCGTTCATTGTGCTGACCTCAAACCTGTTTGCGATCCTCGGCCTGCGTGCGATGTAT  >  NZ_CP009273/3232528‑3232694

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑