Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,326,882	A→G	intergenic (‑72/‑187)	rplU ← / → ispB	50S ribosomal protein L21/octaprenyl diphosphate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,326,882	0	A	G	100.0%	143.9 / NA	38	intergenic (‑72/‑187)	rplU/ispB	50S ribosomal protein L21/octaprenyl diphosphate synthase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (25/13);  total (25/13)

AAAACCGCGTACATATAAAACTCCGCTTCCGCGCACACCTTTTCAATGATTCAGAGTGCGCTATAAATATTCACAATAGGGCGCGAATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAGAAAAAAAACACAACTTGTACGGTAACGTTTATCT  >  NZ_CP009273/3326797‑3326967                                                                                      |                                                                                      aaaaCCGCGTACATATAAAACTCCGCTTCCGCGCACACCTTTTCAATGATTCAGAGTGCGCTATAAATATTCACAATAGGGCGCGGATAtt                                                                                  <  2:278350/91‑1 (MQ=255)    aCCGCGTACATATAAAACTCCGCTTCCGCGCACACCTTTTCAATGATTCAGAGTGCGCTATAAATATTCACAATAGGGCGCGGATATTACg                                                                               >  1:763852/1‑91 (MQ=255)     ccGCGTACATATAAAACTCCGCTTCCGCGCACACCTTTTCAATGATTCAGAGTGCGCTATAAATATTCACAATAGGGCGCGGATATTACGc                                                                              >  1:738744/1‑91 (MQ=255)     ccGCGTACATATAAAACTCCGCTTCCGCGCACACCTTTTCAATGATTCAGAGTGCGCTATAAATATTCACAATAGGGCGCGGATATTACGc                                                                              >  1:431428/1‑91 (MQ=255)       gcgTACATATAAAACTCCGCTTCCGCGCACACCTTTTCAATGATTCAGAGTGCGCTATAAATATTCACAATAGGGCGCGGATATTACGCaa                                                                            >  1:404732/1‑91 (MQ=255)              tataAAACTCCGCTTCCGCGCACACCTTTTCAATGATTCAGAGTGCGCTATAAATATTCACAATAGGGCGCGGATATTACGCAAAacgcac                                                                     <  1:132106/91‑1 (MQ=255)              tataAAACTCCGCTTCCGCGCACACCTTTTCAATGATTCAGAGTGCGCTATAAATATTCACAATAGGGCGCGGATATTACGCAAAacgcac                                                                     <  2:221343/91‑1 (MQ=255)              tataAAACTCCGCTTCCGCGCACACCTTTTCAATGATTCAGAGTGCGCTATAAATATTCACAATAGGGCGCGGATATTACGCAAAacgcac                                                                     <  1:500513/91‑1 (MQ=255)                taAAACTCCGCTTCCGCGCACTCCTTTTCAATGATGCAGAGTGCGCTATAAATAGTCACAATAGGGCGCAGATATTACGCAAAacgcacgc                                                                   <  2:868520/91‑1 (MQ=255)                      tCCGCTTCCGCGCACACCTTTTCAATGATTCAGAGTGCGCTATAAATATTCACAATAGGGCGCGGATATTACGCAAAACGCACGCCTTTGa                                                             >  2:649566/1‑91 (MQ=255)                      tCCGCTTCCGCGCACACCTTTTCAATGATTCAGAGTGCGCTATAAATATTCACAATAGGGCGCGGATATTACGCAAAACGCACGCCTTTGa                                                             >  1:242006/1‑91 (MQ=255)                       ccGCTTCCGCGCACACCTTTTCAATGATTCAGAGTGCGCTATAAATATTCACAATAGGGCGCGGATATTACGCAAAACGCACGCCTTTGAc                                                            >  1:17046/1‑91 (MQ=255)                        cGCTTCCGCGCACACCTTTTCAATGATTCAGAGTGCGCTATAAATATTCACAATAGGGCGCGGATATTACGCAAAACGCACGCCTTTGACa                                                           <  1:18821/91‑1 (MQ=255)                        cGCTTCCGCGCACACCTTTTCAATGATTCAGAGTGCGCTATAAATATTCACAATAGGGCGCGGATATTACGCAAAACGCACGCCTTTGACa                                                           >  1:785190/1‑91 (MQ=255)                               gcgcACACCTTTTCAATGATTCAGAGTGCGCTATAAATATTCACAATAGGGCGCGGATATTACGCAAAACGCACGCCTTTGACAAGTGCTa                                                    <  1:430186/91‑1 (MQ=255)                                 gcACACCTTTTCAATGATTCAGAGTGCGCTATAAATATTCACAATAGGGCGCGGATATTACGCAAAACGCACGCCTTTGACAAGTGCTACa                                                  <  1:88431/91‑1 (MQ=255)                                                gATTCAGAGTGCGCTATAAATATTCACAATAGGGCGCGGATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACgaaga                                   >  2:568026/1‑91 (MQ=255)                                                gATTCAGAGTGCGCTATAAATATTCACAATAGGGCGCGGATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACgaaga                                   >  1:458314/1‑91 (MQ=255)                                                          gcgcTATAAATATTCACAATAGGGCGCGGATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAGAAAAAAAacac                         >  1:375438/1‑91 (MQ=255)                                                          gcgcTATAAATATTCACAATAGGGCGCGGATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAGAAAAAAAacac                         >  1:170859/1‑91 (MQ=255)                                                              tataAATATTCACAATAGGGCGCGGATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAGAAAAAAAACACAACt                     <  1:22773/91‑1 (MQ=255)                                                                    tatTCACAATAGGGCGCGGATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAGAAAAAAAACACAACTTGTACg               >  1:34527/1‑91 (MQ=255)                                                                       tCACAATAGGGCGCGGATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAGAAAAAAAACACAACTTGTACGGTa            >  1:727243/1‑91 (MQ=255)                                                                       tCACAATAGGGCGCGGATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAGAAAAAAAACACAACTTGTACGGTa            <  2:422879/91‑1 (MQ=255)                                                                       tCACAATAGGGCGCGGATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAGAAAAAAAACACAACTTGTACGGTa            <  2:363559/91‑1 (MQ=255)                                                                       tCACAATAGGGCGCGGATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAGAAAAAAAACACAACTTGTACGGTa            >  1:480700/1‑91 (MQ=255)                                                                         acaATAGGGCGCGGATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAGAAAAAAAACACAACTTGTACGGTAAc          >  1:140139/1‑91 (MQ=255)                                                                         acaATAGGGCGCGGATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAGAAAAAAAACACAACTTGTACGGTAAc          >  1:160632/1‑91 (MQ=255)                                                                             tAGGGCGCGGATATTACGCAAAACGCACGCCTTTGACa                                                           >  1:742865/1‑38 (MQ=38)                                                                             tAGGGCGCGGATATTACGCAAAACGCACGCCTTTGACa                                                           <  2:742865/38‑1 (MQ=38)                                                                               gggCGCGGATATTACGCAAAACGCACGCCTTTGACAAGTGc                                                      >  1:521630/1‑41 (MQ=255)                                                                               gggCGCGGATATTACGCAAAACGCACGCCTTTGACAAGTGc                                                      <  2:521630/41‑1 (MQ=255)                                                                               gggCGCGGATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAGAAAAAAAACACAACt                     <  1:501555/74‑1 (MQ=255)                                                                               gggCGCGGATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAGAAAAAAAACACAACt                     >  2:501555/1‑74 (MQ=255)                                                                               gggCGCGGATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAGAAAAAAAACACAACTTGTACGGTAACGTTTAt    >  1:742535/1‑91 (MQ=255)                                                                                 gcgcGGATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAGAAAAAAAACACAACTTGTACGTTAACGTTTATCt  >  2:813788/1‑91 (MQ=255)                                                                                 gcgcGGATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAGAAAAAAAACACAACTTGTACGGTAACGTTTATCt  >  1:729058/1‑91 (MQ=255)                                                                                 gcgcGGATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAGAAAAAAAACACAACTTGTACGGTAACGTTTATCt  >  1:773727/1‑91 (MQ=255)                                                                                 gcgcGGATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAGAAAAAAAACACAACTTGTACGGTAACGTTTATCt  >  1:129006/1‑91 (MQ=255)                                                                                      |                                                                                      AAAACCGCGTACATATAAAACTCCGCTTCCGCGCACACCTTTTCAATGATTCAGAGTGCGCTATAAATATTCACAATAGGGCGCGAATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAGAAAAAAAACACAACTTGTACGGTAACGTTTATCT  >  NZ_CP009273/3326797‑3326967

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑