Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,437,182	A→G	I92T (ATC→ACC)	secY ←	preprotein translocase subunit SecY

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,437,182	0	A	G	100.0%	116.8 / NA	32	I92T (ATC→ACC)	secY	preprotein translocase subunit SecY
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (9/23);  total (9/23)

GCGGGTGTACTGGCTGATCTTACGACGACCAGACTCCCCTTCTTTCTTAATTTCTGCCAACGTTGGGTGAACCACCGTCAGCAGCTGGATAATGATCGACGCCGAAATATACGGCATGATCCCCAGAGCAAAGATAGAAGCACGGCTGAGAGCACCACCAGAGAACATGTTAAACA  >  NZ_CP009273/3437094‑3437269                                                                                         |                                                                                        gCGGGTGTACTGGCTGATCTTACGACGACCAGACTCCCCTTCTTTCTTAATTTCTGCCAACGTTGGGTGAACCACCGTCAGCAGCTGGGTa                                                                                       >  1:346668/1‑91 (MQ=255)      tgtACTGGCTGATCTTACGACGACCAGACTCCCCTTCTTTCTTAATTTCTGCCAACGTTGGGTGAACCACCGTCAGCAGCTGGGTAATGAt                                                                                  >  2:107954/1‑91 (MQ=255)       gtACTGGCTGATCTTACGACGACCAGACTCCCCTTCTTTCTTAATTGCTGCCAACGTTGGGTGAACCACCGTCAGCAGCGGGGTAATGATc                                                                                 <  2:143569/91‑1 (MQ=255)        tACTGGCTGATCTTACGACGACCAGACTCCCCTTCTTTCTTAATTTCTGCCAACGTTGGGTGAACCACCGTCAGCAGCTGGGTAATGATCg                                                                                >  2:507628/1‑91 (MQ=255)            ggCTGATCTTACGACGACCAGACTCCCCTTCTTTCTTAATTTCTGCCAACGTTGGGTGAACCACCGTCAGCAGCTGGGTAATGATCGACGc                                                                            <  2:108491/91‑1 (MQ=255)                 aTCTTACGACGACCAGACTCCCCTTCTTTCTTAATTTCTGCCAACGTTGGGTGAACCACCGTCAGCAGCTGGGTAATGATCGACGCCGaaa                                                                       >  1:647905/1‑91 (MQ=255)                      acgacgACCAGACTCCCCTTCTTTCTTAATTTCTGCCAACGTTGGGTGAACCACCGTCAGCAGCTGGGTAATGATCGACGCCGAAATATAc                                                                  <  1:15525/91‑1 (MQ=255)                        gacgacCAGACTCCCCTTCTTTCTTAATTTCTGCCAACGTTGGGTGAACCACCGTCAGCAGCTGGGTAATGATCGACGCCGAAATATACgg                                                                <  1:507628/91‑1 (MQ=255)                          cgacCAGACTCCCCTTCTTTCTTAATTTCTGCCAACGTTGGGTGAACCACCGTCAGCAGCTGGGTAATGATCGACGCCGAAATATACGGCa                                                              <  1:736497/91‑1 (MQ=255)                           gacCAGACTCCCCTTCTTTCTTAATTTCTGCCAACGTTGGGTGAACCACCGTCAGCAGCTGGGTAATGATCGACGCCGAAATATACGGCAt                                                             >  2:154738/1‑91 (MQ=255)                           gacCAGACTCCCCTTCTTTCTTAATTTCTGCCAACGTTGGGTGAACCACCGTCAGCAGCTGGGTAATGATCGACGCCGAAATATACGGCAt                                                             >  1:103962/1‑91 (MQ=255)                             ccAGACTCCCCTTCTTTCTTAATTTCTGCCAACGTTGGGTGAACCACCGTCAGCAGCTGGGTAATGATCGACGCCGAAATATACGGCATGa                                                           <  1:511480/91‑1 (MQ=255)                                   tCCCCTTCTTTCTTAATTTCTGCCAACGTTGGGTGAACCACCGTCAGCAGCTGGGTAATGATCGACGCCGAAATATACGGCATGATCCCCa                                                     <  1:444884/91‑1 (MQ=255)                                   tCCCCTTCTTTCTTAATTTCTGCCAACGTTGGGTGAACCACCGTCAGCAGCTGGGTAATGATCGACGCCGAAATATACGGCATGATCCCCa                                                     <  2:103962/91‑1 (MQ=255)                                        ttctttctTAATTTCTGCCAACGTTGGGTGAACCACCGTCAGCAGCTGGGTAATGATCGACGCCGAAATATACGGCATGATCCCCAGAGCa                                                <  2:123749/91‑1 (MQ=255)                                         tctttcttAATTTCTGCCAACGTTGGGTGAACCACCGTCAGCAGCTGGGTAATGATCGACGCCGAAATATACGGCATGATCCCCAGAGCaa                                               <  1:492511/91‑1 (MQ=255)                                         tctttcttAATTTCTGCCAACGTTGGGTGAACCACCGTCAGCAGCTGGGTAATGATCGACGCCGAAATATACGGCATGATCCCCAGAGCaa                                               <  1:638943/91‑1 (MQ=255)                                                tAATTTCTGCCAACGTTGGGTGAACCACCGTCAGCAGCTGGGTAATGATCGACGCCGAAATATACGGCATGATCCCCAGAGCAAAGATAGa                                        <  1:289210/91‑1 (MQ=255)                                                     tCTGCCAACGTTGGGTGAACCACCGTCAGCAGCTGGGTAATGATCGACGCCGAAATATACGGCATGATCCCCAGAGCAAAGATAGAAGCAc                                   <  1:327052/91‑1 (MQ=255)                                                          taaCGTTGGGTGAACCACCGTCAGCAGCTGGGTAATGATCGACGCCGAAATATACGGCATGATCCCCAGAGCAAAGATAGAAGCACGGCTg                              <  2:857427/90‑1 (MQ=255)                                                           aaCGTTGGGTGAACCACCGTCAGCAGCTGGGTAATGATCGACGCCGAAATATACGGCATGATCCCCAGAGCAAAGATAGAAGCACGGCTga                             <  2:70024/91‑1 (MQ=255)                                                                  ggTGAACCACCGTCAGCAGCTGGGTAATGATCGACGCCGAAATATACGGCATGATCCCCAGAGCAAAGATAGAAGCACGGCTGAGAGcacc                      <  1:356384/91‑1 (MQ=255)                                                                  gatGAACCACCGTCAGCAGCTGGGTAATGATCGACGCCGAAATATACGGCATGATCCCCAGAGCAAAGATAGAAGCACGGCTGAGAGcacc                      <  2:631234/89‑1 (MQ=255)                                                                      aaCCACCGTCAGCAGCTGGGTAATGATCGACGCCGAAATATACGGCATGATCCCCAGAGCAAAGATAGAAGCACGGCTGAGAGCAccacca                  >  1:6490/1‑91 (MQ=255)                                                                      aaCCACCGTCAGCAGCTGGGTAATGATCGACGCCGAAATATACGGCATGATCCCCAGAGCAAAGATAGAAGCACGGCTGAGAGCAccacca                  >  1:278276/1‑91 (MQ=255)                                                                       accaccGTCAGCAGCTGGGTAATGATCGACGCCGAAATATACGGCATGATCCCCAGAGCAAAGATAGAAGCACGGCTGAGAGCACCCCCag                 <  2:682785/91‑1 (MQ=255)                                                                           ccGTCAGCAGCTGGGTAATGATCGACGCCGAAATATACGGCATGATCCCCAGAGCAAAGATAGAAGCACGGCTGAGAGCACCACCAGAGaa             <  2:217089/91‑1 (MQ=255)                                                                           ccGTCAGCAGCTGGGTAATGATCGACGCCGAAATATACGGCATGATCCCCAGAGCAAAGATAGAAGCACGGCTGAGAGCACCACCAGAGaa             <  2:309739/91‑1 (MQ=255)                                                                           ccGTCAGCAGCTGGGTAATGATCGACGCCGAAATATACGGCATGATCCCCAGAGCAAAGATAGAAGCACGGCTGAGAGCACCACCAGAGaa             <  2:310939/91‑1 (MQ=255)                                                                            cGTCAGCAGCTGGGTAATGATCGACGCCGAAATATACGGCATGATCCCCAGAGCAAAGATAGAAGCACGGCTGAGAGCACCACCAGAGAAc            <  1:760002/91‑1 (MQ=255)                                                                                    gcTGGGTAATGATCGACGCCGAAATATACGGCATGATCCCCAGAGCAAAGATAGAAGCACGGCTGAGAGCACCACCAGAGAACATGTTaaa    >  1:842480/1‑91 (MQ=255)                                                                                      tGGGTAATGATCGACGCCGAAATATACGGCATGATCCCCAGAGCAAAGATAGAAGCACGGCTGAGAGCACCACCAGAGAACATGTTAAACa  <  1:420924/91‑1 (MQ=255)                                                                                         |                                                                                        GCGGGTGTACTGGCTGATCTTACGACGACCAGACTCCCCTTCTTTCTTAATTTCTGCCAACGTTGGGTGAACCACCGTCAGCAGCTGGATAATGATCGACGCCGAAATATACGGCATGATCCCCAGAGCAAAGATAGAAGCACGGCTGAGAGCACCACCAGAGAACATGTTAAACA  >  NZ_CP009273/3437094‑3437269

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑