Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,446,256	A→C	G10G (GGT→GGG)	rplC ←	50S ribosomal protein L3

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,446,256	0	A	C	100.0%	68.9 / NA	19	G10G (GGT→GGG)	rplC	50S ribosomal protein L3
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (10/9);  total (10/9)

GTCTTTAACCTGAGTAACGCGGTTTGCTTCAACTTCGATTACGGTTACTGGGATAGAAACGCCGTCTTCTGTGAAGATACGGGTCATACCCACTTTTTTACCGACTAAACCAATCATTGTTTCAACCTCTCAATCGCTCAATGACCTGATTAACCCAGGCTGA  >  NZ_CP009273/3446169‑3446331                                                                                        |                                                                            gTCTTTAACCTGAGTAACGCGGTTTGCTTCAACTTCGATTACGGTTACTGGGATAGAAACGCCGTCTTCTGTGAAGATACGGGTCATcccc                                                                          <  1:673592/91‑1 (MQ=255)     ttAACCTGAGTAACGCGGTTTGCTTCAACTTCGATTACGGTTACTGGGATAGAAACGCCGTCTTCTGTGAAGATACGGGTCATCCCCACtt                                                                      >  2:211474/1‑91 (MQ=255)           tGAGTAACGCGGTTTGCTTCAACTTCGATTACGGTTACTGGGATAGAAACGCCGTCTTCTGTGAAGATACGGGTCATCCCCACTTTTTTAc                                                                >  2:819092/1‑91 (MQ=255)            gAGTAACGCGGTTTGCTTCAACTTCGATTACGGTTACTGGGATAGAAACGCCGTCTTCTGTGAAGATACGGGTCATCCCCACTTTTTTAcc                                                               >  1:260804/1‑91 (MQ=255)            gAGTAACGCGGTTTGCTTCAACTTCGATTACGGTTACTGGGATAGAAACGCCGTCTTCTGTGAAGATACGGGTCATCCCCACTTTTTTAcc                                                               >  2:326163/1‑91 (MQ=255)                      gTTTGCTTCAACTTCGATTACGGTTACTGGGATAGAAACGCCGTCTTCTGTGAAGATACGGGTCATCCCCACTTTTTTACCGACTAAACCa                                                     >  1:819934/1‑91 (MQ=255)                        ttGCTTCAACTTCGATTACGGTTACTGGGATAGAAACGCCGTCTTCTGTGAAGATACGGGTCATCCCCACTTTTTTACCGACTAAACCAAt                                                   >  2:446415/1‑91 (MQ=255)                                aCTTCGATTACGGTTACTGGGATAGAAACGCCGTCTTCTGTGAAGATACGGGTCATCCCCACTTTTTTACCGACTAAACCAATCATTGttt                                           >  1:327209/1‑91 (MQ=255)                                  ttCGATTACGGTTACTGGGATAGAAACGCCGTCTTCTGTGAAGATACGGGTCATCCCCACTTTTTTACCGACTAAACCAATCATTGTTTCa                                         <  1:798811/91‑1 (MQ=255)                                             ttACTGGGATAGAAACGCCGTCTTCTGTGAAGATACGGGTCATCCCCACTTTTTTACCGACTAAACCAATCATTGTTTCAACCTCTCAATc                              <  1:379426/91‑1 (MQ=255)                                              tACTGGGATAGAAACGCCGTCTTCTGTGAAGATACGGGTCATCCCCACTTTTTTACCGACTAAACCAATCATTGTTTCAACCTCTCAATCg                             >  1:252812/1‑91 (MQ=255)                                              tACTGGGATAGAAACGCCGTCTTCTGTGAAGATACGGGTCATCCCCACTTTTTTACCGACTAAACCAATCATTGTTTCAACCTCTCAATCg                             <  2:811760/91‑1 (MQ=255)                                                   ggATAGAAACGCCGTCTTCTGTGAAGATACGGGTCATCCCCACTTTTTTACCGACTAAACCAATCATTGTTTCAACCTCTCAATCGCTCaa                        <  1:211474/91‑1 (MQ=255)                                                      tAGAAACGCCGTCTTCTGTGAAGATACGGGTCATCCCCACTTTTTTACCGACTAAACCAATCATTGTTTCAACCTCTCAATCGCTCAATGa                     >  1:250036/1‑91 (MQ=255)                                                        gAAGCGCCGTCTTCTGTGAAGATACGGGTCATCCCCACTTTTTTACCGACTAAACCAATCATTGTTTCAACCTCTCAATCGCTCAATGAcc                   >  1:813072/1‑91 (MQ=255)                                                             gCCGTCTTCTGTGAAGATACGGGTCATCCCCACTTTTTTACCGACTAAACCAATCATTGTTTCAACCTCTCAATCGCTCAATGACCTGAtt              <  2:28079/91‑1 (MQ=255)                                                             gCCGTCTTCTGTGAAGATACGGGTCATCCCCACTTTTTTACCGACTAAACCAATCATTGTTTCAACCTCTCAATCGCTCAATGACCTGAtt              <  1:774531/91‑1 (MQ=255)                                                                 tcttctGTGAAGATACGGGTCATCCCCACTTTTTTACCGACTAAACCAATCATTGTTTCAACCTCTCAATCGCTCAATGACCTGATTAAcc          <  2:252812/91‑1 (MQ=255)                                                                 tcttctGTGAAGATACAGGTCATCCCCACTTTTTTACCGACTAAACCAATCATTGTTTCAACCTCTCAATCGCTCAATGACCTGATTAAcc          <  1:413299/91‑1 (MQ=255)                                                                         gAAGATACGGGTCATCCCCACTTTTTTACCGACTAAACCAATCATTGTTTCAACCTCTCAATCGCTCAATGACCTGATTAACCCAGGCTGa  <  1:819092/91‑1 (MQ=255)                                                                                        |                                                                            GTCTTTAACCTGAGTAACGCGGTTTGCTTCAACTTCGATTACGGTTACTGGGATAGAAACGCCGTCTTCTGTGAAGATACGGGTCATACCCACTTTTTTACCGACTAAACCAATCATTGTTTCAACCTCTCAATCGCTCAATGACCTGATTAACCCAGGCTGA  >  NZ_CP009273/3446169‑3446331

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑