Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,479,155	A→G	V8A (GTC→GCC)	yhfA ←	OsmC family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,479,155	0	A	G	100.0%	78.9 / NA	22	V8A (GTC→GCC)	yhfA	OsmC family protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (10/12);  total (10/12)

TGGTGCTTTATCGCCTGAGTTGCCGTCCATTAAAATCTGATGACCAGAGGCGGATTCGCCCAGAAAAGTTAACCCTTCGACCCACTTCACTCGCGCTTGCATTTTTGCTACTCCACTGCGTCAATTTTCCTGACAGAGTACGCGTACTAACCAAATCGCGCAACG  >  NZ_CP009273/3479076‑3479240                                                                                |                                                                                      tGGTGCTTTATCGCCTGAGTTGCCGTCCATTAAAATCTGATGACCAGAGGCGGATTCGCCCAGAAAAGTTAACCCTTCGGCCCACTTCACt                                                                            >  1:359362/1‑91 (MQ=255) tGGTGCTTTATCGCCTGAGTTGCCGTCCATTAAAATCTGATGACCAGAGGCGGATTCGCCCAAAAAAGTTAACCCTTCGGCCCACTTCACt                                                                            >  1:88307/1‑91 (MQ=255)             gCCTGAGTTGCCGTCCATTAAAATCTGATGACCAGAGGCGGATTCGCCCAGAAAAGTTAACCCTTCGGCCCACTTCACTCGCGCTTGCAtt                                                                >  1:668124/1‑91 (MQ=255)              ccTGAGTTGCCGTCCATTAAAATCTGATGACCAGAGGCGGATTCGCCCAGAAAAGTTAACCCTTCGGCCCACTTCACTCGCGCTTGCAttt                                                               <  2:830240/91‑1 (MQ=255)               cTGAGTTGCCGTCCATTAAAATCTGATGACCAGAGGCGGATTCGCCCAGAAAAGTTAACCCTTCGGCCCACTTCACTCGCGCTTGCAtttt                                                              >  2:682643/1‑91 (MQ=255)                tGAGTTGCCGTCCATTAAAATCTGATGACCAGAGGCGGATTCGCCCAGAAAAGTTAACCCTTCGGCCCACTTCACTCGCGCTTGCAttttt                                                             <  1:200694/91‑1 (MQ=255)                tGAGTTGCCGTCCATTAAAATCTGATGACCAGAGGCGGATTCGCCCAGAAAAGTTAACCCTTCGGCCCACTTCACTCGCGCTTGCAttttt                                                             <  2:769716/91‑1 (MQ=255)                tGAGTTGCCGTCCATTAAAATCTGATGACCAGAGGCGGATTCGCCCAGAAAAGTTAACCCTTCGGCCCACTTCACTCGCGCTTGCAttttt                                                             <  2:466407/91‑1 (MQ=255)                tGAGTTGCCGTCCATTAAAATCTGATGACCAGAGGCGGATTCGCCCAGAAAAGTTAACCCTTCGGCCCACTTCACTCGCGCTTGCAttttt                                                             <  2:459808/91‑1 (MQ=255)                tGAGTTGCCGTCCATTAAAATCTGATGACCAGAGGCGGATTCGCCCAGAAAAGTTAACCCTTCGGCCCACTTCACTCGCGCTTGCAttttt                                                             <  2:111521/91‑1 (MQ=255)                 gAGTTGCCGTCCATTAAAATCTGATGACCAGAGGCGGATTCGCCCAGAAAAGTTAACCCTTCGGCCCACTTCACTCGCGCTTGCATTTTTg                                                            >  1:403750/1‑91 (MQ=255)                  aGTTGCCGTCCATTAAAATCTGATGACCAGAGGCGGATTCGCCCAGAAAAGTTAACCCTTCGGCCCACTTCACTCGCGCTTGCATTTTTGc                                                           >  1:591449/1‑91 (MQ=255)                  aGTTGCCGTCCATTAAAATCTGATGACCAGAGGCGGATTCGCCCAGAAAAGTTAACCCTTCGGCCCACTTCACTCGCGCTTGCATTTTTGc                                                           <  2:218489/91‑1 (MQ=255)                                       gatgaCCAGAGGCGGATTCGCCCAGAAAAGTTAACCCTTCGGCCCACTTCACTCGCGCTTGCATTTTTGCTACTCCACTGCGTCAATTTTc                                      >  1:323201/1‑91 (MQ=255)                                           aCCAGAGGCGGATTCGCCCAGAAAAGTTAACCCTTCGGCCCACTTCACTCGCGCTTGCATTTTTGCTACTCCACTGCGTCAATTTTCCTGa                                  <  2:343277/91‑1 (MQ=255)                                            ccAGAGGCGGATTCGCCCAGAAAAGTTAACCCTTCGGCCCACTTCACTCGCGCTTGCATTTTTGCTACTCCACTGCGTCAATTTTCCTGAc                                 <  1:54034/91‑1 (MQ=255)                                               gagGCGGATTCGCCCAGAAAAGTTAACCCTTCGGCCCACTTCACTCGCGCTTGCATTTTTGCTACTCCACTGCGTCAATTTTCCTGACaga                              <  1:761757/91‑1 (MQ=255)                                               gagGCGGATTCGCCCAGAAAAGTTAACCCTTCGGCCCACTTCACTCGCGCTTGCATTTTTGCTACTCCACTGCGTCAATTTTCCTGACaga                              <  1:341166/91‑1 (MQ=255)                                                 ggCGGATTCGCCCAGAAAAGTTAACCCTTCGGCCCACTTCACTCGCGCTTGCATTTTTGCTACTCCACTGCGTCAATTTTCCTGACAGAGt                            >  1:461114/1‑91 (MQ=255)                                                                     ttAACCCTTCGGCCCACTTCACTCGCGCTTGCATTTTTGCTACTCCACTGCGTCAATTTTCCTGACAGAGTACGCGTACTAACCAAATcgc        >  1:113558/1‑91 (MQ=255)                                                                          ccTTCGGCCCACTTCACTCGCGCTTGCATTTTTGCTACTCCACTGCGTCAATTTTCCTGACAGAGTACGCGTACTAACCAAATCGCGCAAc   <  1:219421/91‑1 (MQ=255)                                                                           cTTCGGCCCACTTCACTCGCGCTTGCATTTTTGCTACTCCACTGCGTCAATTTTCCTGACAGAGTACGCGTACTAACCAAATCGCGCAACg  >  1:751495/1‑91 (MQ=255)                                                                                |                                                                                      TGGTGCTTTATCGCCTGAGTTGCCGTCCATTAAAATCTGATGACCAGAGGCGGATTCGCCCAGAAAAGTTAACCCTTCGACCCACTTCACTCGCGCTTGCATTTTTGCTACTCCACTGCGTCAATTTTCCTGACAGAGTACGCGTACTAACCAAATCGCGCAACG  >  NZ_CP009273/3479076‑3479240

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑