| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 3,516,705 | T→C | I159T (ATC→ACC) | mrcA → | peptidoglycan glycosyltransferase/peptidoglycan DD‑transpeptidase MrcA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,516,705 | 0 | T | C | 100.0% | 44.6 / NA | 13 | I159T (ATC→ACC) | mrcA | peptidoglycan glycosyltransferase/peptidoglycan DD‑transpeptidase MrcA |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (8/5); total (8/5) | |||||||||||
GAACGCACGCTGATGCGTAAGATTAAGGAAGTCTTCCTCGCGATTCGCATTGAACAGCTGCTGACGAAAGACGAGATCCTCGAGCTTTATCTGAACAAGATTTACCTTGGTTACCGCGCCTATGGTGTCGGTGCTGCGGCACAAGTCTATTTCGGAAAAA > NZ_CP009273/3516629‑3516788 | gAACGCACGCTGATGCGTAAGATTAAGGAAGTCTTCCTCGCGATTCGCATTGAACAGCTGCTGACGAAAGACGAGACCCTCAAGCTTTATc < 1:455114/91‑1 (MQ=255) cacgcTGATGCGTAAGATTAAGGAAGTCTTCCTCGCGATTCGCATTGAACAGCTGCTGACGAAAGACGAGACCCTCGAGCTTTATCTGAAc > 1:8063/1‑91 (MQ=255) cacgcTGATGCGTAAGATTAAGGAAGTCTTCCTCGCGATTCGCATTGAACAGCTGCTGACGAAAGACGAGACCCTCGAGCTTTATCTGAAc > 2:118427/1‑91 (MQ=255) cacgcTGATGCGTAAGATTAAGGAAGTCTTCCTCGCGATTCGCATTGAACAGCTGCTGACGAAAGACGAGACCCTCGAGCTTTATCTGAAc > 2:507590/1‑91 (MQ=255) cGTAAGATTAAGGAAGTCTTCCTCGCGATTCGCATTGAACAGCTGCTGACGAAAGACGAGACCCTCGAGCTTTATCTGAACAAGATTTAcc > 2:192910/1‑91 (MQ=255) aTTAAGGAAGTCTTCCTCGCGATTCGCATTGAACAGCTGCTGACGAAAGACGAGACCCTCGAGCTTTATCTGAACAAGATTTACCTTGGtt > 2:353961/1‑91 (MQ=255) aaGGAAGTCTTCCTCGCGATTCGCATTGAACAGCTGCTGACGAAAGACGAGACCCTCGAGCTTTATCTGAACAAGATTTACCTTGGTTAcc < 1:346089/91‑1 (MQ=255) aaGGAAGTCTTCCTCGCGATTCGCATTGAACAGCTGCTGACGAAAGACGAGACCCTCGAGCTTTATCTGAACAAGATTTACCTTGGTTAcc < 2:716826/91‑1 (MQ=255) aGGAAGTCTTCCTCGCGATTCGCATTGAACAGCTGCTGACGAAAGACGAGACCCTCGAGCTTTATCTGAACAAGATTTACCTTGGTTACcg > 2:851144/1‑91 (MQ=255) gAAGTCTTCCTCGCGATTCGCATTGAACAGCTGCTGACGAAAGACGAGACCCTCGAGCTTTATCTGAACAAGATTTACCTTGGTTACcgcg < 1:212140/91‑1 (MQ=255) gTCTTCCTCGCGATTCGCATTGAACAGCTGCTGACGAAAGACGAGACCCTCGAGCTTTATCTGAACAAGATTTACCTTGGTTACCGCGCCt > 2:423271/1‑91 (MQ=255) cgcgATTCGCATTGAACAGCTGCTGACGAAAGACGAGACCCTCGAGCTTTATCTGAACAAGATTTACCTTGGTTACCGCGCCTATGGTGTc < 2:729875/91‑1 (MQ=255) gACGAGACCCTCGAGCTTTATCTGAACAAGATTTACCTTGGTTACCGCGCCTATGGTGTCGGTGCTGCGGCACAAGTCTATTTCGGaaaaa > 1:869849/1‑91 (MQ=255) | GAACGCACGCTGATGCGTAAGATTAAGGAAGTCTTCCTCGCGATTCGCATTGAACAGCTGCTGACGAAAGACGAGATCCTCGAGCTTTATCTGAACAAGATTTACCTTGGTTACCGCGCCTATGGTGTCGGTGCTGCGGCACAAGTCTATTTCGGAAAAA > NZ_CP009273/3516629‑3516788 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |