Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,526,544	C→T	A123V (GCT→GTT)	pckA →	phosphoenolpyruvate carboxykinase (ATP)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,526,544	0	C	T	100.0%	28.4 / NA	9	A123V (GCT→GTT)	pckA	phosphoenolpyruvate carboxykinase (ATP)
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (4/5);  total (4/5)

CTCTCCGGAAACCTGGCAGCATCTGAAAGGCCTGGTGACCAGGCAGCTTTCCGGCAAACGTCTGTTCGTTGTCGACGCTTTCTGTGGTGCGAACCCGGATACTCGTCTTTCCGTCCGTTTCATCACCGAAGTGGCCTGGCAGGCGCATTTT  >  NZ_CP009273/3526467‑3526617                                                                              |                                                                          ctctcCGGAAACCTGGCAGCATCTGAAAGGCCTGGTGACCAGGCAGCTTTCCGGCAAACGTCTGTTCGTTGTCGACGTTTTCTGTGGTGCg                                                              >  1:105/1‑91 (MQ=255)      cGGAAACCTGGCAGCATCTGAAAGGCCTGGTGACCAGGCAGCTTTCCGGCAAACGTCTGTTCGTTGTCGACGTTTTCTGTGGTGCGAAccc                                                         >  1:204401/1‑91 (MQ=255)           aCCTGGCAGCATCTGAAAGGCCTGGTGACCAGGCAGCTTTCCGGCAAACGTCTGTTCGTTGTCGACGTTTTCTGTGGTGCGAACCCGGATa                                                    >  2:382323/1‑91 (MQ=255)             cTGGCAGCATCTGAAAGGCCTGGTGACCAGGCAGCTTTCCGGCAAACGTCTGTTCGTTGTCGACGTTTTCTGTGGTGCGAACCCGGATACt                                                  >  1:826519/1‑91 (MQ=255)                             ggCCTGGTGACCAGGCAGCTTTCCGGCAAACGTCTGTTCGTTGTCGACGTTTTCTGTGGTGCGAACCCGGATACTCGTCTTTCCGTCCGtt                                  <  2:225024/91‑1 (MQ=255)                                    tGACCAGGCAGCTTTCCGGCAAACGTCTGTTCGTTGTCGACGTTTTCTGTGGTGCGAACCCGGATACTCGTCTTTCCGTCCGTTTCATCAc                           <  1:579449/91‑1 (MQ=255)                                         aGGCAGCTTTCCGGCAAACGTCTGTTCGTTGTCGACGTTTTCTGTGGTGCGAACCCGGATACTCGTCTTTCCGTCCGTTTCATCACCGAAg                      <  2:53794/91‑1 (MQ=255)                                           gCAGCTTTCCGGCAAACGTCTGTTCGTTGTCGACGTTTTCTGTGGTGCGAACCCGGATACTCGTCTTTCCGTCCGTTTCATCACCGAAGTg                    <  1:669841/91‑1 (MQ=255)                                                             tCTGTTCGTTGTCGACGTTTTCTGTGGTGCGAACCCGGATACTCGTCTTTCCGTCCGTTTCATCACCGAAGTGGCCTGGCAGGCGCAtttt  <  1:396816/91‑1 (MQ=255)                                                                              |                                                                          CTCTCCGGAAACCTGGCAGCATCTGAAAGGCCTGGTGACCAGGCAGCTTTCCGGCAAACGTCTGTTCGTTGTCGACGCTTTCTGTGGTGCGAACCCGGATACTCGTCTTTCCGTCCGTTTCATCACCGAAGTGGCCTGGCAGGCGCATTTT  >  NZ_CP009273/3526467‑3526617

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑