Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	453,912	A→G	E344G (GAA→GGA)	clpX →	ATP‑dependent protease ATP‑binding subunit ClpX

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	453,912	0	A	G	100.0%	112.7 / NA	30	E344G (GAA→GGA)	clpX	ATP‑dependent protease ATP‑binding subunit ClpX
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (18/12);  total (18/12)

AACTGAGCGAAGAAGCTCTGATTCAGATCCTCAAAGAGCCGAAAAACGCCCTGACCAAGCAGTATCAGGCGCTGTTTAATCTGGAAGGCGTGGATCTGGAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGGCGCGTAAAACCGGTGCCCGTGGCC  >  NZ_CP009273/453828‑453995                                                                                     |                                                                                    aaCTGAGCGAAGAAGCTCTGATTCAGATCCTCAAAGAGCCGAAAAACGCCCTGACCAAGCAGTATCAGGCGCTGTTTAATCTGGGAGGCGt                                                                               <  2:36717/91‑1 (MQ=255)    tGAGCGAAGAAGCTCTGATTCAGATCCTCAAAGAGCCGAAAAACGCCCTGACCAAGCAGTATCAGGCGCTGTTTAATCTGGGAGGCGTGGa                                                                            <  2:500283/91‑1 (MQ=255)    tGAGCGAAGAAGCTCTGATTCAGATCCTCAAAGAGCCGAAAAACGCCCTGACCAAGCAGTATCAGGCGCTGTTTAATCTGGGAGGCGTGGa                                                                            <  2:332787/91‑1 (MQ=255)         gaagaaGCTCTGATTCAGATCCTCAAAGAGCCGAAAAACGCCCTGACCAAGCAGTATCAGGCGCTGTTTAATCTGGGAGGCGTGGATCTgg                                                                       <  1:186337/91‑1 (MQ=255)          aagaagCTCTGATTGAGATCCTCAAAGAGCCGAAAAACGCCCTGACCAAGCAGTATCAGGCGCTGTTTAATCTGGGAGGCGTGGATCTGGa                                                                      >  1:746192/1‑91 (MQ=255)               gCTCTGATTCAGATCCTCAAAGAGCCGAAAAACGCCCTGACCAAGCAGTATCAGGCGCTGTTTAATCTGGGAGGCGTGGATCTGGAATTcc                                                                 >  1:219371/1‑91 (MQ=255)                          gATCCTCAAAGAGCCGAAAAACGCCCTGACCAAGCAGTATCAGGCGCTGTTTAATCTGGGAGGCGTGGATCTGGAATTCCGTGACGAGgcg                                                      >  1:107125/1‑91 (MQ=255)                          gATCCTCAAAGAGCCGAAAAACGCCCTGACCAAGCAGTATCAGGCGCTGTTTAATCTGGGAGGCGTGGATCTGGAATTCCGTGACGAGgcg                                                      >  2:493605/1‑91 (MQ=255)                           aTCCTCAAAGAGCCGAAAAACGCCCTGACCAAGCAGTATCAGGCGCTGTTTAATCTGGGAGGCGTGGATCTGGAATTCCGTGACGAGgcgc                                                     <  2:815115/91‑1 (MQ=255)                              cTCAAAGAGCCGAAAAACGCCCTGACCAAGCAGTATCAGGCGCTGTTTAATCTGGGAGGCGTGGATCTGGAATTCCGTGACGAGGCGCTgg                                                  >  2:75025/1‑91 (MQ=255)                                cAAAGAGCCGAAAAACGCCCTGACCAAGCAGTATCAGGCGCTGTTTAATCTGGGAGGCGTGGATCTGGAATTCCGTGACGAGGCGCTGGAt                                                <  1:594207/91‑1 (MQ=255)                                          aaaacCGCCCTGACCAAGCAGTATCAGGCGCTGTTTAATCTGGGAGGCGTGGATCTGGAATTCCGTGACGAGGCGCTGGATGCTATCGCTa                                      <  1:855004/91‑1 (MQ=255)                                                     gACCAAGCAGTATCAGGCGCTGTTTAATCTGGGAGGCGTGGATCTGGAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATg                           >  2:510446/1‑91 (MQ=255)                                                       ccAAGCAGTATCAGGCGCTGTTTAATCTGGGAGGCGTGGATCTGGAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGgc                         <  2:499747/91‑1 (MQ=255)                                                            cAGTATCAGGCGCTGTTTAATCTGGGAGGCGTGGATCTGGAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGGCGCGTa                    <  1:165849/91‑1 (MQ=255)                                                              gTATCAGGCGCTGTTTAATCTGGGAGGCGTGGATCTGGAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGGCGCGTaaa                  >  2:848778/1‑91 (MQ=255)                                                               tATCAGGCGCTGTTTAATCTGGGAGGCGTGGATCTGGAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGGCGCGTaaaa                 >  1:72233/1‑91 (MQ=255)                                                               tATCAGGCGCTGTTTAATCTGGGAGGCGTGGATCTGGAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGGCGCGTaaaa                 >  2:382511/1‑91 (MQ=255)                                                               tATCAGGCGCTGTTTAATCTGGGAGGCGTGGATCTGGAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGGCGCGTaaaa                 >  2:431501/1‑91 (MQ=255)                                                               tATCAGGCGCTGTTTAATCTGGGAGGCGTGGATCTGGAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGGCGCGTaaaa                 >  1:594879/1‑91 (MQ=255)                                                               tATCAGGCGCTGTTTAATCTGGGAGGCGTGGATCTGGAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGGCGCGTaaaa                 >  1:216362/1‑91 (MQ=255)                                                               tATCAGGCGCTGTTTAATCTGGGAGGCGTGGATCTGGAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGGCGCGTaaaa                 >  2:868057/1‑91 (MQ=255)                                                                    ggCGCTGTTTAATCTGGGAGGCGTGGATCTGGAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGGCGCGTAAAACCGGt            <  1:868057/91‑1 (MQ=255)                                                                    ggCGCTGTTTAATCTGGGAGGCGTGGATCTGGAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGGCGCGTAAAACCGGt            >  1:433542/1‑91 (MQ=255)                                                                        cTGTTTAATCTGGGAGGCGTGGATCTGGAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGGCGCGTAAAACCGGTGccc        >  2:340469/1‑91 (MQ=255)                                                                        cTGTTTAATCTGGGAGGCGTGGATCTGGAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGGCGCGTAAAACCGGTGccc        >  1:625349/1‑91 (MQ=255)                                                                        cTGTTTAATCTGGGAGGCGTGGATCTGGAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGGCGCGTAAAACCGGTGccc        >  1:238026/1‑91 (MQ=255)                                                                          gTTTAATCTGGGAGGCGTGGATCTGGAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGGCGCGTAAAACCGGTGCCCGt      >  1:827088/1‑91 (MQ=255)                                                                           tttAATCTGGGAGGCGTGGATCTGGAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGGCGCGTAAAACCGGTGCCCGTg     <  1:300635/91‑1 (MQ=255)                                                                              aaTCTGGGAGGCGTGGATCTGGAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGGCGCGTAAAACCGGTGCCCGTGGcc  <  1:497426/91‑1 (MQ=255)                                                                                     |                                                                                    AACTGAGCGAAGAAGCTCTGATTCAGATCCTCAAAGAGCCGAAAAACGCCCTGACCAAGCAGTATCAGGCGCTGTTTAATCTGGAAGGCGTGGATCTGGAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGGCGCGTAAAACCGGTGCCCGTGGCC  >  NZ_CP009273/453828‑453995

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑