Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,836,431	A→G	V240A (GTC→GCC)	adeQ ←	adenine permease AdeQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,836,431	0	A	G	100.0%	147.2 / NA	39	V240A (GTC→GCC)	adeQ	adenine permease AdeQ
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (21/18);  total (21/18)

TGAATCAAATAGGTTGATCAGCATAAAGGAGAAAATGATACCGGCGAGTTCAAGTGTTAACGCGCCGCTCAAATCTACTTCACCAATGACGCCGCTAATATCAGGCGGAATGGAATAGACGCCGCTAAAATGAACATCACCGAAAAATAATCCACAGCAAGACGTCACCACAATAG  >  NZ_CP009273/3836343‑3836518                                                                                         |                                                                                        tgaATCAAATAGGTTGATCAGCATAAAGGAGAAAATGATACCGGCGAGTTCAAGTGTTAACGCGCCGCTCAAATCTACTTCACCAATGGcg                                                                                       <  1:226080/91‑1 (MQ=255)    aTCAAATAGGTTGATCAGCATAAAGGAGAAAATGATACCGGCGAGTTCAAGTGTTAACGCGCCGCTCAAATCTACTTCACCAATGGcgccg                                                                                    >  1:701817/1‑91 (MQ=255)            ggTTGATCAGCATAAAGGAGAAAATGATACCGGCGAGTTCAAGTGTTAACGCGCCGCTCAAATCTACTTCACCAATGGCGCCGCTAATATc                                                                            >  2:11329/1‑91 (MQ=255)              ttGATCAGCATAAAGGAGAAAATGATACCGGCGAGTTCAAGTGTTAACGCGCCGCTCAAATCTACTTCACCAATGGCGCCGCTAATATCAg                                                                          >  1:345900/1‑91 (MQ=255)                gATCAGCATAAAGGAGAAAATGATACCGGCGAGTTCAAGTGTTAACGCGCCGCTCAAATCTACTTCACCAATGGCGCCGCTAATATCAGGc                                                                        >  1:25650/1‑91 (MQ=255)                gATCAGCATAAAGGAGAAAATGATACCGGCGAGTTCAAGTGTTAACGCGCCGCTCAAATCTACTTCACCAATGGCGCCGCTAATATCAGGc                                                                        <  2:807741/91‑1 (MQ=255)                gATCAGCATAAAGGAGAAAATGATACCGGCGAGTTCAAGTGTTAACGCGCCGCTCAAATCTACTTCACCAATGGCGCCGCTAATATCAGGc                                                                        >  2:585223/1‑91 (MQ=255)                gATCAGCATAAAGGAGAAAATGATACCGGCGAGTTCAAGTGTTAACGCGCCGCTCAAATCTACTTCACCAATGGCGCCGCTAATATCAGGc                                                                        <  1:585223/91‑1 (MQ=255)                  tCAGCATAAAGGAGAAAATGATACCGGCGAGTTCAAGTGTTAACGCGCCGCTCAAATCTACTTCACCAATGGCGCCGCTAATATCAGGCgg                                                                      <  2:553652/91‑1 (MQ=255)                     gCATAAAGGAGAAAATGATACCGGCGAGTTCAAGTGTTAACGCGCCGCTCAAATCTACTTCACCAATGGCGCCGCTAATATCAGGCggaat                                                                   >  2:257966/1‑91 (MQ=255)                              agaAAATGATACCGGCGAGTTCAAGTGTTAACGCGCCGCTCAAATCTACTTCACCAATGGCGCCGCTAATATCAGGCGGAATGGAATAGAc                                                          <  1:520555/91‑1 (MQ=255)                              agaAAATGATACCGGCGAGTTCAAGTGTTAACGCGCCGCTCAAATCTACTTCACCAATGGCGCCGCTAATATCAGGCGGAATGGAATAGAc                                                          <  1:675997/91‑1 (MQ=255)                               gaAAATGATACCGGCGAGTTCAAGTGTTAACGCGCCGCTCAAATCTACTTCACCAATGGCGCCGCTAATATCAGGCGGAATGGAATAGAcg                                                         >  2:436917/1‑91 (MQ=255)                               gaAAATGATACCGGCGAGTTCAAGTGTTAACGCGCCGCTCAAATCTACTTCACCAATGGCGCCGCTAATATCAGGCGGAATGGAATAGAcg                                                         >  2:69698/1‑91 (MQ=255)                                    tGATACCGGCGAGTTCAAGTGTTAACGCGCCGCTCAAATCTACTTCACCAATGGCGCCGCTAATATCAGGCGGAATGGAATAGACGCCGCt                                                    >  1:563337/1‑91 (MQ=255)                                     gATACCGGCGAGTTCAAGTGTTAACGCGCCGCTCAAATCTACTTCACCAATGGCGCCGCTAATATCAGGCGGAATGGAATAGACGCCGCTa                                                   <  1:436917/91‑1 (MQ=255)                                      aTACCGGCGAGTTCAAGTGTTAACGCGCCGCTCAAATCTACTTCACCAATGGCGCCGCTAATATCAGGCGGAATGGAATAGACGCCGCTaa                                                  <  2:90160/91‑1 (MQ=255)                                           ggCGAGTTCAAGTGTTAACGCGCCGCTCAAATCTACTTCACCAATGGCGCCGCTAATATCAGGCGGAATGGAATAGACGCCGCTAAAATGa                                             >  2:264118/1‑91 (MQ=255)                                           ggCGAGTTCAAGTGTTAACGCGCCGCTCAAATCTACTTCACCAATGGCGCCGCTAATATCAGGCGGAATGGAATAGACGCCGCTAAAATGa                                             >  1:134051/1‑91 (MQ=255)                                           ggCGAGTTCAAGTGTTAACGCGCCGCTCAAATCTACTTCACCAATGGCGCCGCTAATATCAGGCGGAATGGAATAGACGCCGCTAAAATGa                                             >  2:585606/1‑91 (MQ=255)                                           ggCGAGTTCAAGTGTTAACGCGCCGCTCAAATCTACTTCACCAATGGCGCCGCTAATATCAGGCGGAATGGAATAGACGCCGCTAAAATGa                                             >  1:615811/1‑91 (MQ=255)                                                gTTCAAGTGTTAACGCGCCGCTCAAATCTACTTCACCAATGGCGCCGCTAATATCAGGCGGAATGGAATAGACGCCGCTAAAATGAACATc                                        <  1:366416/91‑1 (MQ=255)                                                          tAACGCGCCGCTCAAATCTACTTCACCAATGGCGCCGCTAATATCAGGCGGAATGGAATAGAcgccgc                                                     <  1:109423/68‑1 (MQ=255)                                                          tAACGCGCCGCTCAAATCTACTTCACCAATGGCGCCGCTAATATCAGGCGGAATGGAATAGAcgccgc                                                     >  2:109423/1‑68 (MQ=255)                                                          tAACGCGCCGCTCAAATCTACTTCACCAATGGCGCCGCTAATATCAGGCGGAATGGAATAGACGCCGCTAAAATGAACATCACCGAAAaat                              >  2:737207/1‑91 (MQ=255)                                                          tAACGCGCCGCTCAAATCTACTTCACCAATGGCGCCGCTAATATCAGGCGGAATGGAATAGACGCCGCTAAAATGAACATCACCGAAAaat                              >  2:62193/1‑91 (MQ=255)                                                                gccgcTCAAATCTACTTCACCAATGGCGCCGCTAATATCAGGCGGAATGGAATAGACGCCGCTAAAATGAACATCACCGAAAAATAATCca                        >  2:530329/1‑91 (MQ=255)                                                                  cgcTCAAATCTACTTCACCAATGGCGCCGCTAATATCAGGCGGAATGGAATAGACGCCGCTAAAATGAACATCACCGAAAAATAATCcaca                      >  2:185780/1‑91 (MQ=255)                                                                  cgcTCAAATCTACTTCACCAATGGCGCCGCTAATATCAGGCGGAATGGAATAGACGCCGCTAAAATGAACATCACCGAAAAATAATCcaca                      >  2:168682/1‑91 (MQ=255)                                                                       aaaTCTACTTCACCAATGGCGCCGCTAATATCAGGCGGAATGGAATAGACGCCGCTAAAATGAACATCACCGAAAAATAATCCACAGCAAg                 <  1:49304/91‑1 (MQ=255)                                                                          tCTACTTCACCAATGGCGCCGCTAATATCAGGCGGAATGGAATAGACGCCACTAAAACGAACATCACCGAAAAATAATCCACAGCAAGACg              <  2:660087/91‑1 (MQ=255)                                                                            tACTTCACCAATGGCGCCGCTAATATCAGGCGGAATGGAATAGACGCCGCTAAAATGAACATCACCGAAAAATAATCCACAGCAAGACGTc            <  1:551413/91‑1 (MQ=255)                                                                            tACTTCACCAATGGCGCCGCTAATATCAGGCGGAATGGAATAGACGCCGCTAAAATGAACATCACCGAAAAATAATCCACAGCAAGACGTc            <  2:484230/91‑1 (MQ=255)                                                                            tACTTCACCAATGGCGCCGCTAATATCAGGCGGAATGGAATAGACGCCGCTAAAATGAACATCACCGAAAAATAATCCACAGCAAGACGTc            <  2:28014/91‑1 (MQ=255)                                                                                tCACCAATGGCGCCGCTAATATCAGGCGGAATGGAATAGACGCCGCTAAAATGAACATCACCGAAAAATAATCCACAGCAAGACGTcacca        >  1:539406/1‑91 (MQ=255)                                                                                  aCCAATGGCGCCGCTAATATCAGGCGGAATGGAATAGACGCCGCTAAAATGAACATCACCGAAAAATAATCCACAGCAAGACGTCACcaca      >  1:351769/1‑91 (MQ=255)                                                                                    cAATGGCGCCGCTAATATCAGGCGGAATGGAATAGACGCCGCTAAAATGAACATCACCGAAAAATAATCCACAGCAAGACGTCACCACAAt    <  1:513832/91‑1 (MQ=255)                                                                                      aTGGCGCCGCTAATATCAGGCGGAATGGAATAGACGCCGCTAAAATGAACATCACCGAAAAATAATCCACAGCAAGACGTCACCACAATAg  <  2:424195/91‑1 (MQ=255)                                                                                      aTGGCGCCGCTAATATCAGGCGGAATGGAATAGACGCCGCTAAAATGAACATCACCGAAAAATAATCCACAGCAAGACGTCACCACAATAg  <  1:552678/91‑1 (MQ=255)                                                                                         |                                                                                        TGAATCAAATAGGTTGATCAGCATAAAGGAGAAAATGATACCGGCGAGTTCAAGTGTTAACGCGCCGCTCAAATCTACTTCACCAATGACGCCGCTAATATCAGGCGGAATGGAATAGACGCCGCTAAAATGAACATCACCGAAAAATAATCCACAGCAAGACGTCACCACAATAG  >  NZ_CP009273/3836343‑3836518

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑