Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,841,593	A→G	F131S (TTC→TCC)	uhpC ←	MFS transporter family glucose‑6‑phosphate receptor UhpC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,841,593	0	A	G	100.0%	133.1 / NA	36	F131S (TTC→TCC)	uhpC	MFS transporter family glucose‑6‑phosphate receptor UhpC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (25/11);  total (25/11)

ATGCCCACCAACCGCCGCGCTCGGTACGTGAATACCAGGCCGTTAACAGACGCGCACACACCGGTGAACCCCAGCCCTGGAAAAAGGCGTTCAGCACCCAGAGCACGGCAAACGCCCATAGCGACGTCGAGAAGCCAAACAGAATGTTGATAATGCCCGTGGCGATA  >  NZ_CP009273/3841513‑3841679                                                                                 |                                                                                       aTGCCCACCAACCGCCGCGCTCGGTACGTGAATACCAGGCCGTTAACAGACGCGCACACACCGGTGAACCCCAGCCCTGGGAAAAGGCGtt                                                                              >  1:315167/1‑91 (MQ=255) aTGCCCACCAACCGCCGCGCTCGGTACGTGAATACCAGGCCGTTAACAGACGCGCACACACCGGTGAACCCCAGCCCTGGGAAAAGGCGtt                                                                              >  1:661004/1‑91 (MQ=255)    cccACCAACCGCCGCGCTCGGTACGTGAATACCAGGCCGTTAACAGACGCGCACACACCGGTGAACCCCAGCCCTGGGAAAAGGCGTTCAg                                                                           <  2:611488/91‑1 (MQ=255)      caccaACCGCCGCGCTCGGTACGTGAATACCAGGCCGTTAACAGACGCGCACACACCGGTGAACCCCAGCCCTGGGAAAAGGCGTTCAGCa                                                                         >  1:812821/1‑91 (MQ=255)      caccaACCGCCGCGCTCGGTACGTGAATACCAGGCCGTTAACAGACGCGCACACACCGGTGAACCCCAGCCCTGGGAAAAGGCGTTCAGCa                                                                         >  1:309218/1‑91 (MQ=255)           aCCGCCGCGCTCGGTACGTGAATACCAGGCCGTTAACAGACGCGCACACACCGGTGAACCCCAGCCCTGGGAAAAGGCGTTCAGCACCCag                                                                    >  2:660576/1‑91 (MQ=255)                              gAATACCAGGCCGTTAACAGACGCGCACACACCGGTGAACCCCAGCCCTGGGAAAAGGCGTTCAGCACCCAGAGCACGGCAAACGCCCATa                                                 >  1:191752/1‑91 (MQ=255)                                aTACCAGGCCGTTAACAGACGCGCACACACCGGTGAACCCCAGCCCTGGGAAAAGGCGTTCAGCACCCAGAGCACGGCAAACGCCCATAGc                                               >  2:541043/1‑91 (MQ=255)                                aTACCAGGCCGTTAACAGACGCGCACACACCGGTGAACCCCAGCCCTGGGAAAAGGCGTTCAGCACCCAGAGCACGGCAAACGCCCATAGc                                               <  1:457028/91‑1 (MQ=255)                                  aCCAGGCCGTTAACAGACGCGCACACACCGGTGAACCCCAGCCCTGGGAAAAGGCGTTCAGCACCCAGAGc                                                                 <  1:509924/71‑1 (MQ=255)                                  aCCAGGCCGTTAACAGACGCGCACACACCGGTGAACCCCAGCCCTGGGAAAAGGCGTTCAGCACCCAGAGc                                                                 >  2:509924/1‑71 (MQ=255)                                  aCCAGGCCGTTAACAGACGCGCACACACCGGTGAACCCCAGCCCTGGGAAAAGGCGTTCAGCACCCAGAGCACGGCAAACGCCCATAGCGa                                             >  1:429821/1‑91 (MQ=255)                                  aCCAGGCCGTTAACAGACGCGCACACACCGGTGAACCCCAGCCCTGGGAAAAGGCGTTCAGCACCCAGAGCACGGCAAACGCCCATAGCGa                                             >  1:135488/1‑91 (MQ=255)                                     aGGCCGTTAACAGACGCGCACACACCGGTGAACCCCAGCCCTGGGAAAAGGCGTTCAGCACCCAGAGCACGGCAAACGCCCATAGCGACGt                                          >  2:520193/1‑91 (MQ=255)                                      ggCCGTTAACAGACGCGCACACACCGGTGAACCCCAGCCCTGGGAAAAGGCGTTCAGCACCCAGAGCACGGCAAACGCCCATAGCGACGTc                                         >  1:195130/1‑91 (MQ=255)                                          gTTAACAGACGCGCACACACCGGTGAACCCCAGCCCTGGGAAAAGGCGTTCAGCACCCAGAGCACGGCAAACGCCCATAGCGACGTCgaga                                     <  1:351335/91‑1 (MQ=255)                                               cAGACGCGCACACACCGGTGAACCCCAGCCCTGGGAAAAGGCGTTCAGCACCCAGAGCACGGCAAACGCCCATAGCGACGTCGAGAAGCCa                                <  1:514104/91‑1 (MQ=255)                                                 gACGCGCACACACCGGTGAACCCCAGCCCTGGGAAAAGGCGTTCAGCACCCAGAGCACGGCAAACGCCCATAGCGACGTCgaga                                     >  1:380703/1‑84 (MQ=255)                                                 gACGCGCACACACCGGTGAACCCCAGCCCTGGGAAAAGGCGTTCAGCACCCAGAGCACGGCAAACGCCCATAGCGACGTCgaga                                     <  2:380703/84‑1 (MQ=255)                                                 gACGCGCACACACCGGTGAACCCCAGCCCTGGGAAAAGGCGTTCAGCACCCAGAGCACGGCAAACGCCCATAGCGACGTCGAGAAGCCaaa                              >  1:501744/1‑91 (MQ=255)                                                 gACGCGCACACACCGGTGAACCCCAGCCCTGGGAAAAGGCGTTCAGCACCCAGAGCACGGCAAACGCCCATAGCGACGTCGAGAAGCCaaa                              >  2:685345/1‑91 (MQ=255)                                                    gcgcACACACCGGTGAACCCCAGCCCTGGGAAAAGGCGTTCAGCACCCAGAGCACGGCAAACGCCCATAGCGACGTCGAGAAGCCAAACAg                           >  2:556446/1‑91 (MQ=255)                                                      gcACACACCCGTGAACCCCAGCCCTGGGAAAAGGCGTTCAGCACCCAGAGCACGGCAAACGCCCATAGCGACGTCGAGAAGCCAAACAGaa                         >  2:307727/1‑91 (MQ=255)                                                         cacacCGGTGAACCCCAGCCCTGGGAAAAGGCGTTCAGCACCCAGAGCACGGCAAACGCCCATAGCGACGTCGAGAAGCCAAACAGAATGt                      >  2:155954/1‑91 (MQ=255)                                                         cacacCGGTGAACCCCAGCCCTGGGAAAAGGCGTTCAGCACCCAGAGCACGGCAAACGCCCATAGCGACGTCGAGAAGCCAAACAGAATGt                      <  2:191752/91‑1 (MQ=255)                                                           cacCGGTGAACCCCAGCCCTGGGAAAAGGCGTTCAGCACCCAGAGCACGGCAAACGCCCATAGCGACGTCGAGAAGCCAAACAGAATGTTg                    >  1:729686/1‑91 (MQ=255)                                                                     ccccAGCCCTGGGAAAAGGCGTTCAGCACCCAGAGCACGGCAAACGCCCATAGCGACGTCGAGAAGCCAAACAGAATGTTGATAATGCCCg          >  1:156897/1‑91 (MQ=255)                                                                     ccccAGCCCTGGGAAAAGGCGTTCAGCACCCAGAGCACGGCAAACGCCCATAGCGACGTCGAGAAGCCAAACAGAATGTTGATAATGCCCg          >  1:197334/1‑91 (MQ=255)                                                                       ccAGCCCTGGGAAAAGGCGTTCAGCACCCAGAGCACGGCAAACGCCCATAGCGACGTCGAGAAGCCAAACAGa                          >  2:255396/1‑73 (MQ=255)                                                                       ccAGCCCTGGGAAAAGGCGTTCAGCACCCAGAGCACGGCAAACGCCCATAGCGACGTCGAGAAGCCAAACAGa                          <  1:255396/73‑1 (MQ=255)                                                                       ccAGCCCTGGGAAAAGGCGTTCAGCACCCAGAGCACGGCAAACGCCCATAGCGACGTCGAGAAGCCAAACAGAATGTTGATAATGCCCGTg        >  2:564387/1‑91 (MQ=255)                                                                        cAGCCCTGGGAAAAGGCGTTCAGCACCCAGAGCACGGCAAACGCCCATAGCGACGTCGAGAAGCCAAACAGAATGTTGATAATGCCCGTgg       >  2:136872/1‑91 (MQ=255)                                                                         aGCCCTGGGAAAAGGCGTTCAGCACCCAGAGCACGGCAAACGCCCATAGCGACGTCGAGAAGCCAAACAGAATGTTGATAATGCCCGTGGc      >  2:792326/1‑91 (MQ=255)                                                                            ccTGGGAAAAGGCGTTCAGCACCCAGAGCACGGCAAACGCCCATAGCGACGTCGAGAAGCCAAACAGAATGTTGATAATGCCCGTGGCGAt   <  1:747744/91‑1 (MQ=255)                                                                             cTGGGAAAAGGCGTTCAGCACCCAGAGCACGGCAAACGCCCATAGCGACGTCGAGAAGCCAAACAGAATGTTGATAATGCCCGTGGCGATa  <  2:135488/91‑1 (MQ=255)                                                                             cTGGGAAAAGGCGTTCAGCACCCAGAGCACGGCAAACGCCCATAGCGACGTCGAGAAGCCAAACAGAATGTTGATAATGCCCGTGGCGATa  <  2:812821/91‑1 (MQ=255)                                                                                 |                                                                                       ATGCCCACCAACCGCCGCGCTCGGTACGTGAATACCAGGCCGTTAACAGACGCGCACACACCGGTGAACCCCAGCCCTGGAAAAAGGCGTTCAGCACCCAGAGCACGGCAAACGCCCATAGCGACGTCGAGAAGCCAAACAGAATGTTGATAATGCCCGTGGCGATA  >  NZ_CP009273/3841513‑3841679

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑