| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 3,882,139 | A→G | E17G (GAG→GGG) | tnaA → | tryptophanase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,882,139 | 0 | A | G | 95.8% | 84.5 / ‑6.0 | 24 | E17G (GAG→GGG) | tnaA | tryptophanase |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (15/8); minor base T (1/0); total (16/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CAGGGATCACTGTAATTAAAATAAATGAAGGATTATGTAATGGAAAACTTTAAACATCTCCCTGAACCGTTCCGCATTCGTGTTATTGAGCCAGTAAAACGTACCACTCGCGCTTATCGTGAAGAGGCAATTATTAAATCCGGTATGAACCCGTTCCTGCTGGATAGCGAAGAT > NZ_CP009273/3882051‑3882224 | caGGGATCACTGTAATTAAAATAAATGAAGGATTATGTAATGGAAAACTTTAAACATCTCCCTGAACCGTTCCGCATTCGTGTTATTGGGc > 1:560719/1‑91 (MQ=255) aGGGATCACTGTAATTAAAATAAATGAAGGATTATGTAATGGAAAACTTTAAACATCTCCCTGAACCGTTCCGCATTCGTGTTATTGGGcc < 1:169745/91‑1 (MQ=255) aGGGATCACTGTAATTAAAATAAATGAAGGATTATGTAATGGAAAACTTTAAACATCTCCCTGAACCGTTCCGCATTCGTGTTATTGGGcc < 1:265280/91‑1 (MQ=255) ggATCACTGTAATTAAAATAAATGAAGGATTATGTAATGGAAAACTTTAAACATCTCCCTGAACCGTTCCGCATTCGTGTTATTGGGCCAg > 1:302030/1‑91 (MQ=255) gATCACTGTAATTAAAATAAATGAAGGATTATGTAATGGAAAACTTTAAACATCTCCCTGAACCGTTCCGCATTCGTGTTATTGTGCCAGt > 2:725529/1‑91 (MQ=255) aCTGTAATTAAAATAAATGAAGGATTATGTAATGGAAAACTTTAAACATCTCCCTGAACCGTTCCGCATTCGTGTTATTGGGCCAGTaaaa < 2:683742/91‑1 (MQ=255) tAATTAAAATAAATGAAGGATTATGTAATGGAAAACTTTAAACATCTCCCTGAACCGTTCAGCATTCGTGTTATTGGGCCAGTAAAACGTa > 1:424478/1‑91 (MQ=255) ggATTATGTAATGGAAAACTTTAAACATCTCCCTGAACCGTTCCGCATTCGTGTTATTGGGCCAGTAAAACGTACCACTCGCGCTTATCGt < 1:470835/91‑1 (MQ=255) tAATGGAAAACTTTAAACATCTCCCTGAACCGTTCCGCATTCGTGTTATTGGGCCAGTAAAACGTACCACTCGCGCTTATCGTGAAGAGGc < 2:584395/91‑1 (MQ=255) aTGGAAAACTTTAAACATCTCCCTGAACCGTTCCGCATTCGTGTTATTGGGCCAGTAAAACGTACCACTCGCGCTTATCGTGAAGAGGCaa > 2:177723/1‑91 (MQ=255) aTGGAAAACTTTAAACATCTCCCTGAACCGTTCCGCATTCGTGTTATTGGGCCAGTAAAACGTACCACTCGCGCTTATCGTGAAGAGGCaa > 2:177722/1‑91 (MQ=255) tctcCCTGAACCGTTCCGCATTCGTGTTATTGGGCCAGTAAAACGTACCACTCGCGCTTATCGTGAAGAGGCAATTATTAAATCCGGTATg > 1:178307/1‑91 (MQ=255) ccGTTCCGCATTCGTGTTATTGGGCCAGTAAAACGTACCACTCGCGCTTATCGTGAAGAGGCAATTATTAAATCCGGTATGAACCCGTTcc < 2:589356/91‑1 (MQ=255) gTTCCGCATTCGTGTTATTGGGCCAGTAAAACGTACCACTCGCGCTTATCGTGAAGAGGCAATTATTAAATcc > 1:770782/1‑73 (MQ=255) gTTCCGCATTCGTGTTATTGGGCCAGTAAAACGTACCACTCGCGCTTATCGTGAAGAGGCAATTATTAAATcc < 2:770782/73‑1 (MQ=255) gTTCCGCATTCGTGTTATTGGGCCAGTAAAACGTACCACTCGCGCTTATCGTGAAGAGGCAATTATTAAATCCGGTATGAACCCGTTCctg > 2:219688/1‑91 (MQ=255) gTTCCGCATTCGTGTTATTGGGCCAGTAAAACGTACCACTCGCGCTTATCGTGAAGAGGCAATTATTAAATCCGGTATGAACCCGTTCctg > 2:7804/1‑91 (MQ=255) gTTCCGCATTCGTGTTATTGGGCCAGTAAAACGTACCACTCGCGCTTATCGTGAAGAGGCAATTATTAAATCCGGTATGAACCCGTTCctg > 2:867594/1‑91 (MQ=255) ttCCGCATTCGTGTTATTGGGCCAGTAAAACGTACCACTCGCGCTTATCGTGAAGAGGCAATTATTAAATCCGGTATGAACCCGTTCctgc > 2:148653/1‑91 (MQ=255) ttCCGCATTCGTGTTATTGGGCCAGTAAAACGTACCACTCGCGCTTATCGTGAAGAGGCAATTATTAAATCCGGTATGAACCCGTTCctgc > 2:30564/1‑91 (MQ=255) ttCCGCATTCGTGTTATTGGGCCAGTAAAACGTACCACTCGCGCTTATCGTGAAGAGGCAATTATTAAATCCGGTATGAACCCGTTCctgc < 2:461031/91‑1 (MQ=255) ttCCGCATTCGTGTTATTGGGCCAGTAAAACGTACCACTCGCGCTTATCGTGAAGAGGCAATTATTAAATCCGGTATGAACCCGTTCctgc > 1:69008/1‑91 (MQ=255) ttCCGCATTCGTGTTATTGGGCCAGTAAAACGTACCACTCGCGCTTATCGTGAAGAGGCAATTATTAAATCCGGTATGAACCCGTTCctgc > 1:585855/1‑91 (MQ=255) tATTGGGCCAGTAAAACGTACCACTCGCGCTTATCGTGAAGAGGCAATTATTAAATCCGGTATGAACCCGTTCCTGCTGGATAGCGAAGAt > 2:309658/1‑91 (MQ=255) | CAGGGATCACTGTAATTAAAATAAATGAAGGATTATGTAATGGAAAACTTTAAACATCTCCCTGAACCGTTCCGCATTCGTGTTATTGAGCCAGTAAAACGTACCACTCGCGCTTATCGTGAAGAGGCAATTATTAAATCCGGTATGAACCCGTTCCTGCTGGATAGCGAAGAT > NZ_CP009273/3882051‑3882224 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |