Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,896,219	A→G	T281T (ACT→ACC)	bglB ←	6‑phospho‑beta‑glucosidase BglB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,896,219	0	A	G	100.0%	139.0 / NA	37	T281T (ACT→ACC)	bglB	6‑phospho‑beta‑glucosidase BglB
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (15/22);  total (15/22)

GCTTTCGTCGTGGGAAACACAACCAGTCATGTAATAACTAAAAGAGATGAAATCGACGGTATGTTTTAAATCTTCTGCGTCACTTTCAGTCATCTCAATGGTGATATTGTGGTCGCGGAAGAAACGCTGCATATAGCCGGGATACTGGCCACGCGCCTGAACATC  >  NZ_CP009273/3896132‑3896296                                                                                        |                                                                              gCTTTCGTCGTGGGAAACACAACCAGTCATGTAATAACTAAAAGAGATGAAATCGACGGTATGTTTTAAATCTTCTGCGTCACTTTCGGTc                                                                            <  2:491509/91‑1 (MQ=255)    ttCGTCGTGGGAAACACAACCAGTCACGTAATAACTAAAAGAGATGAAATCGACGGTATGTTTTAAATCTTCTGCGTCACTTTCGGTCAtc                                                                         >  2:763388/1‑91 (MQ=255)         cgtGGGAAACACAACCAGTCATGTAATAACTAAAAGAGATGAAATCGACGGTATGTTTTAAATCTTCTGCGTCACTTTCGGTCATCTCAAt                                                                    <  2:216901/91‑1 (MQ=255)         cgtCGGAAACACAACCAGTCATGTAATAACTAAAAGAGATGAAATCGACGGTATGTTTTAAATCTTCTGCGTCACTTTCGGTCATCTCAAt                                                                    <  2:182960/91‑1 (MQ=255)          gtGGGAAACACAACCAGTCATGTAATAACTAAAAGAGATGAAATCGACGGTATGTTTTAAATCTTCTGCGTCACTTTCGGTCATCTCAATg                                                                   >  2:697523/1‑91 (MQ=255)             ggAAACACAACCAGTCATGTAATAACTAAAAGAGATGAAATCGACGGTATGTTTTAAATCTTCTGCGTCACTTTCGGTCATCTCAATAGTg                                                                <  1:768927/91‑1 (MQ=255)                    caACCAGTCATGTAATAACTAAAAGAGATGAAATCGACGGTATGTTTTAAATCTTCTGCGTCACTTTCGGTCATCTCAATGGTGATATtgt                                                         >  2:350697/1‑91 (MQ=255)                        cAGTCATGTAATAACTAAAAGAGATGAAATCGACGGTATGTTTTAAATCTTCTGCGTCACTTTCGGTCATCTCAATGGTGATATTGTGGTc                                                     <  1:672143/91‑1 (MQ=255)                         aGTCATGTAATAACTAAAAGAGATGAAATCGACGGTATGTTTTAAATCTTCTGCGTCACTTTCGGTc                                                                            <  1:653103/67‑1 (MQ=255)                         aGTCATGTAATAACTAAAAGAGATGAAATCGACGGTATGTTTTAAATCTTCTGCGTCACTTTCGGTc                                                                            >  2:653103/1‑67 (MQ=255)                           tCATGTAATAACTAAAAGAGATGAAATCGACGGTATGTTTTAAATCTTCTGCGTCACTTTCGGTCATCTCAATGGTGATATTGTGGTcgcg                                                  <  1:667841/91‑1 (MQ=255)                                taataaCTAAAAGAGATGAAATCGACGGTATGTTTTAAATCTTCTGCGTCACTTTCGGTCATCTCAATGGTGATATTGTGGTCGCGgaaga                                             <  1:74737/91‑1 (MQ=255)                                    aaCTAAAAGAGATGAAATCGACGGTATGTTTTAAATCTTCTGCGTCACTTTCGGTCATCTCAATGGTGATATTGTGGTCGCGGAAGAAACg                                         >  1:712650/1‑91 (MQ=255)                                    aaCTAAAAGAGATGAAATCGACGGTATGTTTTAAATCTTCTGCGTCACTTTCGGTCATCTCAATGGTGATATTGTGGTCGCGGAAGAAACg                                         >  2:390483/1‑91 (MQ=255)                                    aaCTAAAAGAGATGAAATCGACGGTATGTTTTAAATCTTCTGCGTCACTTTCGGTCATCTCAATGGTGATATTGTGGTCGCGGAAGAAACg                                         >  2:715/1‑91 (MQ=255)                                      cTAAAAGAGATGAAATCGACGGTATGTTTTAAATCTTCTGCGTCACTTTCGGTCATCTCAATGGTGATATTGTGGTCGCGGAAGAAACGCt                                       <  2:178133/91‑1 (MQ=255)                                       tAAAAGAGATGAAATCGACGGTATGTTTTAAATCTTCTGCGTCACTTTCGGTCATCTCAATGGTGATATTGTGGTCGCGGAAGAAACGCTg                                      <  1:715/91‑1 (MQ=255)                                        aaaaGAGATGAAATCGACGGTATGTTTTAAATCTTCTGCGTCACTTTCGGTCAtctc                                                                       >  2:599574/1‑57 (MQ=255)                                        aaaaGAGATGAAATCGACGGTATGTTTTAAATCTTCTGCGTCACTTTCGGTCAtctc                                                                       <  1:599574/57‑1 (MQ=255)                                        aaaaGAGATGAAATCGACGGTATGTTTTAAATCTTCTGCGACACTTTCGGTCATCTCAATGGTGATATTGTGGTCGCGGAAGAAACGCTGc                                     >  1:566289/1‑91 (MQ=255)                                             agaTGAAATCGACGGTATGTTTTAAATCTTCTGCGTCACTTTCGGTCATCTCAATGGTGATATTGTGGTCGCGGAAGAAACGCTGCAtata                                <  2:352928/91‑1 (MQ=255)                                              gaTGAAATCGACGGTATGTTTTAAATCTTCTGCGTCACTTTCGGTCATCTCAATGGTGATATTGTGGTCGCGGAAGAAACGCTGCATATAg                               >  2:560641/1‑91 (MQ=255)                                                tGAAATCGACGGTATGTTTTAAATCTTCTGCGTCACTTTCGGTCATCTCAATGGTGATATTGTGGTCGCGGAAGAAACGCTGCATATAGcc                             >  2:678710/1‑91 (MQ=255)                                                 gAAATCGACGGTATGTTTTAAATCTTCTGCGTCACTTTCGGTCATCTCAATGGTGATATTGTGGTCGCGGAAGAAACGCTGCATATAGCCg                            <  1:390483/91‑1 (MQ=255)                                                  aaaTCGACGGTATGTTTTAAATCTTCTGCGTCACTTTCGGTCATCTCAATGGTGATATTGTGGTCGCGGAAGAAACGCTGCATATAGCCgg                           <  1:85271/91‑1 (MQ=255)                                                              tGTTTTAAATCTTCTGCGTCACTTTCGGTCATCTCAATGGTGATATTGTGGTCGCGGAAGAAACGCTGCATATAGCCGGGATACTGGCCAc               <  2:23421/91‑1 (MQ=255)                                                              tGTTTTAAATCTTCTGCGTCACTTTCGGTCATCTCAATGGTGATATTGTGGTCGCGGAAGAAACGCTGCATATAGCCGGGATACTGGCCAc               <  2:712650/91‑1 (MQ=255)                                                              tGTTTTAAATCTTCTGCGTCACTTTCGGTCATCTCAATGGTGATATTGTGGTCGCGGAAGAAACGCTGCATATAGCCGGGATACTGGCCAc               <  1:422668/91‑1 (MQ=255)                                                              tGTTTTAAATCTTCTGCGTCACTTTCGGTCATCTCAATGGTGATATTGTGGTCGCGGAAGAAACGCTGCATATAGCCGGGATACTGGCCAc               >  2:809841/1‑91 (MQ=255)                                                                   tAAATCTTCTGCGTCACTTTCGGTCATCTCAATGGTGATATTGTGGTCGCGGAAGAAACGCTGCATATAGCCGGGATACTGGCCACGCGcc          <  1:697523/91‑1 (MQ=255)                                                                    aaaTCTTCTGCGTCACTTTCGGTCATCTCAATGGTGATATTGTGGTCGCGGAAGAAACGCTGCATATAGCCGGGATACTGGCCACGCGCCt         <  2:114500/91‑1 (MQ=255)                                                                     aaTCTTCTGCGTCACTTTCGGTCATCTCAATGGTGATATTGTGGTCGCGGAAGAAACGCTGCATATAGCCGGGATACTGGCCACGCGCCTg        >  1:78931/1‑91 (MQ=255)                                                                        cttctGCGTCACTTTCGGTCATCTCAATGGTGATATTGTGGTCGCGGAAGAAACGCTGCATATAGCCGGGATACTGGCCACGCGCCTGAAc     <  2:34008/91‑1 (MQ=255)                                                                         ttctGCGTCACTTTCGGTCATCTCAATGGTGATATTGTGGTCGCGGACGAAACGCTGCATATAGCCGGGATACTGGCCACGCGCTTGAACa    >  2:583826/1‑91 (MQ=255)                                                                           ctGCGTCACTTTCGGTCATCTCAATGGTGATATTGTGGTCGCGGAAGAAACGCTGCATATAGCCGGGATACTGGCCACGCGCCTGAACATc  <  1:222182/91‑1 (MQ=255)                                                                            tGCGTCACTTTCGGTCATCTCAATGGTGATATTGTGGTCGCGGAAGAAACGCTGCATATAGCCgg                           <  2:587523/65‑1 (MQ=255)                                                                            tGCGTCACTTTCGGTCATCTCAATGGTGATATTGTGGTCGCGGAAGAAACGCTGCATATAGCCgg                           >  1:587523/1‑65 (MQ=255)                                                                                        |                                                                              GCTTTCGTCGTGGGAAACACAACCAGTCATGTAATAACTAAAAGAGATGAAATCGACGGTATGTTTTAAATCTTCTGCGTCACTTTCAGTCATCTCAATGGTGATATTGTGGTCGCGGAAGAAACGCTGCATATAGCCGGGATACTGGCCACGCGCCTGAACATC  >  NZ_CP009273/3896132‑3896296

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑