Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,965,761	A→G	D381G (GAC→GGC)	wecC →	UDP‑N‑acetyl‑D‑mannosamine dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,965,761	0	A	G	100.0%	90.1 / NA	25	D381G (GAC→GGC)	wecC	UDP‑N‑acetyl‑D‑mannosamine dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (12/13);  total (12/13)

GGCGAAACTCTGGTTGTTGAGCCTAACATCCACCAGTTGCCGAAAAAACTGACCGGGCTTTGTACTCTGGCGCAGCTTGACGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCAGTTCAAAGTTATCAATGGCGACAATGTCCATCAGC  >  NZ_CP009273/3965682‑3965847                                                                                |                                                                                       ggcgAAACTCTGGTTGTTGAGCCTAACATCCACCAGTTGCCGAAAAAACTGACCGGGCTTTGTACTCTGGCGCAGCTTGGCGAGGCGCTgg                                                                             >  2:636006/1‑91 (MQ=255) ggcgAAACTCTGGTTGTTGAGCCTAACATCCACCAGTTGCCGAAAAAACTGACCGGGCTTTGTACTCTGGCGCAGCTTGGCGAGGCGCTgg                                                                             >  1:841150/1‑91 (MQ=255)            ggTTGTTGAGCCTAACATCCACCAGTTGCCGAAAAAACTGACCGGGCTTTGTACTCTGGCGCAGCTTGGCGAGGCGCTGGCAACGGCAGAc                                                                  <  2:63369/91‑1 (MQ=255)                gttgAGCCTAACATCCACCAGTTGCCGAAAAAACTGACCGGGCTTTGTACTCTGGCGCAGCTTGGCGAGGCGCTGGCAACGGCAGACGTGc                                                              <  2:562679/91‑1 (MQ=255)                      ccTAACATCCACCAGTTGCCGAAAAAACTGACCGGGCTTTGTACTCTGGCGCAGCTTGGCGAGGCGCTGGCAACGGCAGACGTGCTGGTGa                                                        >  1:568323/1‑91 (MQ=255)                         aaCATCCACCAGTTGCCGAAAAAACTGACCGGGCTTTGTACTCTGGCGCAGCTTGGCGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGc                                                     <  1:779597/91‑1 (MQ=255)                          aCATCCACCAGTTGCCGAAAAAACTGACCGGGCTTTGTACTCTGGCGCAGCTTGGCGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCt                                                    >  1:620750/1‑91 (MQ=255)                              ccagcaGTTGCCGAAAAATCTGACCGGGCGTTGTGCTCTGGCGCGACTTGGCGAGGCGGTGGCAGCGGCAGACGTGGTGGTGATGCTGGTc                                                <  2:830709/91‑1 (MQ=255)                              ccaccaGTTGCCGAAAAAACTGACCGGGCTTTGTGCTCTGGCGCAGCTTGGCGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTc                                                <  1:335093/91‑1 (MQ=255)                              ccaccaGTTGCCGAAAAAACTGACCGGGCTTTGTACTCTGGCGCAGCTTGGCGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTc                                                <  2:599750/91‑1 (MQ=255)                              ccaccaGTTGCCGAAAAAACTGACCGGGCTTTGTACTCTGGCGCAGCTTGGCGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTc                                                <  2:156500/91‑1 (MQ=255)                              ccaccaGTTGCCGAAAAAACTGACCGGGCTTTGTACTCTGGCGCAGCTTGGCGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTc                                                <  2:126459/91‑1 (MQ=255)                              ccaccaGTTGCCGAAAAAACTGACCGGGCTTTGTACTCTGGCGCAGCTTGGCGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTc                                                <  2:735792/91‑1 (MQ=255)                               caccaGTTGCCGAAAAAACTGACCGGGCTTTGTACTCTGGCGCAGCTTGGCGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCg                                               >  1:666948/1‑91 (MQ=255)                                    gTTGCCGAAAAAACTGACCGGGCTTTGTACTCTGGCGCAGCTTGGCGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCAt                                          >  1:772949/1‑91 (MQ=255)                                    gTTGCCGAAAAAACTGACCGGGCTTTGTACTCTGGCGCAGCTTGGCGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCAt                                          >  1:596313/1‑91 (MQ=255)                                      tGCCGAAAAAACTGACCGGGCTTTGTACTCTGGCGCAGCTTGGCGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAg                                        >  2:362213/1‑91 (MQ=255)                                                          cTTTGTACTCTGGCGCAGCTTGGCGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCAGTTCAAAGTTATCAATg                    <  1:636006/91‑1 (MQ=255)                                                                   ctGGCGCAGCTTGGCGAGGCGCTGGCAACGGCAGa                                                                   >  1:195033/1‑35 (MQ=255)                                                                   ctGGCGCAGCTTGGCGAGGCGCTGGCAACGGCAGa                                                                   <  2:195033/35‑1 (MQ=37)                                                                   ctGGCGCAGCTTGGCGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCAGTTCAAAGTTATCAATGGCGACAATg           >  1:8976/1‑91 (MQ=255)                                                                    tGGCGCAGCTTGGCGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCAGTTCAAAGTTATCAATGGCGACAATGt          <  1:443910/91‑1 (MQ=255)                                                                        gcAGCTTGGCGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCAGTTCAAAGTTATCAATGGCGACaa             >  1:734746/1‑84 (MQ=255)                                                                        gcAGCTTGGCGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCAGTTCAAAGTTATCAATGGCGACaa             <  2:734746/84‑1 (MQ=255)                                                                          aGCTTGGCGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCAGTTCAAAGTTATCAATGGCGACAATGTCCATca    >  1:331980/1‑91 (MQ=255)                                                                            cTTGGCGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCAGTTCAAAGTTATCAATGGCGACAATGTCCATcagc  <  2:441935/91‑1 (MQ=255)                                                                                |                                                                                       GGCGAAACTCTGGTTGTTGAGCCTAACATCCACCAGTTGCCGAAAAAACTGACCGGGCTTTGTACTCTGGCGCAGCTTGACGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCAGTTCAAAGTTATCAATGGCGACAATGTCCATCAGC  >  NZ_CP009273/3965682‑3965847

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑