Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	4,003,978	A→G	E140G (GAG→GGG)	yigL →	sugar/pyridoxal phosphate phosphatase YigL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	4,003,978	0	A	G	100.0%	89.9 / NA	25	E140G (GAG→GGG)	yigL	sugar/pyridoxal phosphate phosphatase YigL
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (16/9);  total (16/9)

TCGCCATCGCCCGGAAGAGATGCGCTTTTTTAAAGAAGCGGTGTTCCAATATGCGCTGTATGAGCCTGGATTACTGGAGCCGGAAGGCGTCAGCAAAGTGTTCTTCACCTGCGATTCCCATGAACAACTGCTGCCGCTGGAGCAGGCGATTAACGCTC  >  NZ_CP009273/4003901‑4004058                                                                              |                                                                                 tcgccatcgccCGGAAGAGATGCGCTTTTTTAAAGAAGCGGTGTTCCAATATGCGCTGTATGAGCCTGGATTACTGAGGCCGGAAGGCGTc                                                                     >  2:375439/1‑91 (MQ=255)      atcgccCGGAAGAGATGCGCTTTTTTAAAGAAGCGCTGTTCCAATATGCGCTGTATGAGCCTGGATTACTGGGGCCGGAAGGCGTCAGCaa                                                                <  2:290924/91‑1 (MQ=255)        cgccCGGAAGAGATGCGCTTTTTTAAAGAAGCGGTGTTCCAATATGCGCTGTATGAGCCTGGATTACTGGGGCCGGAAGGCGTCAGCAAAg                                                              <  2:477369/91‑1 (MQ=255)                    aTGCGCTTTTTTAAAGAAGCGGTGTTCCAATATGCGCTGTATGAGCCTGGATTACTGGGGCCGGAAGGCGTCAGCAAAGTGTTCTTCACCt                                                  >  2:124404/1‑91 (MQ=255)                    aTGCGCTTTTTTAAAGAAGCGGTGTTCCAATATGCGCTGTATGAGCCTGGATTACTGGGGCCGGAAGGCGTCAGCAAAGTGTTCTTCACCt                                                  >  1:853723/1‑91 (MQ=255)                        gcTTTTTTAAAGAAGCGGTGTTCCAATATGCGCTGTATGAGCCTGGATTACTGGGGCCGGAAGGCGTCAGCAAAGTGTTCTTCACCTGCGa                                              >  2:504991/1‑91 (MQ=255)                         cTTTTTTAAAGAAGCGGTGTTCCAATATGCGCTGTATGAGCCTGGATTACTGGGGCCGGAAGGCGTCAGCAAAGTGTTCTTCACCTGCGAt                                             <  2:437029/91‑1 (MQ=255)                           tttttAAAGAAGCGGTGTTCCAATATGCGCTGTATGAGCCTGGATTACTGGGGCCGGAAGGCGTCAGCAAAGTGTTCTTCACCTGCGATTc                                           >  2:343784/1‑91 (MQ=255)                            ttttAAAGAAGCGGTGTTCCAATATGCGCTGTATGAGCCTGGATTACTGGGGCCGGAAGGCGTCAGCAAAGTGTTCTTCCCCTGCGATTcc                                          <  2:41507/91‑1 (MQ=255)                                     agCGGTGTTCCAATATGCGCTGTATGAGCCTGGATTACTGGGGCCGGAAGGCGt                                                                      >  2:62909/1‑54 (MQ=255)                                     agCGGTGTTCCAATATGCGCTGTATGAGCCTGGATTACTGGGGCCGGAAGGCGt                                                                      <  1:62909/54‑1 (MQ=255)                                     agCGGTGTTCCAATATGCGCTGTATGAGCCTGGATTACTGGGGCCGGAAGGCGTCAGCAAAGTGTTCTTCACCTGCGATTGCCATGaacac                                 >  1:122558/1‑90 (MQ=255)                                          tgtTCCAATATGCGCTGTATGAGCCTGGATTACTGGGGCCGGAAGGCGTCAGCAACGTGTTCTTCACCTGCGATTCCCATGAACAActgct                            <  1:536673/91‑1 (MQ=255)                                           gtTCCAATATGCGCTGTATGAGCCTGGATTACTGGGGCCGGAAGGCGTCAGCAAAGTGTTCTTCACCTGCGATTCCCATGAACAActgctg                           <  1:466067/91‑1 (MQ=255)                                           gtTCCAATATGCGCTGTATGAGCCTGGATTACTGGGGCCGGAAGGCGTCAGCAAAGTGTTCTTCACCTGCGATTCCCATGAACAActgctg                           <  1:350737/91‑1 (MQ=255)                                                 atatGCGCTGTATGAGCCTGAATTACTGGGGCCGGAAGGCGTCAGc                                                                  <  1:573769/46‑1 (MQ=38)                                                 atatGCGCTGTATGAGCCTGAATTACTGGGGCCGGAAGGCGTCAGc                                                                  >  2:573769/1‑46 (MQ=38)                                                   atGCGCTGTATGAGCCTGGATTACTGGGGCCGGAAGGCGTCAGCAAAGTGTTCTTCACCTGCGATTCCCATGAACAACTGCTGCCGCTGGa                   >  1:785795/1‑91 (MQ=255)                                                     gcgcTGTATGAGCCTGGATTACTGGGGCCGGAAGGCGTCAGCAAAGTGTTCTTCACCTGCGATTCCCATGAACAACTGCTGCCGCTGGAGc                 >  1:90306/1‑91 (MQ=255)                                                     gcgcTGTATGAGCCTGGATTACTGGGGCCGGAAGGCGTCAGCAAAGTGTTCTTCACCTGCGATTCCCATGAACAACTGCTGCCGCTGGAGc                 >  2:32459/1‑91 (MQ=255)                                                          gTATGAGCCTGGATTACTGGGGCCGGAAGGCGTCAGCAAAGTGTTCTTCACCTGCGATTCCCATGAACAACTGCTGCCGCTGGAGCAGGCg            >  1:825647/1‑91 (MQ=255)                                                          gTATGAGCCTGGATTACTGGGGCCGGAAGGCGTCAGCAAAGTGTTCTTCACCTGCGATTCCCATGAACAACTGCTGCCGCTGGAGCAGGCg            >  1:397228/1‑91 (MQ=255)                                                                  cTGGATTACTGGGGCCGGAAGGCGTCAGCAAAGTGTTCTTCACCTGCGATTCCCATGAACAACTGCTGCCGCTGGAGCAGGCGATTAACGc    >  1:758314/1‑91 (MQ=255)                                                                  cTGGATTACTGGGGCCGGAAGGCGTCAGCAAAGTGTTCTTCACCTGCGATTCCCATGAACAACTGCTGCCGCTGGAGCAGGCGATTAACGc    >  1:361246/1‑91 (MQ=255)                                                                    ggATTACTGGGGCCGGAAGGCGTCAGCAAAGTGTTCTTCACCTGCGATTCCCATGAACAACTGCTGCCGCTGGAGCAGGCGATTAACGCTc  >  1:231630/1‑91 (MQ=255)                                                                              |                                                                                 TCGCCATCGCCCGGAAGAGATGCGCTTTTTTAAAGAAGCGGTGTTCCAATATGCGCTGTATGAGCCTGGATTACTGGAGCCGGAAGGCGTCAGCAAAGTGTTCTTCACCTGCGATTCCCATGAACAACTGCTGCCGCTGGAGCAGGCGATTAACGCTC  >  NZ_CP009273/4003901‑4004058

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑