Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	4,052,334	A→G	I190V (ATT→GTT)	typA →	ribosome‑dependent GTPase TypA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	4,052,334	0	A	G	100.0%	92.3 / NA	26	I190V (ATT→GTT)	typA	ribosome‑dependent GTPase TypA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (17/9);  total (17/9)

TCTGCGCTGAACGGTATCGCGGGTCTGGACCACGAAGATATGGCGGAAGACATGACCCCGCTGTACCAGGCGATTGTTGACCACGTTCCTGCGCCGGACGTTGACCTTGACGGTCCGTTCCAGATGCAGATTTCTCAGCTCGATTACAACAGCTATGT  >  NZ_CP009273/4052262‑4052419                                                                         |                                                                                      tCTGCGCTGAACGGTATCGCGGGTCTGGACCACGAAGATATGGCGGAAGACATGACCCCGCTGTACCAGGCGGTTGTTGACCACGTTCCTg                                                                     >  1:338703/1‑91 (MQ=255) tCTGCGCTGAACGGTATCGCGGGTCTGGACCACGAAGATATGGCGGAAGACATGACCCCGCTGTACCAGGCGGTTGTTGACCACGTTCCTg                                                                     >  1:398950/1‑91 (MQ=255)         gAACGGTATCGCGGGTCTGGACCACGAAGATATGGCGGAAGACATGACCCCGCTGTACCAGGCGGTTGTTGACCACGTTCCTGCCCCGGAc                                                             >  2:808199/1‑91 (MQ=255)           aCGGTATCGCGGGTCTGGACCACGAAGATATGGCGGAAGACATGACCCCGCTGTACCAGGCGGTTGTTGACCACGTTCCTGCGCCGGACGt                                                           <  1:9235/91‑1 (MQ=255)                aTCGCGGGTCTGGACCACGAAGATATGGCGGAAGACATGACCCCGCTGTACCAGGCGGTTGTTGACCACGTTCCTGCGCCGGACGTTGAcc                                                      <  2:654903/91‑1 (MQ=255)                 tCGCGGGTCTGGACCACGAAGATATGGCGGAAGACATGACCCCGCTGTACCAGGCGGTTGTTGACCACGTTCCTGCGCCGGACGTTGACCt                                                     >  1:476022/1‑91 (MQ=255)                        tCTGGACCACGAAGATATGGCGGAAGACATGACCCCGCTGTACCAGGCGGTTGTTGACGACGTTCCTGCGCCGGACGTTGACCTTGACGGt                                              >  2:434781/1‑91 (MQ=255)                        tCTGGACCACGAAGATATGGCGGAAGACATGACCCCGCTGTACCAGGCGGTTGTTGACCACGTTCCTGCGCCGGACGTTGACCTTGACGGt                                              >  1:852295/1‑91 (MQ=255)                         cTGGACCACGAAGATATGGCGGAAGACATGACCCCGCTGTACCAGGCGGTTGTTGACCACGTTCCTGCGCCGGACGTTGACCTTGACGGTc                                             <  1:639753/91‑1 (MQ=255)                          tGGACCACGAAGATATGGCGGAAGACATGACCCCGCTGTACCAGGCGGTTGTTGACCACGTTCCTGCGCCGGACGTTGACCTTGACGGTcc                                            <  2:855336/91‑1 (MQ=255)                                aCGAAGATATGGCGGAAGACATGACCCCGCTGTACCAGGCGGTTGTTGACCACGTTCCTGCGCCGGACGTTGACCTTGACGGTCCGTTCCa                                      >  2:68407/1‑91 (MQ=255)                                         tGGCGGAAGACATGACCCCGCTGTACCAGGCGGTTGTTGACCACGTTCCTGCGCCGGACGTTGACCTTGACGGTCCGTTCCAGATGCAGAt                             >  2:793906/1‑91 (MQ=255)                                             ggAAGACATGACCCCGCTGTACCAGGCGGTTGTTGACCACGTTCCTGCGCCGGACGTTGACCTTGACGGTCCGTTCCAGATGCAGATTtct                         <  2:820443/91‑1 (MQ=255)                                               aaGACATGACCCCGCTGTACCAGGCGGTTGTTGACCACGTTCCTGCGCCGGACGTTGACCTTGACGGTCCGTTCCAGATGCAGATTTCTCa                       <  1:93205/91‑1 (MQ=255)                                               aaGACATGACCCCGCTGTACCAGGCGGTTGTTGACCACGTTCCTGCGCCGGACGTTGACCTTGACGGTCCGTTCCAGATGCAGATTTCTCa                       >  2:112376/1‑91 (MQ=255)                                                  acacGACCCCGCTGTACCAGGCGGTTGTTGACCACGTTCCTGCGCCGGACGTTGACCTTGACGGTCCGTTCCAGATGCAGATTTCTCAGCt                    <  1:58559/91‑1 (MQ=255)                                                  aCATGACCCCGCTGTACCAGGCGGTTGTTGACCACGTTCCTGCGCCGGACGTTGACCTTGACGGTCCGTTCCAGATGCAGATTTCTCAGCt                    <  1:112376/91‑1 (MQ=255)                                                     tGACCCCGCTGTACCAGGCGGTTGTTGACCACGTTCCTGCGCCGGACGTTGACCTTGACGGTCCGTTCCAGATGCAGATTTCTCAGCTCGa                 >  1:73756/1‑91 (MQ=255)                                                     tGACCCCGCTGTACCAGGCGGTTGTTGACCACGTTCCTGCGCCGGACGTTGACCTTGACGGTCCGTTCCAGATGCAGATTTCTCAGCTCGa                 >  1:394604/1‑91 (MQ=255)                                                          ccGCTGTACCAGGCGGTTGTTGACCACGTTCCTGCGCCGGACGTTGACCTTGACGGTCCGTTCCAGATGCAGATTTCTCAGCTCGATTaca            >  1:412951/1‑91 (MQ=255)                                                          ccGCTGTACCAGGCGGTTGTTGACCACGTTCCTGCGCCGGACGTTGACCTTGACGGTCCGTTCCAGATGCAGATTTCTCAGCTCGATTaca            <  1:329536/91‑1 (MQ=255)                                                           cGCTGTACCAGGCGGTTGTTGACCACGTTCCTGCGCCGGACGTTGACCTTGACGGTCCGTTCCAGATGCAGATTTCTCAGCTCGATTacaa           >  2:421627/1‑91 (MQ=255)                                                            gCTGTACCAGGCGGTTGTTGACCACGTTCCTGCGCCGGACGTTGACCTTGACGGTCCGTTCCAGATGCAGATTTCTCAGCTCGATTacaac          >  1:622739/1‑91 (MQ=255)                                                            gCTGTACCAGGCGGTTGTTGACCACGTTCCTGCGCCGGACGTTGACCTTGACGGTCCGTTCCAGATGCAGATTTCTCAGCTCGATTacaac          >  2:581339/1‑91 (MQ=255)                                                                    aGGCGGTTGTTGACCACGTTCCTGCGCCGGACGTTGACCTTGACGGTCCGTTCCAGATGCAGATTTCTCAGCTCGATTACAACAGCTATGt  >  1:269092/1‑91 (MQ=255)                                                                    aGGCGGTTGTTGACCACGTTCCTGCGCCGGACGTTGACCTTGACGGTCCGTTCCAGATGCAGATTTCTCAGCTCGATTACAACAGCTATGt  >  1:248690/1‑91 (MQ=255)                                                                         |                                                                                      TCTGCGCTGAACGGTATCGCGGGTCTGGACCACGAAGATATGGCGGAAGACATGACCCCGCTGTACCAGGCGATTGTTGACCACGTTCCTGCGCCGGACGTTGACCTTGACGGTCCGTTCCAGATGCAGATTTCTCAGCTCGATTACAACAGCTATGT  >  NZ_CP009273/4052262‑4052419

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑