Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	4,132,297	T→C	intergenic (‑148/‑161)	ptsP ← / → frwC	phosphoenolpyruvate‑‑protein phosphotransferase/PTS fructose transporter subunit EIIC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	4,132,297	0	T	C	100.0%	91.6 / NA	25	intergenic (‑148/‑161)	ptsP/frwC	phosphoenolpyruvate‑‑protein phosphotransferase/PTS fructose transporter subunit EIIC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (13/12);  total (13/12)

CATGTGACAAATCTGCCAAAAGCTGGACAAATGTAATGTAACCGTCAATTTGCGACGCGTCTCACAAGACGCTGTTTTGCGGCATGCTTCCGGTTTATCGCAAGTTATGAGGCGGATCGCATTTTTGTACTGATATTACAAAAGTCCAGTAAATGGCCTTTTTATCCACTGTTT  >  NZ_CP009273/4132209‑4132382                                                                                         |                                                                                      cATGTGACAAATCTGCCAAAAGCTGGACAAATGTAATGTAACCGTCAATTTGCGACGCGTCTCACAAGACGCTGTTTTGCGGCATGCTccc                                                                                     <  2:811360/91‑1 (MQ=255)    gtgACAAATCTGCCAAAAGCTGGACAAATGTAATGTAACCGTCAATTTGCGACGCGTCTCACAAGACGCTGTTTTGCGGCATGCTCCCGGt                                                                                  >  1:778465/1‑91 (MQ=255)         aaaTCTGCCAAAAGCTGGACAAATGTAATGTAACCGTCAATTTGCGACGCGTCTCACAAGACGCTGTTTTGCGGCATGCTCCCGGTTTATc                                                                             >  2:465484/1‑91 (MQ=255)            tCTGCCAAAAGCTGGACAAATGTAATGTAACCGTCAATTTGCGACGCGTCTCACAAGACGCTGTTTTGCGGCATGCTCCCGGTTTATCGCa                                                                          <  2:50329/91‑1 (MQ=255)            tCTGCCAAAAGCTGGACAAATGTAATGTAACCGTCAATTTGCGACGCGTCTCACAAGACGCTGTTTTGCGGCATGCTCCCGGTTTATCGCa                                                                          <  1:381703/91‑1 (MQ=255)                       cTGGACAAATGTAATGTAACCGTCAATTTGCGACGCGTCTCACAAGACGCTGTTTTGCGGCATGCTCCCGGTTTATCGCAAGTTATGAGGc                                                               <  1:330544/91‑1 (MQ=255)                           aCAAATGTAATGTAACCGTCAATTTGCGACGCGTCTCACAAGACGCTGTTTTGCGGCATGCTCCCGGTTTATCGCAAGTTATGAGGCGGAt                                                           <  1:69031/91‑1 (MQ=255)                              aatgtaatgtAACCGTCAATTTGCGACGCGTCTCACAAGACGCTGTTTTGCGGCATGCTCCCGGTTTATCGCAAGTTATGAGGCGGATCGc                                                        <  1:513840/91‑1 (MQ=255)                              aatgtaatgtAACCGTCAATTTGCGACGCGTCTCACAAGACGCTGTTTTGCGGCATGCTCCCGGTTTATCGCAAGTTATGAGGCGGATCGc                                                        <  2:195367/91‑1 (MQ=255)                              aatgtaatgcAACCGTCAATTTGCGACGCGTCTCACAAGACGCTGTTTTGCGGCATGCTCCCGGTTTATCGCAAGTTATGAGGCGGATCGc                                                        <  2:258945/91‑1 (MQ=255)                                      gtaaCCGTCAATTTGCGACGCGTCTCACAAGACGCTGTTTTGCGGCATGCTCCCGGTTTATCGCAAGTTATGAGGCGGATCGCATTTTTGt                                                >  1:568773/1‑91 (MQ=255)                                        aaCCGTCAATTTGCGACGCGTCTCACAAGACGCTGTTTTGCGGCATGCTCCCGGTTTATCGCAAGTTATGAGGCGGATCGCATTTTTGTAc                                              >  2:747904/1‑91 (MQ=255)                                         aCCGTCAATTTGCGACGCGTCTCACAAGACGCTGTTTTGCGGCATGCTCCCGGTTTATCGCCAGTTATGAGGCGGATCGCATTTTTGTACt                                             >  2:685981/1‑91 (MQ=255)                                          ccGTCAATTTGCGACGCGTCTCACAAGACGCTGTTTTGCGGCATGCTCCCGGTTTATCGCAAGTTATGAGGCGGATCGCATTTTTGTACTg                                            <  1:325835/91‑1 (MQ=255)                                            gTCAATTTGCGACGCGTCTCACAAGACGCTGTTTTGCGGCATGCTCCCGGTTTATCGCAAGTTATGAGGCGGATCGCATTTTTGTACTGat                                          >  1:34172/1‑91 (MQ=255)                                                    gCGACGCGTCTCACAAGACGCTGTTTTGCGGCATGCTCCCGGTTTATCGCAAGTTATGAGGCGGATCGCATTTTTGTACTGATATTACaaa                                  >  1:401748/1‑91 (MQ=255)                                                    gCGACGCGTCTCACAAGACGCTGTTTTGCGGCATGCTCCCGGTTTATCGCAAGTTATGAGGCGGATCGCATTTTTGTACTGATATTACaaa                                  <  2:194770/91‑1 (MQ=255)                                                    gCGACGCGTCTCACAAGACGCTGTTTTGCGGCATGCTCCCGGTTTATCGCAAGTTATGAGGCGGATCGCATTTTTGTACTGATATTACaaa                                  <  1:378301/91‑1 (MQ=255)                                                    gCGACGCGTCTCACAAGACGCTGTTTTGCGGCATGCTCCCGGTTTATCGCAAGTTATGAGGCGGATCGCATTTTTGTACTGATATTACaaa                                  <  1:763030/91‑1 (MQ=255)                                                                      cGCTGTTTTGCGGCATGCTCCCGGTTTATCGCAAGTTATGAGGCGGATCGCATTTTTGTACTGATATTACAAAAGTCCAGTAAATGGCCtt                >  1:276261/1‑91 (MQ=255)                                                                      cGCTGTTTTGCGGCATGCTCCCGGTTTATCGCAAGTTATGAGGCGGATCGCATTTTTGTACTGATATTACAAAAGTCCAGTAAATGGCCtt                >  1:576123/1‑91 (MQ=255)                                                                        cTGTTTTGCGGCATGCTCCCGGTTTATCGCAAGTTATGAGGCGGATCGCATTTTTGTACTGATATTACAAAAGTCCAGTAAATGGCCtttt              >  1:483644/1‑91 (MQ=255)                                                                        cTGTTTTGCGGCATGCTCCCGGTTTATCGCAAGTTATGAGGCGGATCGCATTTTTGTACTGATATTACAAAAGTCCAGTAAATGGCCtttt              >  2:70684/1‑91 (MQ=255)                                                                              tGCGGCATGCTCCCGGTTTATCGCAAGTTATGAGGCGGATCGCATTTTTGTACTGATATTACAAAAGTCCAGTAAATGGCCTTTTTATCCa        <  1:443963/91‑1 (MQ=255)                                                                                    aTGCTCCCGGTTTATCGCAAGTTATGAGGCGGATCGCATTTTTGTACTGATATTACAAAAGTCCAGTAAATGGCCTTTTTATCCACTGttt  >  2:182906/1‑91 (MQ=255)                                                                                    aTGCTCCCGGTTTATCGCAAGTTATGAGGCGGATCGCATTTTTGTACTGATATTACAAAAGTCCAGTAAATGGCCTTTTTATCCACTGatt  >  1:269031/1‑91 (MQ=255)                                                                                         |                                                                                      CATGTGACAAATCTGCCAAAAGCTGGACAAATGTAATGTAACCGTCAATTTGCGACGCGTCTCACAAGACGCTGTTTTGCGGCATGCTTCCGGTTTATCGCAAGTTATGAGGCGGATCGCATTTTTGTACTGATATTACAAAAGTCCAGTAAATGGCCTTTTTATCCACTGTTT  >  NZ_CP009273/4132209‑4132382

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑