Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	4,172,602	A→C	E477A (GAG→GCG)	rpoB →	DNA‑directed RNA polymerase subunit beta

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	4,172,602	0	A	C	100.0%	89.6 / NA	25	E477A (GAG→GCG)	rpoB	DNA‑directed RNA polymerase subunit beta
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (16/9);  total (16/9)

CGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAG  >  NZ_CP009273/4172523‑4172682                                                                                |                                                                                 cgtcgtATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGCGCGTCTGtctc                                                                       >  1:66820/1‑91 (MQ=255) cgtcgtATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGCGCGTCTGtctc                                                                       >  2:517270/1‑91 (MQ=255)         ccgttccgttGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGCGCGTCTGTCTCTGGGCGAt                                                               <  2:681703/91‑1 (MQ=255)              ccgttGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGCGCGTCTGTCTCTGGGCGATCTGGa                                                          <  2:66820/91‑1 (MQ=255)                  tGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGCGCGTCTGTCTCTGGGCGATCTGGATAcc                                                      <  2:92655/91‑1 (MQ=255)                  tGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGCGCGTCTGTCTCTGGGCGATCTGGATAcc                                                      <  1:780538/91‑1 (MQ=255)                     cGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGCGCGTCTGTCTCTGGGCGATCTGGATACCCTg                                                   >  2:585713/1‑91 (MQ=255)                            gCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGCGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCcac                                            <  2:792272/91‑1 (MQ=255)                             cGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGCGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCcaca                                           <  1:327197/91‑1 (MQ=255)                             cGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGCGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCcaca                                           <  1:345683/91‑1 (MQ=255)                                  aaCCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGCGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGata                                      <  2:37844/91‑1 (MQ=255)                                       gTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGCGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATGATc                                 >  1:282334/1‑91 (MQ=255)                                                           gtgtAGAGCGTGCGGTGAAAGCGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATGATCAACGCCAAGCCGATTTCCgc             >  2:159645/1‑91 (MQ=255)                                                           gtgtAGAGCGTGCGGTGAAAGCGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATGATCAACGCCAAGCCGATTTCCgc             >  1:777574/1‑91 (MQ=255)                                                           gtgtAGAGCGTGCGGTGAAAGCGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATGATCAACGCCAAGCCGATTTCCgc             >  1:373761/1‑91 (MQ=255)                                                                gagCGTGCGGTGAAAGCGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATGATCAACGCCAAGCCGATTTCCGcagcag        >  2:634456/1‑91 (MQ=255)                                                                    gTGCGGTGAAAGCGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATGATCACCGCCAAGCCGATTTCCGCAGCAGTGaa    >  2:628176/1‑91 (MQ=255)                                                                    gTGCGGTGAAAGCGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGaa    >  1:391450/1‑91 (MQ=255)                                                                    gTGCGGTGAAAGCGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGaa    >  2:784056/1‑91 (MQ=255)                                                                     tGCGGTGAAAGCGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATGATCAACGCCAAGCCGATTTCCgcagc          <  1:692057/84‑1 (MQ=255)                                                                     tGCGGTGAAAGCGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATGATCAACGCCAAGCCGATTTCCgcagc          >  2:692057/1‑84 (MQ=255)                                                                     tGCGGTGAAAGCGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGaaa   >  2:280603/1‑91 (MQ=255)                                                                     tGCGGTGAAAGCGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGaaa   >  2:602146/1‑91 (MQ=255)                                                                      gCGGTGAAAGCGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAag  >  1:217770/1‑91 (MQ=255)                                                                      gCGGTGAAAGCGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAag  >  2:733602/1‑91 (MQ=255)                                                                                |                                                                                 CGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAG  >  NZ_CP009273/4172523‑4172682

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑