Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	4,223,121	A→G	V14A (GTA→GCA)	lysC ←	lysine‑sensitive aspartokinase 3

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	4,223,121	0	A	G	100.0%	81.3 / NA	22	V14A (GTA→GCA)	lysC	lysine‑sensitive aspartokinase 3
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (5/17);  total (5/17)

CAGCAGAAGCCGAGAGGACAACTAAACGCACGTTGGCATCAGAAAGCACAATATCAGCGCTGCGGTTCATGGCGTCAAAATCAGCTACGCTGGTACCGCCAAATTTGGAGACAACAATTTCAGACATAACTACCTCGTGTCAGGGGATCCATTTTCAGCCTTGGCACAAGGGA  >  NZ_CP009273/4223035‑4223207                                                                                       |                                                                                       cagcagAAGCCGAGAGGACAACTAAACGCACGTTGGCATCAGAAAGCACAATATCAGCGCTGCGGTTCATGGCGTCAAAATCAGCTGCGCt                                                                                    >  2:517757/1‑91 (MQ=255)        aGCCGAGAGGACAACTAAACGCACGTTGGCATCAGAAAGCACAATATCAGCGCTGCGGTTCATGGCGTCAAAATCAGCTGCGCTGGTACCg                                                                             >  1:83671/1‑91 (MQ=255)               agGACAACTAAACGCACGTTGGCATCAGAAAGCACAATATCAGCGCTGCGGTTCATGGCGTCAAAATCAGCTGCGCTGGTACCGCCAAAtt                                                                      <  2:42010/91‑1 (MQ=255)                    aaCTAAACGCACGTTGGCATCAGAAAGCACAATATCAGCGCTGCGGTTCATGGCGTCAAAATCAGCTGCGCTGGTACCGCCAAATTTGgag                                                                 <  1:730045/91‑1 (MQ=255)                         aaCGCACGTTGGCATCAGAAAGCACAATATCAGCGCTGCGGTCCATGGCGTCAAAATCAGCTGCGCTGGTACCGCCAAATTTGGAGacaac                                                            <  1:816218/91‑1 (MQ=255)                               cGTTGGCATCAGAAAGCACAATATCAGCGCTGCGGTTCATGGCGTCAAAATCAGCTGCGCTGGTACCGCCAAATTTGGAGACAACAATTTc                                                      <  2:870712/91‑1 (MQ=255)                                 ttGGCATCAGAAAGCACAATATCAGCGCTGCGGTTCATGGCGTCAAAATCAGCTGCGCTGGTACCGCCAAATTTGGAGACAACAATTTCAg                                                    <  2:831520/91‑1 (MQ=255)                                 ttGGCATCAGAAAGCACAATATCAGCGCTGCGGTTCATGGCGTCAAAATCAGCTGCGCTGGTACCGCCAAATTTGGAGACAACAATTTCAg                                                    <  1:388693/91‑1 (MQ=255)                                 ttGGCATCAGAAAGCACAATATCAGCGCTGCGGTTCATGGCGTCAAAATCAGCTGCGCTGGTACCGCCAAATTTGGAGACAACAATTTCAg                                                    <  2:234164/91‑1 (MQ=255)                                   ggCATCAGAAAGCACAATATCAGCGCTGCGGTTCATGGCGTCAAAATCAGCTGCGCTGGTACCGCCAAATTTGGAGACAACAATTTCAGAc                                                  <  1:198773/91‑1 (MQ=255)                                   ggCATCAGAAAGCACAATATCAGCGCTGCGGTTCATGGCGTCAAAATCAGCTGCGCTGGTACCGCCAAATTTGGAGACAACAATTTCAGAc                                                  <  2:641915/91‑1 (MQ=255)                                   ggCATCAGAAAGCACAATATCAGCGCTGCGGTTCATGGCGTCAAAATCAGCTGCGCTGGTACCGCCAAATTTGGAGACAACAATTTCAGAc                                                  <  1:528029/91‑1 (MQ=255)                                   ggCATCAGAAAGCACAATATCAGCCCTGCGGTTCATGGCGTCAAAATCAGCTGCGCTGGTACCGCCAAATTTGGAGACAACAATTTCAGAc                                                  <  2:841557/91‑1 (MQ=255)                                     cATCAGAAAGCACAATATCAGCGCTGCGGTTCATGGCGTCAAAATCAGCTGCGCTGGTACCGCCAAATTTGGAGACAACAATTTCAGACAt                                                <  2:666104/91‑1 (MQ=255)                                         aGAAAGCACAATATCAGCGCTGCGGTTCATGGCGTCAAAATCAGCTGCGCTGGTACCGCCAAATTTGGAGACAACAATTTCAGACATAACt                                            <  2:509330/91‑1 (MQ=255)                                                 caATATCAGCGCTGCGGTTCATGGCGTCAAAATCAGCTGCGCTGGTACCGCCAAATTTGGAGACAACAATTTCAGACATAACTACCTCgtg                                    >  1:68207/1‑91 (MQ=255)                                                        aGCGCTGCGGTTCATGGCGTCAAAATCAGCTGCGCTGGTACCGCCAAATTTGGAGACAACAATTTCAGACATAACTACCTCGTGTCAgggg                             >  1:534901/1‑91 (MQ=255)                                                            cTGCGGTTCATGGCGTCAAAATCAGCTGCGCTGGTACCGCCAAATTTGGAGACAACAATTTCAGACATAACTACCTCGTGTCAGGGGATcc                         <  1:517757/91‑1 (MQ=255)                                                                   tCATGGCGTCAAAATCAGCTGCGCTGGTACCGCCAAATTTGGAGACAACAATTTCAGACATAACTACCTCGTGTCAGGGGATCCATTTTCa                  <  2:358715/91‑1 (MQ=255)                                                                        gCGTCAAAATCAGCTGCGCTGGTACCGCCAAATTTGGAGACAACAATTTCAGACATAACTACCTCGTGTCAGGGGATCCATTTTCAGCCtt             <  1:180515/91‑1 (MQ=255)                                                                           tCAAAATCAGCTGCGCTGGTACCGCCAAATTTGGAGACAACAATTTCAGACATAACTACCTCGTGTCAGGGGATCCATTTTCAGCCTTGGc          >  2:211369/1‑91 (MQ=255)                                                                                   aGCTGCGCTGGTACCGCCAAATTTGGAGACAACAATTTCAGACATAACTACCTCGTGTCAGGGGATCCATTTTCAGCCTTGGCACAAGGGa  <  1:31071/91‑1 (MQ=255)                                                                                       |                                                                                       CAGCAGAAGCCGAGAGGACAACTAAACGCACGTTGGCATCAGAAAGCACAATATCAGCGCTGCGGTTCATGGCGTCAAAATCAGCTACGCTGGTACCGCCAAATTTGGAGACAACAATTTCAGACATAACTACCTCGTGTCAGGGGATCCATTTTCAGCCTTGGCACAAGGGA  >  NZ_CP009273/4223035‑4223207

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑