| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 4,235,276 | T→C | N358D (AAC→GAC) | malE ← | maltose/maltodextrin ABC transporter substrate‑binding protein MalE |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 4,235,276 | 0 | T | C | 100.0% | 54.4 / NA | 16 | N358D (AAC→GAC) | malE | maltose/maltodextrin ABC transporter substrate‑binding protein MalE |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (11/5); total (11/5) | |||||||||||
GCTTCATCGACAGTCTGACGACCGCTGGCGGCGTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTCCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTT > NZ_CP009273/4235191‑4235357 | gCTTCATCGACAGTCTGACGACCGCTGGCGGCGTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTCCGGCa < 1:856513/91‑1 (MQ=255)gCTTCATCGACAGTCTGACGACCGCTGGCGGCGTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTCCGGCa < 2:731286/91‑1 (MQ=255)gCTTCATCGACAGTCTGACGACCGCTGGCGGCGTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTCCGGCa < 2:867204/91‑1 (MQ=255) tCATCGACAGTCTGACGACCGCTGGCGGCGTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTCCGGCATGa > 2:274049/1‑91 (MQ=255) cATCGACAGTCTGACGACCGCTGGCGGCGTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTCCGGCATGAt < 1:85321/91‑1 (MQ=255) gTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTCCGGCATGATTTCACCTTTCTGGGCGTTTTCCATGGTGGCGGCAATACGTg > 1:431235/1‑91 (MQ=255) cACGGCATACCAGAAAGCGGACATCTGCGGGATGTCCGGCATGATTTCACCTTTCTGGGCGTTTTCCATGGTGGCGGCAATACGTGGATCt > 1:595559/1‑91 (MQ=255) cACGGCATACCAGAAAGCGGACATCTGCGGGATGTCCGGCATGATTTCACCTTTCTGGGCGTTTTCCATGGTGGCGGCAATACGTGGATCt > 1:657574/1‑91 (MQ=255) tACCAGAAAGCGGACATCTGCGGGATGTCCGGCATGATTTCACCTTTCTGGGCGTTTTCCATGGTGGCGGCAATACGTgg < 1:212714/80‑1 (MQ=255) tACCAGAAAGCGGACATCTGCGGGATGTCCGGCATGATTTCACCTTTCTGGGCGTTTTCCATGGTGGCGGCAATACGTgg > 2:212714/1‑80 (MQ=255) tACCAGAAAGCGGACATCTGCGGGATGTCCGGCATGATTTCACCTTTCTGGGCGTTTTCCATGGTGGCGGCAATACGTGGATCTTTCGCCa > 2:25969/1‑91 (MQ=255) aaGCGGACATCTGCGGGCTGTCCGGCATGATTTCACATTTCTGGGCGTTTTCCATGGAGGCGGCAATACGTGGATCTTTCGCCAACTCTTc > 2:404721/1‑91 (MQ=255) aaGCGGACATCTGCGGGATGTCCGGCATGATTTCACCTTTCTGGGCGTTTTCCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTc > 2:525145/1‑91 (MQ=255) aaGCGGACATCTGCGGGATGTCCGGCATGATTTCACCTTTCTGGGCGTTTTCCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTc > 2:658870/1‑91 (MQ=255) gccGGACATCTGCGGGATGTCCGGCATGATTTCACCTTTCTGGGCGTTTTCCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTcc > 2:182775/3‑91 (MQ=255) gCGGGGTGTCCGGCATGATTTCACCTTTCTGGGGGTTTTCCATGGTGGCGGCAATACGTGGATCTTTTGCCAACTCTTCCTCGTAAGACtt > 2:700672/1‑91 (MQ=255) | GCTTCATCGACAGTCTGACGACCGCTGGCGGCGTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTCCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTT > NZ_CP009273/4235191‑4235357 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |