| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 4,251,021 | A→G | pseudogene (773/986 nt) | BW25113_RS21020 → | DUF2713 domain‑containing protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 4,251,021 | 0 | A | G | 97.1% | 124.7 / NA | 34 | pseudogene (773/986 nt) | BW25113_RS21020 | DUF2713 domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (15/18); minor base T (0/1); total (15/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GAGCTACCTGAAAGCTCAGTTTGAAAAATTATCTCAACGTGATATGAAGAAGTACCTGGGAGAGATGAAGCGTCTGGCTGATGGGGGAGAAATGAATTTTGATGGCTATCTGGATAAGATGTCACATCTGGTGAATGAAGGAACGCTCGATCCTGATATTTTAAGCAAAATGCGA > NZ_CP009273/4250934‑4251108 | gagCTACCTGAAAGCTCAGTTTGAAAAATTATCTCAACGTGATATGAAGAAGTACCTGGGAGAGATGAAGCGTCTGGCTGATGGGGGGGaa > 1:85507/1‑91 (MQ=255) gCTCAGTTTGAAAAATTATCTCAACGTGATATGAAGAAGTACCTGGGAGAGATGAAGCGTCTGGCTGATGGGGGGGAAATGAATTTTGATg > 1:598229/1‑91 (MQ=255) tCAGTTTGAAAAATTATCTCAACGTGATATGAAGAAGTACCTGGGAGAGATGAAGCGTCTGGCTGATGGGGGGGAAATGAATTTTGATGGc > 2:403113/1‑91 (MQ=255) tCAGTTTGAAAAATTATCTCAACGTGATATGAAGAAGTACCTGGGAGAGATGAAGCGTCTGGCTGATGGGGGGGAAATGAATTTTGATGGc > 2:178909/1‑91 (MQ=255) tCAGTTTGAAAAATTATCTCAACGTGATATGAAGAAGTACCTGGGAGAGATGAAGCGTCTGGCTGATGGGGGGGAAATGAATTTTGATGGc < 1:448125/91‑1 (MQ=255) aGTTTGAAAAATTATCTCAACGTGATATGAAGAAGTACCTGGGAGAGATGAAGCGTCTGGCTGATGGGGGGGAAATGAATTTTGATGGCTa < 2:684375/91‑1 (MQ=255) tttGAAAAATTATCTCAACGTGATATGAAGAAGTACCTGGGAGAGATGAAGCGTCTGGCTGATGGGGGGGAAATGAATTTTGATGGCTATc < 2:2472/91‑1 (MQ=255) tttGAAAAATTATCTCAACGTGATATGAAGAAGTACCTGGGAGAGATGAAGCGTCTGGCTGATGGGGGGGAAATGAATTTTGATGGCTATc < 1:503133/91‑1 (MQ=255) tttGAAAAATTATCTCAACGTGATATGAAGAAGTACCTGGGAGAGATGAAGCGTCTGGCTGATGGGGGGGAAATGAATTTTGATGGCTATc < 1:788469/91‑1 (MQ=255) tttGAAAAATTATCTCAACGTGAGATGAAGAAGTACCTGGGAGAGATGAAGCGTCTGGCTGATGGGGGGGAAATGAATTTTGATGGCTATc < 1:233596/91‑1 (MQ=255) tttGAAAAAGTATCTCAACGTGATATGAAGAAGTACCTGGGAGAGATGAAGCGTCTGGCTGATGGGGGGGAAATGAATTTTGATGGCTATc < 1:684688/91‑1 (MQ=255) ttGAAAAATTATCTCAACGTGATATGAAGAAGTACCTGGGAGAGATGAAGCGTCTGGCTGATGGGGGGGAAATGAATTTTGATGGCTATCt > 2:640530/1‑91 (MQ=255) aaaaTTATCTCAACGTGATATGAAGAAGTACCTGGGAGAGATGAAGCGTCTGGCTGATGGGGGGGAAATGAATTTTGATGGCTATCTGGAt < 1:2250/91‑1 (MQ=255) aaTTATCTCAACGTGATATGAAGAAGTACCTGGGAGAGATGAAGCGTCTGGCTGATGGGGGGGAAATGAATTTTGATGGCTATCTGGATaa < 2:809736/91‑1 (MQ=255) aaCGTGATATGAAGAAGTACCTGGGAGAGATGAAGCGTCTGGCTGATGGGGGGGAAATGAATTTTGATGGCTATCTGGATAAGATGTcaca < 1:403113/91‑1 (MQ=255) tGATATGAAGAAGTACCTGGGAGAGATGAAGCGTCTGGCTGATGGGGGGGAAATGAATTTTGATGGCTATCTGGATAAGATGTCACATCTg < 1:412674/91‑1 (MQ=255) gATATGAAGAAGTACCTGGGAGAGATGAAGCGTCTGGCTGATGGGGGGGAAATGAATTTTGATGGCTATCTGGATAAGATGTCACATCTgg > 2:847110/1‑91 (MQ=255) gATATGAAGAAGTACCTGGGAGAGATGAAGCGTCTGGCTGATGGGGGGGAAATGAATTTTGATGGCTATCTGGATAAGATGTCACATCTgg < 2:290135/91‑1 (MQ=255) gATATGAAGAAGTACCTGGGAGAGATGAAGCGTCTGGCTGATGGGGGGGAAATGAATTTTGATGGCTATCTGGATAAGATGTCACATCTgg > 2:366247/1‑91 (MQ=255) tatGAAGAAGTACCTGGGAGAGATGAAGCGTCTGGCTGATGGGGGGGAAATGAATTTTGATGGCTATCTGGATAAGATGTCACATCTGGtg < 1:52567/91‑1 (MQ=255) agTACCTGGGAGAGATGAAGCGTCTGGCTGATGGGGGGGAAATGAATTTTGATGGCTATCTGGATAAGATGTCACATCTGGTGAATGAAgg > 1:418003/1‑91 (MQ=255) gTACCTGGGAGAGATGAAGCGTCTGGCTGATGGGGGGGAAATGAATTTTg < 1:852717/50‑1 (MQ=255) gTACCTGGGAGAGATGAAGCGTCTGGCTGATGGGGGGGAAATGAATTTTg > 2:852717/1‑50 (MQ=255) cTGGGAGAGATGAAGCGTCTGGCTGATGGGGGGGAAATGAATTTTGATGGCTATCTGGATAAGATGTCACATCTGGTGAATGAAGGAACGc > 2:707175/1‑91 (MQ=255) gggAGAGATGAAGCGTCTGGCTGATGGGGGTGAAATGAATTTTGATGGCTATCTGGATAAGATGTCACATCTGGTGAAGGAAGGAACGCTc < 2:273173/91‑1 (MQ=255) gggAGAGATGAAGCGTCTGGCTGATGGGGGGGAAATGAATTTTGATGGCTATCTGGATAAGATGTCACATCTGGTGAATGAAGGAACGCTc < 2:857678/91‑1 (MQ=255) gggAGAGATGAAGCGTCTGGCTGATGGGGGGGAAATGAATTTTGATGGCTATCTGGATAAGATGTCACATCTGGTGAATGAAGGAACGCTc < 2:418003/91‑1 (MQ=255) agagaTGAAGCGTCTGGCTGATGGGGGGGAAATGAATTTTGATGGCTATCTGGATAAGATGTCACATCTGGTGAATGAAGGAACGCTCGAt < 1:105693/91‑1 (MQ=255) tGAAGCGTCTGGCTGATGGGGGGGAAATGAATTTTGATGGCTATCTGGATAAGATGTCACATCTGGTGAATGAAGGAACGCTCGATCCTGa < 2:439924/91‑1 (MQ=255) gTCTGGCTGATGGGGGGGCAATGAATTTTGATGGCTATCTGGATCAGATGTCACATCTGGTGAATGAAGGAACGCTCGATCCTGATAtttt > 2:197914/1‑91 (MQ=255) gTCTGGCTGATGGGGGGGAAATGAATTTTGATGGCTATCTGGATAAGATGTCACATCTGGTGAATGAAGGAACGCTCGATCCTGATAtttt > 2:183/1‑91 (MQ=255) gTCTGGCTGATGGGGGGGAAATGAATTTTGATGGCTATCTGGATAAGATGTCACATCTGGTGAATGAAGGAACGCTCGATCCTGATAtttt > 2:133689/1‑91 (MQ=255) gTCTGGCTGATGGGGGGGAAATGAATTTTGATGGCTATCTGGATAAGATGTCACATCTGGTGAATGAAGGAACGCTCGATCCTGATAtttt > 1:112385/1‑91 (MQ=255) aTGGGGGGGAAATGAATTTTGATGGCTATCTGGATAAGATGTCACATCTGGTGAATGAAGGAACGCTCGATCCTGATATTTTAAGCAAAAt > 1:727065/1‑91 (MQ=255) ggggGAAATGAATTTTGATGGCTATCTGGATAAGATGTCACATCTGGTGAATGAAGGAACGCTCGATCCTGATATTTTAAGCAAAATGCga < 2:724601/91‑1 (MQ=255) | GAGCTACCTGAAAGCTCAGTTTGAAAAATTATCTCAACGTGATATGAAGAAGTACCTGGGAGAGATGAAGCGTCTGGCTGATGGGGGAGAAATGAATTTTGATGGCTATCTGGATAAGATGTCACATCTGGTGAATGAAGGAACGCTCGATCCTGATATTTTAAGCAAAATGCGA > NZ_CP009273/4250934‑4251108 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |