Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	4,264,031	A→T	intergenic (‑232/‑22)	uvrA ← / → ssb1	excinuclease ABC subunit UvrA/single‑stranded DNA‑binding protein SSB1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	4,264,031	0	A	T	100.0%	157.8 / NA	41	intergenic (‑232/‑22)	uvrA/ssb1	excinuclease ABC subunit UvrA/single‑stranded DNA‑binding protein SSB1
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (26/15);  total (26/15)

CAGGTTTGTTTCCCGGAACCGAGGTCACAACATAGTAAAAGCGCTATTGGTAATGGTACAATCGCGCGTTTACACTTATTCAGAACGATTTTTTTCAGGAGACACGAACATGGCCAGCAGAGGCGTAAACAAGGTTATTCTCGTTGGTAATCTGGGTCAGGACCCGGAAGTAC  >  NZ_CP009273/4263944‑4264116                                                                                        |                                                                                      cAGGTTTGTTTCCCGGAACCGAGGTCACAACATAGTAAAAGCGCTATTGGTAATGGTACAATCGCGCGTTTACACTTATTCAGAACGtttt                                                                                    <  2:128905/91‑1 (MQ=255)        gtttCCCGGAACCGAGGTCACAACATAGTAAAAGCGCTATTGGTAATGGTACAATCGCGCGTTTACACTTATTCAGAACGTTTTTTTTCAg                                                                             >  1:570461/1‑91 (MQ=255)           tCCCGGAACCGAGGTCACAACATAGTAAAAGCGCTATTGGTAATGGTACAATCGCGCGTTTACACTTATTCAGAACGTTTTTTTTCAGgag                                                                          <  2:116307/91‑1 (MQ=255)             ccGGAACCGAGGTCACAACATAGTAAAAGCGGTATTGGTAATGGTACAATCGCGCGTTTACACTTATTCAGAACGTTTTTTTTGAGGAGac                                                                        >  1:832256/1‑91 (MQ=255)             ccGGAACCGAGGTCACAACATAGTAAAAGCGCTATTGGTAATGGTACAATCGCGCGTTTACACTTATTCAGAACGTTTTTTTTCAGGAGac                                                                        >  1:388314/1‑91 (MQ=255)                  aCCGAGGTCACAACATAGTAAAAGCGCTATTGGTAATGGTACAATCGCGCGTTTACACTTATTCAGAACGTTTTTTTTCAGGAGACACGaa                                                                   >  1:841594/1‑91 (MQ=255)                    cGAGGTCACAACATAGTAAAAGCGCTATTGGTAATGGTACAATCGCGCGTTTACACTTATTCAGAACGTTTTTTTTCAGGAGACACGAACa                                                                 <  1:212089/91‑1 (MQ=255)                    cGAGGTCACAACATAGTAAAAGCGCTATTGGTAATGGTACAATCGCGCGTTTACACTTATTCAGAACGTTTTTTTTCAGGAGACACGAACa                                                                 <  2:108773/91‑1 (MQ=255)                     gAGGTCACAACATAGTAAAAGCGCTATTGGTAATGGTACAATCGCGCGTTTACACTTATTCAGAACGTTTTTTTTCAGGAGACACGAACAt                                                                >  2:31036/1‑91 (MQ=255)                              acaTAGTAAAAGCGCTATTGGTAATGGTACAATCGCGCGTTTACACTTATTCAGAACGTTTTTTTTCAGGAGACACGAACATGGCcagcag                                                       <  1:775174/91‑1 (MQ=255)                                  aGTAAAAGCGCTATTGGTAATGGTACAATCGCGCGTTTACACTTATTCAGAACGTTTTTTTTCAGGAGACACGAACATGGCCAGCAGAGGc                                                   <  2:117121/91‑1 (MQ=255)                                      aaaGCGCTATTGGTAATGGTACAATCGCGCGTTTACACTTATTCAGAACGTTTTTTTTCAGGAGACACGAACATGGCCAGCAGAGGCGTaa                                               >  1:530279/1‑91 (MQ=255)                                         gcgcTATTGGTAATGGTACAATCGCGCGTTTACACTTATTCAGAACGTTTTTTTTCAGGAGACACGAACATGGCCAGCAGAGGCGTAAACa                                            <  2:540573/91‑1 (MQ=255)                                           gcTATTGGTAATGGTACAATCGCGCGTTTACACTTATTCAGAACGTTTTTTTTCAGGAGACACGAACATGGCCAGCAGAGGCGTAAACAAg                                          >  1:192530/1‑91 (MQ=255)                                           gcTATTGGTAATGGTACAATCGCGCGTTTACACTTATTCAGAACGTTTTTTTTCAGGAGACACGAACATGGCCAGCAGAGGCGTAAACAAg                                          <  2:469711/91‑1 (MQ=255)                                             tATTGGTAATGGTACAATCGCGCGTTTACACTTATTCAGAACGTTTTTTTTCAGGAGaca                                                                       >  2:693216/1‑60 (MQ=255)                                             tATTGGTAATGGTACAATCGCGCGTTTACACTTATTCAGAACGTTTTTTTTCAGGAGaca                                                                       <  1:693216/60‑1 (MQ=255)                                             tATTGGTAATGGTACAATCGCGCGTTTACACTTATTCAGAACGTTTTTTTTCAGGAGACACGAACATGGCCAGCAGAGGCGTAAACAAGGt                                        >  1:631776/1‑91 (MQ=255)                                             tATTGGTAATGGTACAATCGCGCGTTTACACTTATTCAGAACGTTTTTTTTCAGGAGACACGAACATGGCCAGCAGAGGCGTAAACAAGGt                                        >  1:737973/1‑91 (MQ=255)                                             tATTGGTAATGGTACAATCGCGCGTTTACACTTATTCAGAACGTTTTTTTTCAGGAGACACGAACATGGCCAGCAGAGGCGTAAACAAGGt                                        >  2:607420/1‑91 (MQ=255)                                             tATTGGTAATGGTACAATCGCGCGTTTACACTTATTCAGAACGTTTTTTTTCAGGAGACACGAACATGGCCAGCAGAGGCGTAAACAAGGt                                        >  1:379459/1‑91 (MQ=255)                                             tATTGGTAATGGTACAATCGCGCGTTTACACTTATTCAGAACGTTTTTTTTCAGGAGACACGAACATGGCCAGCAGAGGCGTAAACAAGGt                                        >  1:342962/1‑91 (MQ=255)                                             tATTGGTAATGGTACAATCGCGCGTTTACACTTATTCAGAACGTTTTTTTTCAGGAGACACGAACATGGCCAGCAGAGGCGTAAACAAGGt                                        >  1:314108/1‑91 (MQ=255)                                                        gTACAATCGCGCGTTTACACTTATTCAGAACGTTTTTTTTCAGGAGACACGAACATGGCCAGCAGAGGCGTAAACAAGGTTATTCTCGTTg                             >  1:679666/1‑91 (MQ=255)                                                        gTACAATCGCGCGTTTACACTTATTCAGAACGTTTTTTTTCAGGAGACACGAACATGGCCAGCAGAGGCGTAAACAAGGTTATTCTCGTTg                             >  1:395391/1‑91 (MQ=255)                                                                gcgcgTTTACACTTATTCAGAACGTTTTTTTTCAGGAGACACGAACATGGCCAGCAGAGGCGTAAACAAGGTTATTCTCGTTGGTAATCTg                     >  2:215104/1‑91 (MQ=255)                                                                gcgcgTTTACACTTATTCAGAACGTTTTTTTTCAGGAGACACGAACATGGCCAGCAGAGGCGTAAACAAGGTTATTCTCGGTGGTAGTcgt                     >  2:178289/1‑89 (MQ=255)                                                                 cgcgTTTACACTTATTCAGAACGTTTTTTTTCAGGAGACACGAACATGGCCAGCAGAGGCGTAAACAAGGTTATTCTCGTTGGTAATCTgg                    >  1:328516/1‑91 (MQ=255)                                                                  gcgTTTACACTTATTCAGAACGTTTTTTTTCAGGAGACACGAACATGGCCAGCAGAGGCGTAAACAAGGTTATTCTCGTTGGTAATc                       <  2:39358/87‑1 (MQ=255)                                                                  gcgTTTACACTTATTCAGAACGTTTTTTTTCAGGAGACACGAACATGGCCAGCAGAGGCGTAAACAAGGTTATTCTCGTTGGTAATc                       >  1:39358/1‑87 (MQ=255)                                                                      ttACACTTATTCAGAACGTTTTTTTTCAGGAGACACGAACATGGCCAGCAGAGGCGTAAACAAGGTTATTCTCGTTGGTAATCTGGGTCAg               <  2:737973/91‑1 (MQ=255)                                                                      ttACACTTATTCAGAACGTTTTTTTTCAGGAGACACGAACATGGCCAGCAGAGGCGTAAACAAGGTTATTCTCGTTGGTAATCTGGGTCAg               <  2:304531/91‑1 (MQ=255)                                                                           cTTATTCAGAACGTTTTTTTTCAGGAGACACGAACATGGCCAGCAGAGGCGTAAACAAGGTTATTCTCGTTGCTAATCTGGGTCAGGcccc          <  2:199705/91‑1 (MQ=255)                                                                             tATTCAGAACGTTTTTTTTCAGGAGACACGAACATGGCCAGCAGAGGCGTAAACAAGGTTATTCTCGTTGGTAATTTGGGTCAGGACCCgg        >  1:480080/1‑91 (MQ=255)                                                                              aTTCAGAACGTTTTTTTTCAGGAGACACGAACATGGCCAGCAGAGGCGTAAACAAGGTTATTCTCGTTGGTAATCTGGGTCAGGACCCGGa       <  2:470817/91‑1 (MQ=255)                                                                              aTTCAGAACGTTTTTTTTCAGGAGACACGAACATGGCCAGCAGAGGCGTAAACAAGGTTATTCTCGTTGGTAATCTGGGTCAGGACCCGGa       <  2:605757/91‑1 (MQ=255)                                                                               ttCAGAACGTTTTTTTTCAGGAGACACGAACATGGCCAGCAGAGGCGTAAAAAAGGTTATTTTCGTTGGTAATCTGGGTCAGGAACCGGac      >  1:770665/1‑90 (MQ=255)                                                                                   gAACGTTTTTTTTCAGGAGACACGAACATGGCCAGCAGAGGCGTAAACAAGGTTATTCTCGTTGGTAATc                       <  2:205390/70‑1 (MQ=255)                                                                                   gAACGTTTTTTTTCAGGAGACACGAACATGGCCAGCAGAGGCGTAAACAAGGTTATTCTCGTTGGTAATc                       >  1:205390/1‑70 (MQ=255)                                                                                   gAACGTTTTTTTTCAGGAGACACGAACATGGCCAGCAGAGGCGTAAACAAGGTTATTCTCGTTGGTAATCTGGGTCAGGACCCGGAAGTAc  >  1:620929/1‑91 (MQ=255)                                                                                   gAACGTTTTTTTTCAGGAGACACGAACATGGCCAGCAGAGGCGTAAACAAGGTTATTCTCGTTGGTAATCTGGGTCAGGACCCGGAAGTAc  >  1:522497/1‑91 (MQ=255)                                                                                   gAACGTTTTTTTTCAGGAGACACGAACATGGCCAGCAGAGGCGTAAACAAGGTTATTCTCGTTGGTAATCTGGGTCAGGACCCGGAAGTAc  >  1:522464/1‑91 (MQ=255)                                                                                        |                                                                                      CAGGTTTGTTTCCCGGAACCGAGGTCACAACATAGTAAAAGCGCTATTGGTAATGGTACAATCGCGCGTTTACACTTATTCAGAACGATTTTTTTCAGGAGACACGAACATGGCCAGCAGAGGCGTAAACAAGGTTATTCTCGTTGGTAATCTGGGTCAGGACCCGGAAGTAC  >  NZ_CP009273/4263944‑4264116

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑