Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	469,392	T→C	L324P (CTC→CCC)	amtB →	ammonium transporter AmtB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	469,392	0	T	C	100.0%	60.4 / NA	18	L324P (CTC→CCC)	amtB	ammonium transporter AmtB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (9/9);  total (9/9)

ACGCCAGCCTGCGGCTACATTGGGGTTGGCGGCGCGTTGATTATCGGCGTGGTAGCTGGTCTGGCGGGCTTGTGGGGCGTTACCATGCTCAAACGCTTGCTGCGGGTGGATGATCCCTGCGATGTCTTCGGTGTGCACGGCGTTTGTGGCATTGTCGGCTGTATCATGA  >  NZ_CP009273/469304‑469472                                                                                         |                                                                                 aCGCCAGCCTGCGGCTACATTGGGGTTGGCGGCGCGTTGATTATCGGCGTGGTAGCTGGTCTGGCGGGCTTGTGGGGCGTTACCATGCCCa                                                                                >  1:651231/1‑91 (MQ=255)               cTACATTGGGGTTGGCGGCGCGTTGATTATCGGCGTGGTAGCTGGTCTGGCGGGCTTGTGGGGCGTTACCATGCCCAAACGCTTGCTGCgg                                                                  <  2:651231/91‑1 (MQ=255)                tACATTGGGGTTGGCGGCGCGTTGATTATCGGCGTGGTAGCTGGTCTGGCGGGCTTGTGGGGCGTTACCATGCCCAAACGCTTGCTGCggg                                                                 >  1:176965/1‑91 (MQ=255)                      ggggTTGGCGGCGCGTTGATTATCGGCGTGGTAGCTGGTCTGGCGGGCTTGTGGGGCGTTACCATGCCCAAACGCTTGCTGCGGGTGgatg                                                           <  2:617920/91‑1 (MQ=255)                      ggggTTGGCGGCGCGTTGATTATCGGCGTGGTAGCTGGTCTGGCGGGCTTGTGGGGCGTTACCATGCCCAAACGCTTGCTGCGGGTGgatg                                                           <  2:361321/91‑1 (MQ=255)                        ggTTGGCGGCGCGTTGATTATCGGCGTGATAGCTGGTCTGGCGGGCTTGTGGGGCGTTACCATGCCCAAACGCTTGCTGCGGGTGgatgat                                                         >  1:276531/1‑91 (MQ=255)                            ggcggcGCGTTGATTATCGGCGTGGTAGCTGGTCTGGCGGGCTTGTGGGGCGTTTCCATGCCCAAACGCTTGCTGCGGGGGGGTGATTCCt                                                     >  2:441785/1‑91 (MQ=255)                             gcggcgCGTTGATTATCGGCGTGGTAGCTGGTCTGGCGGGCTTGTGGGGCGTTACCATGCCCAAACGCTTGCTGCGGGTGGATGATCCCTg                                                    >  2:737537/1‑91 (MQ=255)                               ggcgCGTTGATTATCGGCGTGGTAGCTGGTCTGGCGGGCTTGTGGGGCGTTACCATGCCCAAACGCTTGCTGCGGGTGGATGATCCCTGCg                                                  <  1:401832/91‑1 (MQ=255)                               ggcgCGTTGATTATCGGCGTGGTAGCTGGTCTGGCGGGCTTGTGGGGCGTTACCATGCCCAAACGCTTGCTGCGGGTGGATGATCCCTGCg                                                  <  1:182890/91‑1 (MQ=255)                                      tGATTATCGGCGTGGTAGCTGGTCTGGCGGGCTTGTGGGGCGTTACCATGCCCAAACGCTTGCTGCGGGTGGATGATCCCTGCGATGTCtt                                           <  2:101100/91‑1 (MQ=255)                                                 gTGGTAGCTGGTCTGGCGGGCTTGTGGGGCGTTACCATGCCCAAACGCTTGCTGCGGGTGGATGATCCCTGCGATGTCTTCGGTGTGCACg                                >  1:121306/1‑91 (MQ=255)                                                              tGGCGGGCTTGTGGGGCGTTACCATGCCCAAACGCTTGCTGCGGGTGGATGATCCCTGCGATGTCTTCGGTGTGCACGGCGTTTGTGGCAt                   >  2:173040/1‑91 (MQ=255)                                                                gCGGGCTTGTGGGGCGTTACCATGCCCAAACGCTTGCTGCGGGTGGATGATCCCTGCGATGTCTTCGGTGTGCACGGCGTTTGTGGCATTg                 <  2:106364/91‑1 (MQ=255)                                                                gCGGGCTTGTGGGGCGTTACCATGCCCAAACGCTTGCTGCGGGTGGATGATCCCTGCGATGTCTTCGGTGTGCACGGCGTTTGTGGCATTg                 <  2:704680/91‑1 (MQ=255)                                                                      ttGTGGGGCGTTACCATGCCCAAACGCTTGCTGCGGGTGGATGATCCCTGCGATGTCTTCGGTGTGCACGGCGTTTGTGGCATTGTCGGCt           <  2:293575/91‑1 (MQ=255)                                                                         tgGGGCGTTACCATGCCCAAACGCTTGCTGCGGGTGGATGATCCCTGCGATGTCTTCGGTGTGCACGGCGTTTGTGGCATTGTCGGCTGTa        >  2:358651/1‑91 (MQ=255)                                                                               gTTACCATGCCCAAACGCTTGCTGCGGGTGGATGATCCCTGCGATGTCTTCGGTGTGCACGGCGTTTGTGGCATTGTCGGCTGTATCATGa  >  1:183197/1‑91 (MQ=255)                                                                                         |                                                                                 ACGCCAGCCTGCGGCTACATTGGGGTTGGCGGCGCGTTGATTATCGGCGTGGTAGCTGGTCTGGCGGGCTTGTGGGGCGTTACCATGCTCAAACGCTTGCTGCGGGTGGATGATCCCTGCGATGTCTTCGGTGTGCACGGCGTTTGTGGCATTGTCGGCTGTATCATGA  >  NZ_CP009273/469304‑469472

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑