Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	4,359,587	G→T	R215S (CGT→AGT)	yjeH ←	L‑methionine/branched‑chain amino acid transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	4,359,587	0	G	T	100.0%	88.5 / NA	24	R215S (CGT→AGT)	yjeH	L‑methionine/branched‑chain amino acid transporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (18/6);  total (18/6)

CGAAGTGTAAGACGACTACCGTACAGCCCCAGTAGACTAATCCTGCCAGCAGCAGACCAATCATCAAAGCACGAGGAAAATCACGCTCTGGATTTTTAAATTCCGAGGCGAGATGGGCAAATGCCTCCAGACCGACAAAACACCAGAACATCACTGATAACGCAGCAAAT  >  NZ_CP009273/4359503‑4359672                                                                                     |                                                                                      cGAAGTGTAAGACGACTACCGTACAGCCCCAGTAGACTAATCCTGCCAGCAGCAGACCAATCATCAAAGCACGAGGAAAATCACTCTCTgg                                                                                 >  1:46052/1‑91 (MQ=255)               acTACCGTACAGCCCCAGTAGACTAATCCTGCCAGCAGCAGACCAATCATCAAAGCACGAGGAAAATCACTCTCTGGATTTTTAAATTCCg                                                                   >  2:841286/1‑91 (MQ=255)               acTACCGTACAGCCCCAGTAGACTAATCCTGCCAGCAGCAGACCAATCATCAAAGCACGAGGAAAATCACTCTCTGGATTTTTAAATTCCg                                                                   >  2:458324/1‑91 (MQ=255)               acTACCGTACAGCCCCAGTAGACTAATCCTGCCAGCAGCAGACCAATCATCAAAGCACGAGGAAAATCACTCTCTGGATTTTTAAATTCCg                                                                   <  2:777528/91‑1 (MQ=255)               acTACCGTACAGCCCCAGTAGACTAATCCTGCCAGCAGCAGACCAATCATCAAAGCACGAGGAAAATCACTCTCTGGATTTTTAAATTCCg                                                                   >  2:381773/1‑91 (MQ=255)                          gCCCCAGTAGACTAATCCTGCCAGCAGCAGACCAATCATCAAAGCACGAGGAAAATCACTCTCTGGATTTTTAAATTCCGAGGCGAGATgg                                                        >  2:745236/1‑91 (MQ=255)                          gCCCCAGTAGACTAATCCTGCCAGCAGCAGACCAATCATCAAAGCACGAGGAAAATCACTCTCTGGATTTTTAAATTCCGAGGCGAGATgg                                                        >  1:51694/1‑91 (MQ=255)                          gCCCCAGTAGACTAATCCTGCCAGCAGCAGACCAATCATCAAAGCACGAGGAAAATCACTCTCTGGATTTTTAAATTCCGAGGCGAGATgg                                                        >  1:383461/1‑91 (MQ=255)                            cccAGTAGACTAATCCTGCCAGCAGCAGACCAATCATCAAAGCACGAGGAAAATCACTCTCTGGATTTTTAAATTCCGAGGCGAGATGGGc                                                      >  1:244289/1‑91 (MQ=255)                               aGTAGACTAATCCTGCCAGCAGCAGACCAATCATCAAAGCACGAGGAAAATCACTCTCTGGATTTTTAAATTCCGAGGCGAGATGGGCaaa                                                   >  1:690727/1‑91 (MQ=255)                                     cTAATCCTGCCAGCAGCAGACCAATCATCAAAGCACGAGGAAAATCACTCTCTGGATTTTTAAATTCCGAGGCGAGATGGGCAAATGCCTc                                             >  1:11756/1‑91 (MQ=255)                                       aaTCCTGCCAGCAGCAGACCAATCATCAAAGCACGAGGAAAATCACTCTCTGGATTTTTAAATTCCGAGGCGAGATGGGCAAATGCCTCCa                                           >  1:824093/1‑91 (MQ=255)                                       aaTCCTGCCAGCAGCAGACCAATCATCAAAGCACGAGGAAAATCACTCTCTGGATTTTTAAATTCCGAGGCGAGATGGGCAAATGCCTCCa                                           >  2:765473/1‑91 (MQ=255)                                            tGCCAGCAGCAGACCAATCATCAAAGCACGAGGAAAATCACTCTCTGGATTTTTAAATTCCGAGGCGAGATGGGCAAATGCCTCCAGACCg                                      >  1:416769/1‑91 (MQ=255)                                             gCCAGCAGCAGACCAATCATCAAAGCACGAGGAAAATCACTCTCTGGATTTTTAAATTCCGAGGCGAGATGGGCAAATGCCTCCAGACCGa                                     >  1:618961/1‑91 (MQ=255)                                                 gcagcagACCAATCATCAAAGCACGAGGAAAATCACTCTCTGGATTTTTAAATTCCGAGGCGAGATGGGCAAATGCCTCCAGACCGACaaa                                 <  2:824093/91‑1 (MQ=255)                                                    gcagACCAATCATCAAAGCACGAGGAAAATCACTCTCTGGATTTTTAAATTCCGAGGCGAGATGGGCAAATGCCTCCAGACCGACAAAaca                              >  2:698011/1‑91 (MQ=255)                                                      agACCAATCATCAAAGCACGAGGAAAATCACTCTCTGGATTTTTAAATTCCGAGGCGAGATGGGCAAATGCCTCCAGACCGACAAAACAcc                            <  1:24311/91‑1 (MQ=255)                                                        aCCAATCATCAAAGCACGAGGAAAATCACTCTCTGGATTTTTAAATTCCGAGGCGAGATGGGCAAATGCCTCCAGACCGACAAAACACCAg                          <  1:409825/91‑1 (MQ=255)                                                          cAATCATCAAAGCACGAGGAAAATCACTCTCTGGATTTTTAAATTCCGAGGCGAGATGGGCAAATGCCTCCAGACCGACAAAACACCAGaa                        <  1:745236/91‑1 (MQ=255)                                                           aaTCATCAAAGCACGAGGAAAATCACTCTCTGGATTTTTAAATTCCGAGGCGAGATGGGCAAATGCCTCCAGACCGACAAAACACCAGAAc                       >  2:457219/1‑91 (MQ=255)                                                           aaTCATCAAAGCACGAGGAAAATCACTCTCTGGATTTTTAAATTCCGAGGCGAGATGGGCAAATGCCTCCAGACCGACAAAACACCAGAAc                       >  1:161277/1‑91 (MQ=255)                                                                 caAAGCACGAGGAAAATCACTCTCTGGATTTTTAAATTCCGAGGCGAGATGGGCAAATGCCTCCAGACCGACAAAACACCAGAACATCACt                 >  2:607396/1‑91 (MQ=255)                                                                                aTCACTCTCTGGATTTTTAAATTCCGAGGCGAGATGGGCAAATGCCTCCAGACCGACAAAACACCAGAACATCACTGATAACGCAGCAAAt  <  1:607396/91‑1 (MQ=255)                                                                                     |                                                                                      CGAAGTGTAAGACGACTACCGTACAGCCCCAGTAGACTAATCCTGCCAGCAGCAGACCAATCATCAAAGCACGAGGAAAATCACGCTCTGGATTTTTAAATTCCGAGGCGAGATGGGCAAATGCCTCCAGACCGACAAAACACCAGAACATCACTGATAACGCAGCAAAT  >  NZ_CP009273/4359503‑4359672

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑