Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	4,377,323	T→C	D622G (GAC→GGC)	mscM ←	miniconductance mechanosensitive channel MscM

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	4,377,323	0	T	C	100.0%	90.1 / NA	26	D622G (GAC→GGC)	mscM	miniconductance mechanosensitive channel MscM
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (12/14);  total (12/14)

GGCCTTTTTCAGGCTGAGGGTGACCACCGCCAGCGCACCGCAAATGAGGATAAAGCAAAGCCGTCCCAGCGAACCGGAGAATTCACGGTCGTCGAGGTTATCGAACATCATCAGCGCCATAATCAGCGGCACAATAAGCCCGATGCTCATCAGGTAGTAGCGCAT  >  NZ_CP009273/4377235‑4377399                                                                                         |                                                                             ggCCTTTTTCAGGCTGAGGGTGACCACCGCCAGCGCACCGCAAATGAGGATAAAGCAAAGCCGTCCCAGCGAACCGGAGAATTCACGgccg                                                                            <  1:715169/91‑1 (MQ=255)      ttttCAGGCTGAGGGTGACCACCGCCAGCGCACCGCAACTGAGGATAAAGCAAAGCCGTCCCAGCGAACCGGAGAATTCACGGCCGTCGAg                                                                       >  1:109961/1‑91 (MQ=255)               tcaGGGTGGCCACCGCCAGCGCACCGCAAATGAGGATAAAGCAAAGCCGTCCGAGCGAACCGGAGAATTCACGGCCGTCGATGGTATCGaa                                                              <  2:322359/89‑1 (MQ=255)                gAGGGTGACCACCGCCAGCGCACCGCAAATGAGGATAAAGCAAAGCCGTCCCAGCGAACCGGAGAATTCACGGCCGTCGAGGTTATCGAAc                                                             >  1:404235/1‑91 (MQ=255)                      gACCACCGCCAGCGCACCGCAAATGAGGATAAAGCAAAGCCGTCCCAGCGAACCGGAGAATTCACGGCCGTCGAGGTTATCGAACatcatc                                                       >  1:358739/1‑91 (MQ=255)                                 gcgcACCGCAAATGAGGATAAAGCAAAGCCGTCCCAGCGAACCGGAGAATTCACGGCCGTCGAGGTTATCGAACATCATCAGCGCCATAAt                                            >  1:417383/1‑91 (MQ=255)                                     aCCGCAAATGAGGATAAAGCAAAGCCGTCCCAGCGAACCGGAGAATTCACGGCCGTCGAGGTTATCGAACATCATCAGCGCCATAATCAGc                                        <  1:150994/91‑1 (MQ=255)                                     aCCGCAAATGAGGATAAAGCAAAGCCGTCCCAGCGAACCGGAGAATTCACGGCCGTCGAGGTTATCGAACATCATCAGCGCCATAATCAGc                                        >  1:383946/1‑91 (MQ=255)                                     aCCGCAAATGAGGATAAAGCAAAGCCGTCCCAGCGAACCGGAGAATTCACGGCCGTCGAGGTTATCGAACATCATCAGCGCCATAATCAGc                                        <  1:55981/91‑1 (MQ=255)                                     aCCGCAAATGAGGATAAAGCAAAGCCGTCCCAGCGAACCGGAGAATTCACGGCCGTCGAGGTTATCGAACATCATCAGCGCCATAATCAGc                                        <  1:620001/91‑1 (MQ=255)                                        gCAAATGAGGATAAAGCAAAGCCGTCCCAGCGAACCGGAGAATTCACGGCCGTCGAGGTTATCGAACATCATCAGCGCCATAATCAGCGGc                                     <  1:784081/91‑1 (MQ=255)                                              gAGGATAAAGCAAAGCCGTCCCAGCGAACCGGAGAATTCACGGCCGTCGAGGTTATCGAACATCATCAGCGCCATAATCAGCGGCACAATa                               >  2:351146/1‑91 (MQ=255)                                                ggATAAAGCAAAGCCGTCCCAGCGAACCGGAGAATTCACGGCCGTCGAGGTTATCGAACATCATCAGCGCCATAATCAGCGGCACAATAAg                             >  2:335073/1‑91 (MQ=255)                                                     aagcaaagcCGTCCCAGCGAACCGGAGAATTCACGGCCGTCGAGGTTATCGAACATCATCAGCGCCATAATCAGCGGCACAATAAGCCCGa                        >  1:621244/1‑91 (MQ=255)                                                     aagcaaagcCGTCCCAGCGAACCGGAGAATTCACGGCCGTCGAGGGTATCGAACATCATCAGCGCCATAATCAGCGGCACAATAAGCCCGa                        <  2:128672/91‑1 (MQ=255)                                                      agcaaagcCGTCCCAGCGAACCGGAGAATTCACGGCCGTCGAGGTTATCGAACATCATCAGCGCCATAATCAGCGGCACAATAAGCCCGAt                       <  1:710713/91‑1 (MQ=255)                                                       gcaaagcCGTCCCAGCGAACCGGAGAATTCACGGCCGTCGAGGTTATCGAACATCATCAGCGCCATAATCAGCGGCACAATAAGCCCGATg                      <  2:741804/91‑1 (MQ=255)                                                          aagcCGTCCCAGCGAACCGGAGAATTCACGGCCGTCGAGGTTATCGAACATCATCAGCGCCATAATCAGCGGCACAATAAGCCCGATGCtc                   <  1:465036/91‑1 (MQ=255)                                                          aagcCGTCCCAGCGAACCGGAGAATTCACGGCCGTCGAGGTTATCGAACATCATCAGCGCCATAATCAGCGGCACAATAAGCCCGATGCtc                   >  2:330777/1‑91 (MQ=255)                                                          aagcCGTCCCAGCGAACCGGAGAATTCACGGCCGTCGAGGTTATCGAACATCATCAGCGCCATAATCAGCGGCACAATAAGCCCGATGCtc                   <  1:42474/91‑1 (MQ=255)                                                            gcCGTCCCAGCGAACCGGAGAATTCACGGCCGTCGAGGTTATCGAACATCATCAGCGCCATAATCAGCGGCACAATAAGCCCGATGCtcat                 <  1:737026/91‑1 (MQ=255)                                                            gcCGTCCCAGCGAACCGGAGAATTCACGGCCGTCGAGGTTATCGAACATCATCAGCGCCATAATCAGCGGCACAATAAGCCCGATGCtcat                 <  2:705057/91‑1 (MQ=255)                                                               gTCCCAGCGAACCGGAGAATTCACGGCCGTCGAGGTTATCGAACATCATCAGCGCCATAATCAGCGGCACAATAAGCCCGATGCTCATCAg              >  2:23256/1‑91 (MQ=255)                                                                    aGCGAACCGGAGAATTCACGGCCGTCGAGGTTATCGAACATCATCAGCGCCATAATCAGCGGCACAATAAGGCCGATGCTCATCAGgtagt         >  1:666532/1‑91 (MQ=255)                                                                    aGCGAACCGGAGAATTCACGGCCGTCGAGGTTATCGAACATCATCAGCGCCATAATCAGCGGCACAATAAGCCCGATGCTCATCAGgtagt         >  1:282637/1‑91 (MQ=255)                                                                      cGAACCGGAGAATTCACGGCCGTCGAGGTTATCGAACATCATCAGCGCCATAATCAGCGGCACAATAAGCCCGATGCGCATCAGGtagtag       <  2:404235/91‑1 (MQ=255)                                                                           cGGAGAATTCACGGCCGTCGAGGTTATCGAACATCATCAGCGCCATAATCAGCGGCACAATAAGCCCGATGCTCATCAGGTAGTAGCGCAt  <  1:751461/91‑1 (MQ=255)                                                                                         |                                                                             GGCCTTTTTCAGGCTGAGGGTGACCACCGCCAGCGCACCGCAAATGAGGATAAAGCAAAGCCGTCCCAGCGAACCGGAGAATTCACGGTCGTCGAGGTTATCGAACATCATCAGCGCCATAATCAGCGGCACAATAAGCCCGATGCTCATCAGGTAGTAGCGCAT  >  NZ_CP009273/4377235‑4377399

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑