Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	4,395,911	T→C	intergenic (+109/‑96)	purA → / → nsrR	adenylosuccinate synthase/nitric oxide‑sensing transcriptional repressor NsrR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	4,395,911	0	T	C	100.0%	104.4 / NA	28	intergenic (+109/‑96)	purA/nsrR	adenylosuccinate synthase/nitric oxide‑sensing transcriptional repressor NsrR
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (21/7);  total (21/7)

CCGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCTTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTT  >  NZ_CP009273/4395833‑4395997                                                                               |                                                                                       ccGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAAGCCTCGCTTTTTCCCTTCCCCGAAc                                                                            <  1:614662/91‑1 (MQ=255)  cGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTCCCTTCCCCGAACt                                                                           <  2:65288/91‑1 (MQ=255)             tgtgATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTCCCTTCCCCGAACTGAAATAAATTa                                                                <  2:510701/91‑1 (MQ=255)                      ttGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTCCCTTCCCCGAACTGAAATAAATTAGCGACACAg                                                       >  2:129071/1‑91 (MQ=255)                      ttGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTCCCTTCCCCGAACTGAAATAAATTAGCGACACAg                                                       >  1:579327/1‑91 (MQ=255)                        gCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTCCCTTCCCCGAACTGAAATAAATTAGCGACACAGCt                                                     >  1:784291/1‑91 (MQ=255)                        gCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTCCCTTCCCCGAACTGAAATAAATTAGCGACACAGCt                                                     <  2:350607/91‑1 (MQ=255)                        gCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTCCCTTCCCCGAACTGAAATAAATTAGCGACACAGCt                                                     >  1:817587/1‑91 (MQ=255)                         cTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTCCCTTCCCCGAACTGAAATAAATTAGCGACACAGCtt                                                    <  1:308722/91‑1 (MQ=255)                           gtgtCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTCCCTTCCCCGAACTGAAATAAATTAGCGACACAGCTtgt                                                  >  2:560602/1‑91 (MQ=255)                           gtgtCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTCCCTTCCCCGAACTGAAATAAATTAGCGACACAGCTtgt                                                  >  1:646196/1‑91 (MQ=255)                           gtgtCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTCCCTTCCCCGAACTGAAATAAATTAGCGACACAGCTtgt                                                  <  1:94237/91‑1 (MQ=255)                           gtgtCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTCCCTTCCCCGAACTGAAATAAATTAGCGACACAGCTtgt                                                  >  1:835026/1‑91 (MQ=255)                             gtCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTCCCTTCCCCGAACTGAAATAAATTAGCGACACAGCTTGTgg                                                >  1:205305/1‑91 (MQ=255)                                gggTAGGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTCCCTTCCCCGAACTGAAATAAATTAGCGACACAGCTTGTGGCTg                                             >  1:535744/1‑91 (MQ=255)                                     aGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTCCCTTCCCCGAACTGAAATAAATTAGCGACACAGCTTGTGGCTGGTTTa                                        >  1:45572/1‑91 (MQ=255)                                       ccATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTCCCTTCCCCGAACTGAAATAAATTAGCGACACAGCTTGTGGCTGGTTTatc                                      >  2:38305/1‑91 (MQ=255)                                                      acacAGTGTTAAATCCTCGCTTTTTCCCTTCCCCGAACTGAAATAAATTAGCGacac                                                         <  1:492789/57‑1 (MQ=255)                                                      acacAGTGTTAAATCCTCGCTTTTTCCCTTCCCCGAACTGAAATAAATTAGCGacac                                                         >  2:492789/1‑57 (MQ=255)                                                      acacAGTGTTAAATCCTCGCTTTTTCCCTTCCCCGAACTGAAATAAATTAGCGACACAGCTTGTGGCTGGGTTTTCATCAATATAAATGTa                       >  1:619966/1‑91 (MQ=255)                                                           gtgtTAAATCCTCGCTTTTTCCCTTCCCCGAACTGAAATAAATTAGCGACACAGCTTGTGGCTGGTTTATCATCAATATAAATGTAttttt                  >  1:718419/1‑91 (MQ=255)                                                           gtgtTAAATCCTCGCTTTTTCCCTTCCCCGAACTGAAATAAATTAGCGACACAGCTTGTGGCTGGTTTATCATCAATATAAATGTAttttt                  >  2:302948/1‑91 (MQ=255)                                                           gtgtTAAATCCTCGCTTTTTCCCTTCCCCGAACTGAAATAAATTAGCGACACAGCTTGTGGCTGGGTTATCATCAATATAAATGTAttttt                  >  2:612982/1‑91 (MQ=255)                                                           gtgtTAAATCCTCGCTTTTTCCCTTCCCCGAACTAAAATAAATTAGCGACACAGCTTGTGGCTGGTTTATCATCAATATAAATTGAttttt                  >  2:43835/1‑91 (MQ=255)                                                                aaaTCCTCGCTTTTTCCCTTCCCCGAACTGAAATAAATTAGCGACACAGCTTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCg             >  1:579248/1‑91 (MQ=255)                                                                     cTCGCTTTTTCCCTTCCCCGAACTGAAATAAATTAGCGACACAGCTTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTc        >  1:409404/1‑91 (MQ=255)                                                                       cGCTTTTTCCCTTCCCCGAACTGAAATAAATTAGCGACACAGCTTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTccc      >  1:647523/1‑91 (MQ=255)                                                                       cGCTTTTTCCCTTCCCCGAACTGAAATAAATTAGCGACACAGCTTGTGGCTGGGTTTTCATCCATATAAATGTATTTTTTTCCGATTTccc      >  2:107491/1‑91 (MQ=255)                                                                           ttttcCCTTCCCCGAACTGAAATAAATTAGCGACACAGCTTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCtttt  <  1:332929/91‑1 (MQ=255)                                                                               |                                                                                       CCGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCTTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTT  >  NZ_CP009273/4395833‑4395997

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑