Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	4,493,312	A→G	intergenic (‑46/‑563)	insG ← / → nanX	IS4‑like element IS4 family transposase/MFS transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	4,493,312	0	A	G	100.0%	92.4 / NA	25	intergenic (‑46/‑563)	insG/nanX	IS4‑like element IS4 family transposase/MFS transporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (15/10);  total (15/10)

TCAAGAGCCTGTCCAATGTGCATAAAAAAATCCGGAAACAAGTGAGCGTTTCCGGATTCTTACACAGCCACTGGATCGGTCAACTGATCCTTAACTGATCGGCATTAATCTTGGTTCTGGTGTTTGTAACAAACTATCAGCTACAAAAATATGCTCAATTTGTGACAT  >  NZ_CP009273/4493226‑4493393                                                                                       |                                                                                  tCAAGAGCCTGTCCAATGTGCATAAAAAAATCCGGAAACAAGTGAGCGTTTCCGGATTCTTACACAGCCACTGGATCGGTCAACTGGTCCt                                                                               >  1:755520/1‑91 (MQ=255)   aaGAGCCTGTCCAATGTGCATAAAAAAATCCGGAAACAAGTGAGCGTTTCCGGATTCTTACACAGCCACTGGATCGGTCAACTGGTCCTTa                                                                             >  1:597626/1‑91 (MQ=255)         cTGTCCAATGTGCATAAAAAAATCCGGAAACAAGTGAGCGTTTCCGGATTCTTACACAGCCACTGGATCGGTCAACTGGTCCTTAACTGAt                                                                       >  1:136714/1‑91 (MQ=255)         cTGTCCAATGTGCATAAAAAAATCCGGAAACAAGTGAGCGTTTCCGGATTCTTACACAGCCACTGGATCGGTCAACTGGTCCTTAACTGAt                                                                       >  2:692453/1‑91 (MQ=255)          tGTCCAATGTGCATAAAAAAATCCGGAAACAAGTGAGCGTTTCCGGATTCTTACACAGCCACTGGATCGGTCAACTGGTCCTTAACTGATc                                                                      <  2:802477/91‑1 (MQ=255)          tGTCCAATGTGCATAAAAAAATCCGGAAACAAGTGAGCGTTTCCGGATTCTTACACAGCCACTGGATCGGTCAACTGGTCCTTAACTGATc                                                                      <  2:120348/91‑1 (MQ=255)                aTGTGCATAAAAAAATCCGGAAACAAGTGAGCGTTTCCGGATTCTTACACAGCCACTGGATCGGTCAACTGGTCCTTAACTGATCGGCAtt                                                                <  1:249546/91‑1 (MQ=255)                 tgtgCATAAAAAAATCCGGAAACAAGTGAGCGTTTCCGGATTCTTACACAGCCACTGGATCGGTCAACTGGTCCTTAACTGATCGGCATTa                                                               <  2:597626/91‑1 (MQ=255)                     cATAAAAAAATCCGGAAACAAGTGAGCGTTTCCGGATTCTTACACAGCCACTGGATCGGTCAACTGGTCCTTAACTGATc                                                                      >  2:499798/1‑80 (MQ=255)                     cATAAAAAAATCCGGAAACAAGTGAGCGTTTCCGGATTCTTACACAGCCACTGGATCGGTCAACTGGTCCTTAACTGATc                                                                      <  1:499798/80‑1 (MQ=255)                     cATAAAAAAATCCGGAAACAAGTGAGCGTTTCCGGATTCTTACACAGCCACTGGATCGGTCAACTGGTCCTTAACTGATCGGCATTAATCt                                                           >  2:457374/1‑91 (MQ=255)                                      aCAAGTGAGCGTTTCCGGATTCTTACACAGCCACTGGATCGGTCAACTGGTCCTTAACTGATCGGCATTAATCTTGGTTCTGGTGTTTGTa                                          <  2:774048/91‑1 (MQ=255)                                             aGCGTTTCCGGATTCTTACACAGCCACTGGATCGGTCAACTGGTCCTTAACTGATCGGCATTAATCTTGGTTCTGGTGTTTGTAACAAACt                                   >  1:624012/1‑91 (MQ=255)                                              gCGTTTCCGGATTCTTACACAGCCACTGGATCGGTCAACTGGTCCTTAACTGATCGGCATTAATCTTGGTTCTGGTGTTTGTAACAAACTa                                  >  1:262166/1‑91 (MQ=255)                                                                acaGCCACTGGATCGGTCAACTGGTCCTTAACTGATCGGCATTAATCTTGGTTCTGGTGTTTGTAACAAACTATCAGCTACAAAAATATGc                <  1:723560/91‑1 (MQ=255)                                                                   gCCACTGGATCGGTTAACTGGCCCTTAACTGATCGGCATTAATCTTGGTTCTGGTGTTTGTAACAAACTATCAGCTACAAAAATATGCTCa             >  2:485947/1‑91 (MQ=255)                                                                   gCCACTGGATCGGTCAACTGGTCCTTAACTGATCGGCATTAATCTTGGTTCTGGTGTTTGTAACAAACTATCAGCTACAAAAATATGCTCa             <  2:680499/91‑1 (MQ=255)                                                                             cGGTCAACTGGTCCTTAACTGATCGGCATTAATCTTGGTTCTGGTGTTTGTAACAAACTATCAGCTACAAAAATATGCTCAATTTGTGACa   <  1:112316/91‑1 (MQ=255)                                                                             cGGTCAACTGGTCCTTAACTGATCGGCATTAATCTTGGTTCTGGTGTTTGTAACAAACTATCAGCTACAAAAATATGCTCAATTTGTGACa   >  2:846661/1‑91 (MQ=255)                                                                             cGGTCAACTGGTCCTTAACTGATCGGCATTAATCTTGGTTCTGGTGTTTGTAACAAACTATCAGCTACAAAAATATGCTCAATTTGTGACa   >  1:774423/1‑91 (MQ=255)                                                                             cGGTCAACTGGTCCTTAACTGATCGGCATTAATCTTGGTTCTGGTGTTTGTAACAAACTATCAGCTACAAAAATATGCTCAATTTGTGACa   >  1:198099/1‑91 (MQ=255)                                                                              ggTCAACTGGTCCTTAACTGATCGGCATTAATCTTGGTTCTGGTGTTTGTAACAAACTATCAGCTACAAAAATATGCTCAATTTGTGACAt  >  2:335534/1‑91 (MQ=255)                                                                              ggTCAACTGGTCCTTAACTGATCGGCATTAATCTTGGTTCTGGTGTTTGTAACAAACTATCAGCTACAAAAATATGCTCAATTTGTGACAt  >  1:298305/1‑91 (MQ=255)                                                                                   aCTGGTCCTTAACTGATCGGCATTAATCTTGGTTCTGGTGTTTGTAACAAACTATCAGCTACAAAAATATGCTCAATTTGTGa     <  2:193724/83‑1 (MQ=255)                                                                                   aCTGGTCCTTAACTGATCGGCATTAATCTTGGTTCTGGTGTTTGTAACAAACTATCAGCTACAAAAATATGCTCAATTTGTGa     >  1:193724/1‑83 (MQ=255)                                                                                       |                                                                                  TCAAGAGCCTGTCCAATGTGCATAAAAAAATCCGGAAACAAGTGAGCGTTTCCGGATTCTTACACAGCCACTGGATCGGTCAACTGATCCTTAACTGATCGGCATTAATCTTGGTTCTGGTGTTTGTAACAAACTATCAGCTACAAAAATATGCTCAATTTGTGACAT  >  NZ_CP009273/4493226‑4493393

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑