Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	4,501,175	T→C	I34V (ATC→GTC)	fecE ←	Fe(3+) dicitrate ABC transporter ATP‑binding protein FecE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	4,501,175	0	T	C	100.0%	71.6 / NA	20	I34V (ATC→GTC)	fecE	Fe(3+) dicitrate ABC transporter ATP‑binding protein FecE
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (10/10);  total (10/10)

GAGAAATACGGTGCCAGACTGCGGCATTAAAAGCCGCGAAAAACAGTTTAACAGCGTCGATTTCCCGCAACCGTTAGGACCGATCAGGGCGGTGATCTTCCCCGTTGGCAGTGAGAGTGAAACGTCGTTAAGTACCTTGTCTGTCC  >  NZ_CP009273/4501092‑4501237                                                                                    |                                                               gagaAATACGGTGCCAGACTGCGGCATTAAAAGCCGCGAAAAACAGTTTAACAGCGTCGATTTCCCGCAACCGTTAGGACCGACCAGGGCg                                                         >  2:374152/1‑91 (MQ=255)       tACGGTGCCAGACTGCGGCATTAAAAGCCGCGAAAAACAGTTTAACAGCGTCGATTTCCCGCAACCGTTAGGACCGACCAGGGCGGTGATc                                                   <  2:551591/91‑1 (MQ=255)             gCCAGACTGCGGCATTAAAAGCCGCGAAAAACAGTTTAACAGCGTCGATTTCCCGCAACCGTTAGGACCGACCAGGGCGGTGATCTTcccc                                             <  2:431070/91‑1 (MQ=255)                 gACTGCGGCATTAAAAGCCGCGAAAAACAGTTTAACAGCGTCGATTTCCCGCAACCGTTAGGACCGACCAGGGCGGTGATCTTCCCCGTTg                                         >  1:615422/1‑91 (MQ=255)                     gCGGCATTAAAAGCCGCGAAAAACAGTTTAACAGCGTCGATTTCCCGCAACCGTTAGGACCGACCAGGGCgg                                                        >  1:300292/1‑72 (MQ=255)                     gCGGCATTAAAAGCCGCGAAAAACAGTTTAACAGCGTCGATTTCCCGCAACCGTTAGGACCGACCAGGGCgg                                                        <  2:300292/72‑1 (MQ=255)                     gCGGCATTAAAAGCCGCGAAAAACAGTTTAACAGCGTCGATTTCCCGCAACCGTTAGGACCGACCAGGGCGGTGATCTTCCCCGTTGGCAg                                     >  2:5248/1‑91 (MQ=255)                     gCGGCATTAAAAGCCGCGAAAAACAGTTTAACAGCGTCGATTTCCCGCAACCGTTAGGACCGACCAGGGCGGTGATCTTCCCCGTTGGCAg                                     >  1:239525/1‑91 (MQ=255)                                  ccGCGAAAAACAGTTTAACAGCGTCGATTTCCCGCAACCGTTAGGACCGACCAGGGCGGTGATCTTCCCCGTTGGCAGTGAGAGTGAAAcg                        >  1:167310/1‑91 (MQ=255)                                   cgcgAAAAACAGTTTAACAGCGTCGATTTCCCGCAACCGTTAGGACCGACCAGGGCGGTGATCTTCCCCGTTGGCAGTGAGAGTGAAAcgt                       <  1:544031/91‑1 (MQ=255)                                    gcgAAAAACAGTTTAACAGCGTCGATTTCCCGCAACCGTTAGGACCGACCAGGGCGGTGATCTTCCCCGTTGGCAGTGAGAGTGAAAcgtc                      >  2:578905/1‑91 (MQ=255)                                         aaaCAGTTTAACAGCGTCGATTTCCCGCAACCGTTAGGACCGACCAGGGCGGTGATCTTCCCCGTTGGCAGTGAGAGTGAAACGTCGTTaa                 <  1:71793/91‑1 (MQ=255)                                                   aCAGCGTCGATTTCCCGCAACCGTTAGGACCGACCAGGGCGGTGATCTTCCCCGTTGGCAGTGAGAGTGAAACGTCGTTAAGTACCTtgtc       <  1:72961/91‑1 (MQ=255)                                                    cAGCGTCGATTTCCCGCAACCGTTAGGACCGACCAGGGCGGTGATCTTCCCCGTTGGCAGTGAGAGTGAAACGTCGTTAAGTACCTtgtct      <  1:741964/91‑1 (MQ=255)                                                    cAGCGTCGATTTCCCGCAACCGTTAGGACCGACCAGGGCGGTGATCTTCCCCGTTGGCAGTGAGAGTGAAACGTCGTTAAGTACCTtgtct      <  1:222968/91‑1 (MQ=255)                                                      gCGTCGATTTCCCGCAACCGTTAGGACCGACCAGGGCGGTGATCTTCCCCGTTGGCAGTGAGAGTGAAACGTCGTTAAGTACCTtgtctgt    <  1:751078/91‑1 (MQ=255)                                                        gTCGATTTCCCGCAACCGTTAGGACCGACCAGGGCGGTGATCTTCCCCGTTGGCAGTGAGAGTGAAACGTCGTTAAGTACCTTGTCTGTcc  >  2:594905/1‑91 (MQ=255)                                                        gTCGATTTCCCGCAACCGTTAGGACCGACCAGGGCGGTGATCTTCCCCGTTGGCAGTGAGAGTGAAACGTCGTTAAGTACCTTGTCTGTcc  >  2:359165/1‑91 (MQ=255)                                                        gTCGATTTCCCGCAACCGTTAGGACCGACCAGGGCGGTGATCTTCCCCGTTGGCAGTGAGAGTGAAACGTCGTTAAGTACCTTGTCTGTcc  <  2:147268/91‑1 (MQ=255)                                                        gTCGATTTCCCGCAACCGTTAGGACCGACCAGGGCGGTGATCTTCCCCGTTGGCAGTGAGAGTGAAACGTCGTTAAGTACCTTGTCTGTcc  >  1:776004/1‑91 (MQ=255)                                                                                    |                                                               GAGAAATACGGTGCCAGACTGCGGCATTAAAAGCCGCGAAAAACAGTTTAACAGCGTCGATTTCCCGCAACCGTTAGGACCGATCAGGGCGGTGATCTTCCCCGTTGGCAGTGAGAGTGAAACGTCGTTAAGTACCTTGTCTGTCC  >  NZ_CP009273/4501092‑4501237

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑