Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	4,545,596	T→C	E181G (GAG→GGG)	yjiC ←	YjfZ/YjiC family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	4,545,596	0	T	C	100.0%	196.7 / NA	51	E181G (GAG→GGG)	yjiC	YjfZ/YjiC family protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (32/19);  total (32/19)

ATGCGGCAAGGGCGAATCCGGAAGAATTTGCTACCCCTGTTACCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCTCATTGGTCCATCTTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAACCAGGAACATTGCAACCAG  >  NZ_CP009273/4545513‑4545683                                                                                    |                                                                                        aTGCGGCAAGGGCGAATCCGGAAGAATTTGCTACCCCTGTTACCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCCCATTGGt                                                                                  >  1:366214/1‑91 (MQ=255) aTGCGGCAAGGGCGAATCCGGAAGAATTTGCTACCCCTGTTACCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCCCATTGGt                                                                                  >  2:293646/1‑91 (MQ=255)                   cGGAAGAATTTGCTACCCCTGTTACCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCCCATTGGTCCATCTTTTTGCCTgaga                                                                <  1:831264/91‑1 (MQ=255)                     gaagaaTTTGCTACCCCTGTTACCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCCCATTGGTCCATCTTTTTGCCTGAGaa                                                               >  1:109177/1‑90 (MQ=255)                     gaagaaTTTGCTACCCCTGTTACCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCCCATTGGTCCATCTTTTTGCCTGAGaa                                                               <  2:109177/90‑1 (MQ=255)                           tttGCTACCCCTGTTACCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCCCATTGGTCCATCTTTTTGCCTGAGAAGCTGGaa                                                        <  1:499907/91‑1 (MQ=255)                           tttGCTACCCCTGTTACCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCCCATTGGTCCATCTTTTTGCCTGAGAAGCTGGaa                                                        <  2:449238/91‑1 (MQ=255)                            ttGCTACCCCTGTTACCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCCCATTGGTCCATCTTTTTGCCTGAGAAGCTGGAAg                                                       <  1:344690/91‑1 (MQ=255)                               cTACCCCTGTTACCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCCCATTGGTCCATCTTTTTGCCTGAGAAGCTGGAAGGGt                                                    <  1:207097/91‑1 (MQ=255)                                  ccccTGTTACCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCCCATTGGTCCATCTTTTTGCCTGAGAAGCTGGAAGGGTTg                                                  <  1:387230/90‑1 (MQ=255)                                  ccccTGTTACCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCCCATTGGTCCATCTTTTTGCCTGAGAAGCTGGAAGGGTTg                                                  >  2:387230/1‑90 (MQ=255)                                         tACCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCCCATTGGTCCATCTTTTTGCCTGAGAAGCTGGAAGGGTTGAAatatat                                          <  1:865921/91‑1 (MQ=255)                                            cGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCCCATTGGTCCATCTTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCt                                       <  1:585684/91‑1 (MQ=255)                                             gTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCCCATTGGTCCATCTTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTa                                      >  2:335496/1‑91 (MQ=255)                                               cATTATCTGATCAATATAGATATCAATCCTTGGCTGCCCATTGGTCCATCTTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATc                                    >  2:351280/1‑91 (MQ=255)                                                 ttATCTGATCAATATAGATATCAATCCTTGGCTGCCCATTGGTCCATCTTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGt                                  >  2:546395/1‑91 (MQ=255)                                                      tGATCAATATAGATATCAATCCTTGGCTGCCCATTGGTCCATCTTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTc                             >  2:243240/1‑91 (MQ=255)                                                      tGATCAATATAGATATCAATCCTTGGCTGCCCATTGGTCCATCTTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTc                             <  2:659160/91‑1 (MQ=255)                                                       gATCAATATAGATATCAATCCTTGGCTGCCCATTGGTCCATCTTTTTGCCTGAGAAGCTGGaa                                                        >  1:466583/1‑63 (MQ=255)                                                       gATCAATATAGATATCAATCCTTGGCTGCCCATTGGTCCATCTTTTTGCCTGAGAAGCTGGaa                                                        <  2:466583/63‑1 (MQ=255)                                                       gATCAATATAGATATCAATCCTTGGCTGCCCATTGGTCCATCTTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTcc                            >  2:248256/1‑91 (MQ=255)                                                       gATCAATATAGATATCAATCCTTGGCTGCCCAGTGGTCCATCTTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTcc                            >  1:861783/1‑91 (MQ=255)                                                         tCAATATAGATATCAATCCTTGGCTGCCCATTGGTCCATCTTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCgg                          >  1:601628/1‑91 (MQ=255)                                                            atatAGATATCAATCCTTGGCTGCCCATTGGTCCATCTTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCAt                       <  1:655089/91‑1 (MQ=255)                                                            atatAGATATCAATCCTTGGCTGCCCATTGGTCCATCTTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCAt                       <  1:358195/91‑1 (MQ=255)                                                                 gATATCAATCCTTGGCTGCCCATTGGTCCATCTTTTTGCCTGAGAAGCTGGAAGGGTTGAAatata                                           <  2:84622/66‑1 (MQ=255)                                                                 gATATCAATCCTTGGCTGCCCATTGGTCCATCTTTTTGCCTGAGAAGCTGGAAGGGTTGAAatata                                           >  1:84622/1‑66 (MQ=255)                                                                 gATATCAATCCTTGGCTGCCCATTGGTCCATCTTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAACCa                  >  2:816320/1‑91 (MQ=255)                                                                 gATATCAATCCTTGGCTGCCCATTGGTCCATCTTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAACCa                  >  2:297679/1‑91 (MQ=255)                                                                 gATATCAATCCTTGGCTGCCCATTGGTCCATCTTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAACCa                  >  1:228812/1‑91 (MQ=255)                                                                 gATATCAATCCTTGGCTGCCCATTGGTCCATCTTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAACCa                  >  1:646465/1‑91 (MQ=255)                                                                 gATATCAATCCTTGGCTGCCCATTGGTCCATCTTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAACCa                  >  2:620486/1‑91 (MQ=255)                                                                   tatCAATCCTTGGCTGCCCATTGGTCCATCTTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAACCAgg                >  1:802863/1‑91 (MQ=255)                                                                   tatCAATCCTTGGCTGCCCATTGGTCCATCTTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAACCAgg                >  1:769806/1‑91 (MQ=255)                                                                   tatCAATCCTTGGCTGCCCATTGGTCCATCTTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAACCAgg                >  1:697994/1‑91 (MQ=255)                                                                    atCAATCCTTGGCTGCCCATTGGTCCATCTTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAACCAGGa               >  1:128073/1‑91 (MQ=255)                                                                    atCAATCCTTGGCTGCCCATTGGTCCATCTTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAACCAGGa               >  2:678017/1‑91 (MQ=255)                                                                    atCAATCCTTGGCTGCCCATTGGTCCATCTTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAACCAGGa               >  2:459029/1‑91 (MQ=255)                                                                      cAATCCTTGGCTGCCCATTGGTCCATCTTTTTGCCTGAGAAGCTGGa                                                         <  1:433771/47‑1 (MQ=255)                                                                      cAATCCTTGGCTGCCCATTGGTCCATCTTTTTGCCTGAGAAGCTGGa                                                         >  2:433771/1‑47 (MQ=255)                                                                      cAATCCTTGGCTGCCCATTGGTCCATCTTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAACCAGGAAc             >  2:617164/1‑91 (MQ=255)                                                                      cAATCCTTGGCTGCCCATTGGTCCATCTTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAACCAGGAAc             >  1:73629/1‑91 (MQ=255)                                                                        aTCCTTGGCTGCCCATTGGTCCATCTTTTTGCCTGAGAAGCTGGaa                                                        >  1:501680/1‑46 (MQ=255)                                                                        aTCCTTGGCTGCCCATTGGTCCATCTTTTTGCCTGAGAAGCTGGaa                                                        <  2:501680/46‑1 (MQ=255)                                                                        aTCCTTGGCTGCCCATTGGTCCATCTTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAACCAGGAACAt           >  2:653195/1‑91 (MQ=255)                                                                          ccTTGGCTGCCCATTGGTCCATCTTTTTGCCTGAGAAGCTGGAAGGGTTGaaa                                               <  1:727941/53‑1 (MQ=255)                                                                          ccTTGGCTGCCCATTGGTCCATCTTTTTGCCTGAGAAGCTGGAAGGGTTGaaa                                               >  2:727941/1‑53 (MQ=255)                                                                          ccTTGGCTGCCCATTGGTCCATCTTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAACCAGGAACATTg         <  2:746779/91‑1 (MQ=255)                                                                           cTTCGCTGCCCATTGGTCCATCTTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAACCAGGAACATTGc        <  1:839558/91‑1 (MQ=255)                                                                             tGGCTGCCCATTGGTCCATCTTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAACCAGGAACATTGCaa      >  2:382545/1‑91 (MQ=255)                                                                             tGGCTGCCCATTGGTCCATCTTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAACCAGGAACATTGCaa      >  2:805179/1‑91 (MQ=255)                                                                                 tgccCATTGGTCCATCTTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAACCAGGAACATTGCAACCAg  <  1:266720/91‑1 (MQ=255)                                                                                    |                                                                                        ATGCGGCAAGGGCGAATCCGGAAGAATTTGCTACCCCTGTTACCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCTCATTGGTCCATCTTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAACCAGGAACATTGCAACCAG  >  NZ_CP009273/4545513‑4545683

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑