Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	487,218	A→C	K117N (AAA→AAC)	apt →	adenine phosphoribosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	487,218	0	A	C	100.0%	86.0 / NA	24	K117N (AAA→AAC)	apt	adenine phosphoribosyltransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (7/17);  total (7/17)

CGGGCAAACTGCCGCGTGAAACCATCAGTGAAACTTACGACCTGGAATACGGCACCGATCAGCTGGAGATCCACGTTGATGCCATCAAACCGGGCGACAAAGTTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTG  >  NZ_CP009273/487130‑487302                                                                                         |                                                                                     cGGGCAAACTGCCGCGTGAAACCATCAGTGAAACTTACGACCTGGAATACGGCACCGATCAGCTGGAGATCCACGTTGATGCCATCAAccc                                                                                    >  1:129307/1‑91 (MQ=255) cGGGCAAACTGCCGCGTGAAACCATCAGTGAAACTTACGACCTGGAATACGGCACCGATCAGCTGGAGATCCACGTTGATGCCATCAAccc                                                                                    >  2:60538/1‑91 (MQ=255) cGGGCAAACTGCCGCGTGAAACCATCAGTGAAACTTACGACCTGGAATACGGCACCGATCAGCTGGAGATCCACGTTGATGCCATCAAccc                                                                                    >  2:353723/1‑91 (MQ=255) cGGGCAAACTGCCGCGTGAAACCATCAGTGAAACTTACGACCTGGAATACGGCACCGATCAGCTGGAGATCCACGTTGATGCCATCAAccc                                                                                    >  1:772205/1‑91 (MQ=255)  gggCAAACTGCCGCGTGAAACCATCAGTGAAACTTACGACCTGGAATACGGCACCGATCAGCTGGAGATCCACGTTGATGCCATCAACCCg                                                                                   >  2:450657/1‑91 (MQ=255)   ggCAAACTGCCGCGTGAAACCATCAGTGAAACTTACGACCTGGAATACGGCACCGATCAGCTGGAGATCCACGTTGATGCCATCAACCCgg                                                                                  >  2:547115/1‑91 (MQ=255)          tGCCGCGTGAAACCATCAGTGAAACTTACGACCTGGAATACGGCACCGATCAGCTGGAGATCCACGTTGATGCCATCAACCCGGGCGACaa                                                                           <  1:31603/91‑1 (MQ=255)           gCCGCGTGAAACCATCAGTGAAACTTACGACCTGGAATACGGCACCGATCAGCTGGAGATCCACGTTGATGCCATCAACCCGGGCGACaaa                                                                          <  1:123866/91‑1 (MQ=255)                   aaaCCATCAGTGAAACTTACGACCTGGAATACGGCACCGATCAGCTGGAGATCCACGTTGATGCCATCAACCCGGGCGACAAAGTTCtggt                                                                  <  1:794861/91‑1 (MQ=255)                            gTGAAACTTACGACCTGGAATACGGCACCGATCAGCTGGAGATCCACGTTGATGCCATCAACCCGGGCGACAAAGTTCTGGTGGTGgacga                                                         <  1:547115/91‑1 (MQ=255)                              gAAACTTACGACCTGGAATACGGCACCGATCAGCTGGAGATCCACGTTGATGCCATCAACCCGGGCGACAAAGTTCTGGTGGTGGACGAcc                                                       <  1:298111/91‑1 (MQ=255)                                          cTGGAATACGGCACCGATCAGCTGGAGATCCACGTTGATGCCATCAACCCGGGCGACAAAGTTCTGGTGGTGGACGACCTGCTGGCAACcg                                           <  1:456611/91‑1 (MQ=255)                                           tGGAATACGGCACCGATCAGCTGGAGATCCACGTTGATGCCATCAACCCGGGCGACAAAGTTCt                                                                     >  1:801389/1‑64 (MQ=255)                                           tGGAATACGGCACCGATCAGCTGGAGATCCACGTTGATGCCATCAACCCGGGCGACAAAGTTCt                                                                     <  2:801389/64‑1 (MQ=255)                                           tGGAATACGGCACCGATCAGCTGGAGATCCACGTTGATGCCATCAACCCGGGCGACAAAGTTCTGGTGGTGGACGACCTGCTGGCAACcgg                                          >  1:503267/1‑91 (MQ=255)                                               aTACGGCACCGATCAGCTGGAGATCCACGTTGATGCCATCAACCCGGGCGACAAAGTTCTGGTGGTGGACGACCTGCTGGCAACCggcggc                                      <  2:260098/91‑1 (MQ=255)                                               aTACGGCACCGATCAGCTGGAGATCCACGTTGATGCCATCAACCCGGGCGACAAAGTTCTGGTGGTGGACGACCTGCTGGCAACCggcggc                                      <  2:647219/91‑1 (MQ=255)                                               aTACGGCACCGATCAGCTGGAGATCCACGTTGATGCCATCAACCCGGGCGACAAAGTTCTGGTGGCGGACGACCTGCTAGCAACCggcggc                                      <  1:721539/91‑1 (MQ=255)                                                  cGGCACCGATCAGCTGGAGATCCACGTTGATGCCATCAACCCGGGCGACAAAGTTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACt                                   >  1:55956/1‑91 (MQ=255)                                                   ggCACCGATCAGCTGGAGATCCACGTTGATGCCATCAACCCGGGCGACAAAGTTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTa                                  <  1:131254/91‑1 (MQ=255)                                                    gCACCGATCAGCTGGAGATCCACGTTGATGCCATCAACCCGGGCGACAAAGTTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTAt                                 >  2:161139/1‑91 (MQ=255)                                                           tCAGCTGGAGATCCACGTTGATGCCATCAACCCGGGCGACAAAGTTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCg                          <  2:270994/91‑1 (MQ=255)                                                                   agaTCCACGTTGATGCCATCAACCCGGGCGACAAAGTTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTaa                  >  1:753892/1‑91 (MQ=255)                                                                       ccACGTTGATGCCATCAACCCGGGCGACAAAGTTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTg              <  2:623067/91‑1 (MQ=255)                                                                           gTTGATGCCATCAACCCGGGCGACAAAGTTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATcc          <  2:129307/91‑1 (MQ=255)                                                                            gtGATGCCATCAACCCGGGCGACAAAGTTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCcg         <  1:486892/90‑1 (MQ=255)                                                                                 gCCATCAACCCGGGCGACAAAGTTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCgtcgtc    <  1:378163/91‑1 (MQ=255)                                                                                   cATCAACCCGGGCGACAAAGTTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTg  <  1:569108/91‑1 (MQ=255)                                                                                         |                                                                                     CGGGCAAACTGCCGCGTGAAACCATCAGTGAAACTTACGACCTGGAATACGGCACCGATCAGCTGGAGATCCACGTTGATGCCATCAAACCGGGCGACAAAGTTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTG  >  NZ_CP009273/487130‑487302

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑