Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	49,903	T→G	D27E (GAT→GAG)	folA →	type 3 dihydrofolate reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	49,903	0	T	G	100.0%	113.9 / NA	32	D27E (GAT→GAG)	folA	type 3 dihydrofolate reductase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (11/21);  total (11/21)

CAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGG  >  NZ_CP009273/49828‑49989                                                                            |                                                                                       cAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAGCTCGCCTGGTTTaaa                                                                         >  2:96275/1‑91 (MQ=255) cAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAGCTCGCCTGGTTTaaa                                                                         >  2:584993/1‑91 (MQ=255) cAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAGCTCGCCTGGTTTaaa                                                                         >  2:451287/1‑91 (MQ=255) cAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAGCTCGCCTGGTTTaaa                                                                         >  1:499847/1‑91 (MQ=255)      tGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAGCTCGCCTGGTTTAAACGCaa                                                                    <  1:49261/91‑1 (MQ=255)       gATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAGCTCGCCTGGTTTAAACGCAac                                                                   <  2:605251/91‑1 (MQ=255)        aTTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAGCTCGCCTGGTTTAAACGCAaca                                                                  >  2:756805/1‑91 (MQ=255)           gcggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAGCTCGCCTGGTTTAAACGCAACACCt                                                               <  2:645422/91‑1 (MQ=255)           gcggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAGCTCGCCTGGTTTAAACGCAACACCt                                                               <  2:614342/91‑1 (MQ=255)           gcggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAGCTCGCCTGGTTTAAACGCAACACCt                                                               <  1:12849/91‑1 (MQ=255)             ggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAGCTCGCCTGGTTTAAACGCAACACCTta                                                             >  1:591909/1‑91 (MQ=255)             ggcgTTAGCGGTAAATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAGCTCGCCTGGTTTAAACGCAACACCTta                                                             >  2:25504/1‑91 (MQ=255)                  tAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAGCTCGCCTGGTTTAAACGCAACACCTtaaataa                                                        <  2:264342/91‑1 (MQ=255)                          gATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAGCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGa                                                >  2:159420/1‑91 (MQ=255)                          gATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAGCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGa                                                >  1:286296/1‑91 (MQ=255)                              gcgTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAGCTCGCCTGGTTTa                                                                           <  2:140171/60‑1 (MQ=255)                              gcgTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAGCTCGCCTGGTTTa                                                                           >  1:140171/1‑60 (MQ=255)                                gTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAGCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATgg                                          <  1:471749/91‑1 (MQ=255)                                 ttATCGGCATGGAAACCGCCATGCGGTGGAACCTGCCTGCCGAGCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATggg                                         <  2:179652/91‑1 (MQ=255)                                 ttATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAGCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATggg                                         <  2:542509/91‑1 (MQ=255)                                 ttATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAGCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATggg                                         <  1:177824/91‑1 (MQ=255)                                     cGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAGCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGgccgc                                     <  1:243081/91‑1 (MQ=255)                                        cATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAGCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCAt                                  <  2:363542/91‑1 (MQ=255)                                        cATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAGCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCAt                                  <  1:648301/91‑1 (MQ=255)                                            gAAAACGCCATGCCGTGGAACCTGCCTGCCGAGCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCt                              <  2:3604/91‑1 (MQ=255)                                                aCGCCATGCCGTGGAACCTGCCTGCCGAGCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGa                          <  1:451287/91‑1 (MQ=255)                                                       gCCGTGGAACCTGCCTGCCGAGCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGaatcaatc                   <  1:578451/91‑1 (MQ=255)                                                          gTGGAACCTGCCTGCCGAGCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGgt                <  1:657501/91‑1 (MQ=255)                                                             gAACCTGCCTGCCGAGCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGgtc               <  1:511777/89‑1 (MQ=255)                                                             gAACCTGCCTGCCGAGCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGgtc               >  2:511777/1‑89 (MQ=255)                                                                     ctgccGAGCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGcc     <  2:286296/91‑1 (MQ=255)                                                                        ccGAGCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAgg  <  1:404568/91‑1 (MQ=255)                                                                            |                                                                                       CAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGG  >  NZ_CP009273/49828‑49989

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑