Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	634,477	A→G	E26G (GAA→GGA)	ahpC →	alkyl hydroperoxide reductase subunit C

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	634,477	0	A	G	100.0%	97.6 / NA	27	E26G (GAA→GGA)	ahpC	alkyl hydroperoxide reductase subunit C
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (15/12);  total (15/12)

AGATGTCCTTGATTAACACCAAAATTAAACCTTTTAAAAACCAGGCATTCAAAAACGGCGAATTCATCGAAATCACCGAAAAAGATACCGAAGGCCGCTGGAGCGTCTTCTTCTTCTACCCGGCTGACTTTACTTTCGTATGCCCGACCGAACTGGGTGAC  >  NZ_CP009273/634399‑634559                                                                               |                                                                                   aGATGTCCTTGATTAACACCAAAATTAAACCTTTTAAAAACCAGGCATTCAAAAACGGCGAATTCATCGAAATCACCGGAAAAGATACCGa                                                                        >  1:397460/1‑91 (MQ=255) aGATGTCCTTGATTAACACCAAAATTAAACCTTTTAAAAACCAGGCATTCAAAAACGGCGAATTCATCGAAATCACCGGAAAAGATACCGa                                                                        >  1:438539/1‑91 (MQ=255) aGATGTCCTTGATTAACACCAAAATTAAACCTTTTAAAAACCAGGCATTCAAAAACGGCGAATTCATCGAAATCACCGGAAAAGATACCGa                                                                        <  2:397460/91‑1 (MQ=255)   aTGTCCTTGATTAACACCAAAATTAAACCTTTTAAAAACCAGGCATTCAAAAACGGCGAATTCATCGAAATCACCGGAAAAGATACCGAAg                                                                      <  2:438539/91‑1 (MQ=255)   aTGTCCTTGATTAACACCAAAATTAAACCTTTTAAAAACCAGGCATTCAAAAACGGCGAATTCATCGAAATCACCGGAAAAGATACCGAAg                                                                      <  2:214756/91‑1 (MQ=255)    tGTCCTTGATTAACACCAAAATTAAACCTTTTAAAAACCAGGCATTCAAAAACGGCGAATTCATCGAAATCACCGGAAAAGATACCGAAgg                                                                     >  2:457548/1‑91 (MQ=255)     gTCCTTGATTAACACCAAAATTAAACCTTTTAAAAACCAGGCATTCAAAAACGGCGAATTCATCGAAATCACCGGAAAAGATACCGAAGGc                                                                    >  1:455857/1‑91 (MQ=255)      tCCTTGATTAACACCAAAATTAAACCTTTTAAAAACCAGGCATTCAAAAACGGCGAATTCATCGAAATCACCGGAAAAGATACCGAAGGcc                                                                   >  1:277576/1‑91 (MQ=255)       ccTTGATTAACACCAAAATTAAACCTTTTAAAAACCAGGCATTCAAAAACGGCGAATTCATCGAAATCACCGGAAAAGATACCGAAGGCCg                                                                  >  1:156453/1‑91 (MQ=255)       ccTTGATTAACACCAAAATTAAACCTTTTAAAAACCAGGCATTCAAAAACGGCGAATTCATCGAAATCACCGGAAAAGATACCGAAGGCCg                                                                  >  2:697913/1‑91 (MQ=255)       ccTTGATTAACACCAAAATTAAACCTTTTAAAAACCAGGCATTCAAAAACGGCGAATTCATCGAAATCACCGGAAAAGATACCGAAGGCCg                                                                  >  1:350734/1‑91 (MQ=255)       ccTTGATTAACACCAAAATTAAACCTTTTAAAAACCAGGCATTCAAAAACGGCGAATTCATCGAAATCACCGGAAAAGATACCGAAGGCCg                                                                  >  2:155394/1‑91 (MQ=255)         ttGATTAACACCAAAATTAAACCTTTTAAAAACCAGGCATTCAAAAACGGCGAATTCATCGAAATCACCGGAAAAGATACCGAAGGCCGCt                                                                >  1:597505/1‑91 (MQ=255)             ttAACACCAAAATTAAACCTTTTAAAAACCAGGCATTCAAAAACGGCGAATTCATCGAAATCACCGGAAAAGATACCGAAGGCCGCTGGAg                                                            <  2:805426/91‑1 (MQ=255)             ttAACACCAAAATTAAACCTTTTAAAAACCAGGCATTCAAAAACGGCGAATTCATCGAAATCACCGGAAAAGATACCGAAGGCCGCTGGAg                                                            <  2:597505/91‑1 (MQ=255)                     aaaaTTAAACCTTTTAAAAACCAGGCATTCAAAAACGGCGAATTCATCGAAATCACCGGAAAAGATACCGAAGGCCGCTGGAGCGtcttct                                                    >  1:162751/1‑91 (MQ=255)                           aaaCCTTTTAAAAACCAGGCATTCAAAAACGGCGAATTCATCGAAATCACCGGAAAAGATACCGAAGGCCGCTGGAGCGtcttcttcttct                                              >  2:335207/1‑91 (MQ=255)                                 tttAAAAACCAGGCATTCAAAAACGGCGAATTCATCGAAATCACCGGAAAAGATACCGAAGGCCGCTGGAGCGTCTTCTTCTTCTACCCgg                                        >  2:803186/1‑91 (MQ=255)                                 tttAAAAACCAGGCATTCAAAAACGGCGAATTCATCGAAATCACCGGAAAAGATACCGAAGGCCGCTGGAGCGTCTTCTTCTTCTACCCgg                                        >  1:31449/1‑91 (MQ=255)                                    aaaaaCCAGGCATTCAAAAACGGCGAATTCATCGAAATCACCGGAAAAGATACCGAAGGCCGCTGGAGCGTCTTCTTCTTCTACCCGGCTg                                     <  1:573273/91‑1 (MQ=255)                                    aaaaaCCAGGCATTCAAAAACGGCGAATTCATCGAAATCACCGGAAAAGATACCGAAGGCCGCTGGAGCGTCTTCTTCTTCTACCCGGCTg                                     <  2:496151/91‑1 (MQ=255)                                           aGGCATTCAAAAACGGCGAATTCATCGAAATCACCGGAAAAGATACCGAAGGCCGCTGGAGCGTCTTCTTCTTCTACCCGGCTGactttac                              >  2:823271/1‑91 (MQ=255)                                            ggCATTCAAAAACGGCGAATTCATCGAAATCACCGGAAAAGATACCGAAGGCCGCTGGAGCGTCTTCTTCTTCTGCCCGGCTGactttact                             <  2:156453/91‑1 (MQ=255)                                            ggCATTCAAAAACGGCGAATTCATCGAAATCACCGGAAAAGATACCGAAGGCCGCTGGAGCGTCTTCTTCTTCTACCCGGCTGactttact                             <  1:481196/91‑1 (MQ=255)                                                     aaaCGGCGAATTCATCGAAATCACCGGAAAAGATACCGAAGGCCGCTGGAGCGTCTTCTTCTTCTACCCGGCTGACTTTACTTTCGTATGc                    <  1:457548/91‑1 (MQ=255)                                                          gCGAATTCATCGAAATCACCGGAAAAGATACCGAAGGCCGCTGGTGCGTCTTCTTCTTCTACCCGGCTGACTTTACTTTCGTATGCccgac               <  2:455857/91‑1 (MQ=255)                                                                       aaTCACCGGAAAAGATACCGAAGGCCGCTGGAGCGTCTTCTTCTTCTACCCGGCTGACTTTACTTTCGTATGCCCGACCGAACTGGGTGAc  <  1:335207/91‑1 (MQ=255)                                                                               |                                                                                   AGATGTCCTTGATTAACACCAAAATTAAACCTTTTAAAAACCAGGCATTCAAAAACGGCGAATTCATCGAAATCACCGAAAAAGATACCGAAGGCCGCTGGAGCGTCTTCTTCTTCTACCCGGCTGACTTTACTTTCGTATGCCCGACCGAACTGGGTGAC  >  NZ_CP009273/634399‑634559

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑