Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	635,449	T→C	S81P (TCC→CCC)	ahpF →	alkyl hydroperoxide reductase subunit F

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	635,449	0	T	C	100.0%	113.2 / NA	31	S81P (TCC→CCC)	ahpF	alkyl hydroperoxide reductase subunit F
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (22/9);  total (22/9)

AGAAGATAACAGCTTGCCGGTGCGTAAGCCGTCTTTCCTGATCACCAACCCAGGTTCCAACCAGGGGCCACGTTTTGCAGGCTCCCCGCTGGGCCACGAGTTCACCTCGCTGGTACTGGCGTTGCTGTGGACCGGTGGTCATCCGTCGAAAGAAGCGCAGTC  >  NZ_CP009273/635367‑635528                                                                                   |                                                                                agaagaTAACAGCTTGCCGGTGCGTAAGCCGTCTTTCCTGATCACCAACCCAGGTTCCAACCAGGGGCCACGTTTTGCAGGCCCCCCGCTg                                                                         <  1:699064/91‑1 (MQ=255)    agaTAACAGCTTGCCGGTGCGTAAGCCGTCTTTCCTGATCACCAACCCAGGTTCCAACCAGGGGCCACGTTTTGCAGGCCCCCCGCTGGGc                                                                      >  2:53979/1‑91 (MQ=255)    agaTAACAGCTTGCCGGTGCGTAAGCCGTCTTTCCTGATCACCAACCCAGGTTCCAACCAGGGGCCACGTTTTGCAGGCCCCCCGCTGGGc                                                                      >  2:374407/1‑91 (MQ=255)    agaTAACAGCTTGCCGGTGCGTAAGCCGTCTTTCCTGATCACCAACCCAGGTTCCAACCAGGGGCCACGTTTTGCAGGCCCCCCGCTGGGc                                                                      >  2:744471/1‑91 (MQ=255)       tAACAGCTTGCCGGTGCGTAAGCCGTCTTTCCTGATCACCAACCCAGGTTCCAACCAGGGGCCACGTTTTGCAGGCCCCCCGCTGGGCCAc                                                                   >  2:934/1‑91 (MQ=255)                        gTAAGCCGTCTTTCCTGATCACCAACCCAGGTTCCAACCAGGGGCCACGTTTTGCAGGCCCCCCGCTGGGCCACGAGTTCACCTCGCTGGt                                                  >  2:673496/1‑91 (MQ=255)                           aGCCGTCTTTCCTGATCACCAACCCAGGTTCCAACCAGGGGCCACGTTTTGCAGGCCCCCCGCTGGGCCACGAGTTCACCTCGCTGGTACt                                               <  2:26016/91‑1 (MQ=255)                                 cTTTCCTGATCACCAACCCAGGTTCCAACCAGGGGCCACGTTTTGCAGGCCCCCCGCTGGGCCACGAGTTCACCTCGCTGGTACTGGCGtt                                         >  1:201387/1‑91 (MQ=255)                                   ttCCTGATCACCAACCCAGGTTCCAACCAGGGGCCACGTTTTGCAGGCCCCCCGCTGGGCCACGAGTTCACCTCGCTGGTACTGGCGTTGc                                       >  2:194114/1‑91 (MQ=255)                                     ccTGATCACCAACCCAGGTTCCAACCAGGGGCCACGTTTTGCAGGCCCCCCGCTGGGCCACGAGTTCACCTCGCTGGTACTGGCGTTGCtg                                     <  1:53979/91‑1 (MQ=255)                                      cTGATCACCAACCCAGGTTCCAACCAGGGGCCACGTTTTGCAGGCCCCCCGCTGGGCCACGAGTTCACCTCGCTGGTACTGGCGTTGCtgt                                    >  2:354267/1‑91 (MQ=255)                                           cACCAACCCAGGTTCCAACCAGGGGCCACGTTTTGCAGGCCCCCCGCTGGGCCACGAGTTCACCTCGCTGGTACTGGCGTTGCTGTGGAcc                               <  2:522109/91‑1 (MQ=255)                                           cACCAACCCAGGTTCCAACCAGGGGCCACGTTTTGCAGGCCCCCCGCTGGGCCACGAGTTCACCTCGCTGGTACTGGCGTTGCTGTGGAcc                               >  1:537671/1‑91 (MQ=255)                                           cACCAACCCAGGTTCCAACCAGGGGCCACGTTTTGCAGGCCCCCCGCTGGGCCACGAGTTCACCTCGCTGGTACTGGCGTTGCTGTGGAcc                               <  1:404232/91‑1 (MQ=255)                                             ccAACCCAGGTTCCAACCAGGGGCCACGTTTTGCAGGCCCCCCGCTGGGCCACGAGTTCACCTCGCTGGTACTGGCGTTGCTGTGGACCgg                             >  1:708457/1‑91 (MQ=255)                                             ccAACCCAGGTTCCAACCAGGGGCCACGTTTTGCAGGCCCCCCGCTGGGCCACGAGTTCACCTCGCTGGTACTGGCGTTGCTGTGGACCgg                             >  2:849215/1‑91 (MQ=255)                                             ccAACCCAGGTTCCAACCAGGGGCCACGTTTTGCAGGCCCCCCGCTGGGCCACGAGTTCACCTCGCTGGTACTGGCGTTGCTGTGGACCgg                             >  2:585841/1‑91 (MQ=255)                                               aaCCCAGGTTCCAACCAGGGGCCACGTTTTGCAGGCCCCCCGCTGGGCCACGAGTTCACCTCGCTGGTACTGGCGTTGCTGTGGACCggtg                           >  2:125580/1‑91 (MQ=255)                                                    aGGTTCCAACCAGGGGCCACGTTTTGCAGGCCCCCCGCTGGGCCACGAGTTCACCTCGCTg                                                    >  1:634681/1‑61 (MQ=255)                                                    aGGTTCCAACCAGGGGCCACGTTTTGCAGGCCCCCCGCTGGGCCACGAGTTCACCTCGCTg                                                    <  2:634681/61‑1 (MQ=255)                                                          cAACCAGGGGCCACGTTTTGCAGGCCCCCCGCTGGGCCACGAGTTCACCTCGCTGGTACTGGCGTTGCTGTGGACCGGTGGTCATCCGTCg                >  1:184064/1‑91 (MQ=255)                                                          cAACCAGGGGCCACGTTTTGCAGGCCCCCCGCTGGGCCACGAGTTCACCTCGCTGGTACTGGCGTTGCTGTGGACCGGTGGTCATCCGTCg                >  2:502807/1‑91 (MQ=255)                                                           aaCCAGGGGCCACGTTTTGCAGGCCCCCCGCTGGGCCACGAGTTTACCTCGCTGGTACTGGCGTTGCTGTGGACCGGTGGTCATCCGTCGa               >  1:119475/1‑91 (MQ=255)                                                           aaCCAGGGGCCACGTTTTGCAGGCCCCCCGCTGGGCCACGAGTTCACCTCGCTGGTACTGGCGTTGCTGTGGACCGGTGGTCATCCGTCGa               >  2:396414/1‑91 (MQ=255)                                                                ggggCCACGTTTTGCAGGCCCCCCGCTGGGCCACGAGTTCACCTCGCTGGTACTGGCGTTGCTGTGGACCGGTGGTCATCCGTCGAaagaa          >  2:482898/1‑91 (MQ=255)                                                                ggggCCACGTTTTGCAGGCCCCCCGCTGGGCCACGAGTTCACCTCGCTGGTACTGGCGTTGCTGTGGACCGGTGGTCATCCGTCGAaagaa          >  2:741524/1‑91 (MQ=255)                                                                    ccACGTTTTGCAGGCCCCCCGCTGGGCCACGAGTTCACCTCGCTGGTACTGGCGTTGCTGTGGACCGGTGGTCATCCGTCGAAAGAAgcgc      >  2:190314/1‑91 (MQ=255)                                                                    ccACGTTTTGCAGGCCCCCCGCTGGGCCACGAGTTCACCTCGCTGGTACTGGCGTTGCTGTGGACCGGTGGTCATCCGTCGAAAGAAgcgc      <  2:148693/91‑1 (MQ=255)                                                                    ccACGTTTTGCAGGCCCCCCGCTGGGCCACGAGTGCACCTCGCTGGTACTGGCGGTGCTGTGGACCGGTGGTCATCCGTCGAAAGAAgcgc      <  2:347944/91‑1 (MQ=255)                                                                      aCGTTTTGCAGGCCCCCCGCTGGGCCACGAGTTCACCTCGCTGGTACTGGCGTTGCTGTGGACCGGTGGTCATCCGTCGAAAGAAGCGCAg    >  1:620216/1‑91 (MQ=255)                                                                        gTTTTGCAGGCCCCCCGCTGGGCCACGAGTTCACCTCGCTGGTACTGGCGTTGCTGTGGACCGGTGGTCATCCGTCGAAAGAAGCGCAGtc  <  2:445753/91‑1 (MQ=255)                                                                                   |                                                                                AGAAGATAACAGCTTGCCGGTGCGTAAGCCGTCTTTCCTGATCACCAACCCAGGTTCCAACCAGGGGCCACGTTTTGCAGGCTCCCCGCTGGGCCACGAGTTCACCTCGCTGGTACTGGCGTTGCTGTGGACCGGTGGTCATCCGTCGAAAGAAGCGCAGTC  >  NZ_CP009273/635367‑635528

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑