Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	668,149	A→G	N697N (AAT→AAC)	leuS ←	leucine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	668,149	0	A	G	100.0%	114.4 / NA	31	N697N (AAT→AAC)	leuS	leucine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (17/14);  total (17/14)

GGTGTTGAAGGTCTGACGACGGCCGATATCATCGGTCACTTTAGCGATCGTTTTATGCACATCGCGACGCAGCGCTTTCTGATTTTCAGTCAGCGCATCAACGTTCAGTGCCGCAACATCACCTTTTGCTGTGTGCTCGTAAACCAGTTTCCAGACACGTTTCAGGAAG  >  NZ_CP009273/668068‑668236                                                                                  |                                                                                        ggTGTTGAAGGTCTGACGACGGCCGATATCATCGGTCACTTTAGCGATCGTTTTATGCACATCGCGACGCAGCGCTTTCTGGTTTtcagtc                                                                                <  2:826868/91‑1 (MQ=255)   tgtTGAAGGTCTGACGACGGCCGATATCATCGGTCACTTTAGCGATCGTTTTATGCACATCGCGACGCAGCGCTTTCTGGTTTtcagtcag                                                                              <  2:117327/91‑1 (MQ=255)      tGAAGGTCTGACGACGGCCGATATCATCGGTCACTTTAGCGATCGTTTTATGCACATCGCGACGCAGCGCTTTCTGGTTTTCAGTCAgcgc                                                                           >  1:517507/1‑91 (MQ=255)            tCTGACGACGGCCGATATCATCGGTCACTTTAGCGATCGTTTTATGCACATCGCGACGCAGCGCTTTCTGGTTTTCAGTCAGCGCATCAAc                                                                     >  1:182243/1‑91 (MQ=255)            tCTGACGACGGCCGATATCATCGGTCACTTTAGCGATCGTTTTATGCACATCGCGACGCAGCGCTTTCTGGTTTTCAGTCAGCGCATCAAc                                                                     >  1:580183/1‑91 (MQ=255)              tGACGACGGCCGATATCATCGGTCACTTTAGCGATCGTTTTATGCACATCGCGACGCAGCGCTTTCTGGTTTTCAGTCAGCGCATCAACGt                                                                   >  2:322023/1‑91 (MQ=255)              tGACGACGGCCGATATCATCGGTCACTTTAGCGATCGTTTTATGCACATCGCGACGCAGCGCTTTCTGGTTTTCAGTCAGCGCATCAACGt                                                                   >  2:151620/1‑91 (MQ=255)                acgacgGCCGATATCATCGGTCACTTTAGCGATCGTTTTATGCACATCGCGACGCAGCGCTTTCTGGTTTTCAGTCAGCGCATCAACGTTc                                                                 <  1:579424/91‑1 (MQ=255)                    cgGCCGATATCATCGGTCACTTTAGCGATCGTTTTATGCACATCGCGACGCAGCGCTTTCTGGTTTTCAGTCAGCGCATCAACGTTCAGTg                                                             >  1:612027/1‑91 (MQ=255)                      gCCGATATCATCGGTCACTTTAGCGATCGTTTTATGCACATCGCGACGCAGCGCTTTCTGGTTTTCAGTCAGCGCATCAACGTTCAGTGcc                                                           <  1:275604/91‑1 (MQ=255)                      gCCGATATCATCGGTCACTTTAGCGATCGTTTTATGCACATCGCGACGCAGCGCTTTCTGGTTTTCAGTCAGCGCATCAACGTTCAGTGcc                                                           <  2:182243/91‑1 (MQ=255)                             tcatcGGTCACTTTAGCGATCGTTTTATGCACATCGCGACGCAGCGCTTTCTGGTTTTCAGTCAGCGCATCAACGTTCAGTGCCGCAACAt                                                    <  1:229475/91‑1 (MQ=255)                             tcatcGGTCACTTTAGCGATCGTTTTATGCACATCGCGACGCAGCGCTTTCTGGTTTTCAGTCAGCGCATCAACGTTCAGTGCCGCAACAt                                                    <  1:51296/91‑1 (MQ=255)                                    tCACTTTAGCGATCGTTTTATGCACATCGCGACGCAGCGCTTTCTGGTTTTCAGTCAGCGCATCAACGTTCAGTGCCGCAACATCACCttt                                             >  2:12531/1‑91 (MQ=255)                                                tCGTTTTATGCACATCGCGACGCAGCGCTTTCTGGTTTTCAGTCAGCGCATCAACGTTCAGTGCCGCAACATCACCTTTTGCTGTGTGCTc                                 >  2:189009/1‑91 (MQ=255)                                                 cGTTTTATGCACATCGCGACGCAGCGCTTTCTGGTTTtcagtc                                                                                <  2:844477/43‑1 (MQ=255)                                                 cGTTTTATGCACATCGCGACGCAGCGCTTTCTGGTTTtcagtc                                                                                >  1:844477/1‑43 (MQ=255)                                                 cGTTTTATGCACATCGCGACGCAGCGCTTTCTGGTTTTTAGTCAGCGCATCAACGTTCAGTGCCGCAACATCACCTTTTGCTGTGTGCTCg                                >  2:457568/1‑91 (MQ=255)                                                         gCACATCGCGGCGCAGCGCTTTCTGGTTTTCAGTCAGCGCATCAACGTTCAGTGCCGCAACATCACCTTTTGCTGTGTGCTCGTAAACCAg                        >  1:531916/1‑91 (MQ=255)                                                         gCACATCGCGACGCAGCGCTTTCTGGTTTTCAGTCAGCGCATCAACGTTCAGTGCCGCAACATCACCTTTTGCTGTGTGCTCGTAAACCAg                        <  1:189009/91‑1 (MQ=255)                                                            caTCGCGACGCAGCGCTTTCTGGTTTTCAGTCAgcgc                                                                           >  1:534112/1‑37 (MQ=38)                                                            caTCGCGACGCAGCGCTTTCTGGTTTTCAGTCAgcgc                                                                           <  2:534112/37‑1 (MQ=38)                                                              tCGCGACGCAGCGCTTTCTGGTTTTCAGTCAGCGCATCAACGTTCAGTGCCGCAACATCACCTTTTGCTGTGTGCTCGTAAACCAGTTTcc                   >  1:715864/1‑91 (MQ=255)                                                                  gACGCAGCGCTTTCTGGTTTTCAGTCAGCGCATCAACGTTCAGTGCCGCAACATCACCTTTTGCTGTGTGCTCGTAAACCAGTTTCCAGac               <  2:185800/91‑1 (MQ=255)                                                                  gACGCAGCGCTTTCTGGTTTTCAGTCAGCGCATCAACGTTCAGTGCCGCAACATCACCTTTTGCTGTGTGCTCGTAAACCAGTTTCCAGac               <  2:197158/91‑1 (MQ=255)                                                                      cAGCGCTTTCTGGTTTTCAGTCAGCGCATCAACGTTCAGTGCCGCAACATCACCTTTTGCTGTGTGCTCGTAAACCAGTTTCCAGACACGt           >  2:321187/1‑91 (MQ=255)                                                                      cAGCGCTTTCTGGTTTTCAGTCAGCGCATCAACGTTCAGTGCCGCAACATCACCTTTTGCTGTGTGCTCGTAAACCAGTTTCCAGACACGt           >  2:66839/1‑91 (MQ=255)                                                                        gcgcTTTCTGGTTTTCAGTCAGCGCATCAACGTTCAGTGCCGCAACATCACCTTTTGCTGTGTGCTCGTAAACCAGTTTCCAGACACGttt         >  1:225823/1‑91 (MQ=255)                                                                         cgcTTTCTGGTTTTCAGTCAGCGCATCAACGTTCAGTGCCGCAACATCACCTTTTGCTGTGTGCTCGTAAACCAGTTTCCAGACACGTTTc        <  1:486190/91‑1 (MQ=255)                                                                             ttCTGGTTTTCAGTCAGCGCATCAACGTTCAGTGCCGCAACATCACCTTTTGCTGTGTGCTCGTAAACCAGTTTCCAGACACGTTTCAGGa    >  2:232013/1‑91 (MQ=255)                                                                               cTGGTTTTCAGTCAGCGCATCAACGTTCAGTGCCGCAACATCACCTTTTGCTGTGTGCTCGTAAACCAGTTTCCAGACACGTTTCAGGAAg  <  1:151255/91‑1 (MQ=255)                                                                                  |                                                                                        GGTGTTGAAGGTCTGACGACGGCCGATATCATCGGTCACTTTAGCGATCGTTTTATGCACATCGCGACGCAGCGCTTTCTGATTTTCAGTCAGCGCATCAACGTTCAGTGCCGCAACATCACCTTTTGCTGTGTGCTCGTAAACCAGTTTCCAGACACGTTTCAGGAAG  >  NZ_CP009273/668068‑668236

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑