Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	676,968	A→G	D434G (GAT→GGT)	djlC →	co‑chaperone DjlC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	676,968	0	A	G	100.0%	111.6 / NA	30	D434G (GAT→GGT)	djlC	co‑chaperone DjlC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (15/15);  total (15/15)

AATACAAGGCTGGAAGGCGGAGATGCTCATCCATTAGAGGCCATTGTCACCCGGATGCTTTTGACGAAAGTGAAACTCGAGCCGGAGGATGAAGATGTCGATGAGCCAACACCTGAAAATCATGAAGAAAAAAATGATGAGGGTGAAAAACCACAGAGCATTACCAGCAT  >  NZ_CP009273/676880‑677049                                                                                         |                                                                                  aaTACAAGGCTGGAAGGCGGAGATGCTCATCCATTAGAGGCCATTGTCACCCGGATGCTTTTGACCAAAGTGAAACTCGAGCCGGAGGGTg                                                                                 >  1:77848/1‑91 (MQ=255)      aaGGCTGGAAGGCGGAGATGCTCATCCATTAGAGGCCATTGTCACCCGGATGCTTTTGACGAAAGTGAAACTCGAGCCGGAGGGTGAAGAt                                                                            <  2:600609/91‑1 (MQ=255)      aaGGCTGGAAGGCGGAGATGCTCATCCATTAGAGGCCATTGTCACCCGGATGCTTTTGACGAAAGTGAAACTCGAGCCGGAGGGTGAAGAt                                                                            >  1:328145/1‑91 (MQ=255)      aaGGCTGGAAGGCGGAGATGCTCATCCATTAGAGGCCATTGTCACCCGGATGCTTTTGACGAAAGTGAAACTCGAGCCGGAGGGTGAAGAt                                                                            >  1:47495/1‑91 (MQ=255)       aGGCTGGAAGGCGGGGATGCTCATCCATTAGAGGCCATTGTCACCCGGATGCTTTTGACGAAAGTGAAACTCGAGCCGGAGGGTGAAGATg                                                                           >  1:745897/1‑91 (MQ=255)       aGGCTGGAAGGCGGAGATGCTCATCCATTAGAGGCCATTGTCACCCGGATGCTTTTGACGAAAGTGAAACTCGAGCCGGAGGGTGAAGATg                                                                           >  1:499646/1‑91 (MQ=255)       aGGCTGGAAGGCGGAGATGCTCATCCATTAGAGGCCATTGTCACCCGGATGCTTTTGACGAAAGTGAAACTCGAGCCGGAGGGTGAAGATg                                                                           >  2:33694/1‑91 (MQ=255)       aGGCTGGAAGGCGGAGATGCTCATCCATTAGAGGCCATTGTCACCCGGATGCTTTTGACGAAAGTGAAACTCGAGCCGGAGGGTGAAGATg                                                                           >  1:412761/1‑91 (MQ=255)       aGGCTGGAAGGCGGAGATGCTCATCCATTAGAGGCCATTGCCACCCGGATGCTTTTGACGAAAGTGAAACTCGAGCCGGAGGGTGAAGATg                                                                           >  2:366239/1‑91 (MQ=255)            ggAAGGCGGAGATGCTCATCCATTAGAGGCCATTGTCACCCGGATGCTTTTGACGAAAGTGAAACTCGAGCCGGAGGGTGAAGATGTCGAt                                                                      >  2:367029/1‑91 (MQ=255)                           tCATCCATTAGAGGCCATTGTCACCCGGATGCTTTTGACGAAAGTGAAACTCGAGCCGGAGGGTGAAGATGTCGATGAGCCAACACCTGaa                                                       <  1:547021/91‑1 (MQ=255)                                 aTTAGAGGCCATTGTCACCCGGATGCTTTTGACGAAAGTGAAACTCGAGCCGGAGGGTGAAGATGTCGATGAGCCAACACCTGAAAATCAt                                                 >  1:493106/1‑91 (MQ=255)                                         ccATTGTCACCCGGATGCTTTTGACGAAAGTGAAACTCGAGCCGGAGGGTGAAGATGTCGATGAGCCAACACCTGAAAATCATGAAGaaaa                                         >  1:820878/1‑91 (MQ=255)                                          cATTGTCACCCGGATGCTTTTGACGAAAGTGAAACTCGAGCCGGAGGGTGAAGATGTCGATGAGCCAACACCTGAAAATCATGAAGaaaaa                                        >  2:785708/1‑91 (MQ=255)                                                 aCCCGGATGCTTTTGACGAAAGTGAAACTCGAGCCGGAGGGTGAAGATGTCGATGAGCCAACACCTGAAAATCATGAAGAAAAAAatgatg                                 <  1:443828/91‑1 (MQ=255)                                                 aCCCGGATGCTTTTGACGAAAGTGAAACTCGAGCCGGAGGGTGAAGATGTCGATGAGCCAACACCTGAAAATCATGAAGAAAAAAatgatg                                 >  1:56483/1‑91 (MQ=255)                                                 aCCCGGATGCTTTTGACGAAAGTGAAACTCGAGCCGGAGGGTGAAGATGTCGATGAGCCAACACCTGAAAATCATGAAGAAAAAAatgatg                                 <  2:493106/91‑1 (MQ=255)                                                      gATGCTTTTGACGAAAGTGAAACTCGAGCCGGAGGGTGAAGATGTCGATGAGCCAACACCTGAAAATCATGAAGAAAAAAATGATGAGGGt                            <  1:203111/91‑1 (MQ=255)                                                      gATGCTTTTGACGAAAGTGAAACTCGAGCCGGAGGGTGAAGATGTCGATGAGCCAACACCTGAAAATCATGAAGAAAAAAATGATGAGGGt                            >  2:628766/1‑91 (MQ=255)                                                                aCGAAAGTGAAACTCGAGCCGGAGGGTGAAGATGTCGATGAGCCAACACCTGAAAATCATGAAGAAAAAAATGATGAGGGTGAAAAACcac                  <  2:685359/91‑1 (MQ=255)                                                                aCGAAAGTGAAACTCGAGCCGGAGGGTGAAGATGTCGATGAGCCAACACCTGAAAATCATGAAGAAAAAAATGATGAGGGTGAAAAACcac                  <  1:423937/91‑1 (MQ=255)                                                                 cGAAAGTGAAACTCGAGCCGGAGGGTGAAGATGTCGATGAGCCAACACCTGAAAATCATGAAGAAAAAAATGATGAGGGTGAAAAACcaca                 <  1:366239/91‑1 (MQ=255)                                                                 cGAAAGTGAAACTCGAGCCGGAGGGTGAAGATGTCGATGAGCCAACACCTGAAAATCATGAAGAAAAAAATGATGAGGGTGAAAAACcaca                 <  2:56483/91‑1 (MQ=255)                                                                    aaGTGAAACTCGAGCCGGAGGGTGAAGATGTCGATGAGCCAACACCTGAAAATCATGAAGAAAAAAATGATGAGGGTGAAAAACCACagag              >  2:82620/1‑91 (MQ=255)                                                                     aGTGAAACTCGAGCCGGAGGGTGAAGATGTCGATGAGCCAACACCTGAAAATCATGAAGAAAAAAATGATGAGGGTGAAAAACCACAGAGc             <  2:499646/91‑1 (MQ=255)                                                                     aGTGAAACTCGAGCCGGAGGGTGAAGATGTCGATGAGCCAACACCTGAAAATCATGAAGAAAAAAATGATGAGGGTGAAAAACCACAGAGc             <  2:479390/91‑1 (MQ=255)                                                                     aGTGAAACTCGAGCCGGAGGGTGAAGATGTCGATGAGCCAACACCTGAAAATCATGAAGAAAAAAATGATGAGGGTGAAAAACCACAGAGc             <  2:248320/91‑1 (MQ=255)                                                                          aaCTCGAGCCGGAGGGTGAAGATGTCGATGAGCCAACACCTGAAAATCATGAAGAAAAAAATGATGAGGGTGAAAAACCACAGAGCATTAc        <  1:255814/91‑1 (MQ=255)                                                                           aCTCGAGCCGGAGGGTGAAGATGTCGATGAGCCAACACCTGAAAATCATGAAGAAAAAAATGATGAGGGTGAAAAACCACAGAGCATTAcc       <  1:628766/91‑1 (MQ=255)                                                                                aGCCGGAGGGTGAAGATGTCGATGAGCCAACACCTGAAAATCATGAAGAAAAAAATGATGAGGGTGAAAAACCACAGAGCATTACCAGCAt  <  2:77848/91‑1 (MQ=255)                                                                                         |                                                                                  AATACAAGGCTGGAAGGCGGAGATGCTCATCCATTAGAGGCCATTGTCACCCGGATGCTTTTGACGAAAGTGAAACTCGAGCCGGAGGATGAAGATGTCGATGAGCCAACACCTGAAAATCATGAAGAAAAAAATGATGAGGGTGAAAAACCACAGAGCATTACCAGCAT  >  NZ_CP009273/676880‑677049

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑